Do plasma proteins distinguish between liposomes of varying charge density?

Handle URI:
http://hdl.handle.net/10754/598005
Title:
Do plasma proteins distinguish between liposomes of varying charge density?
Authors:
Capriotti, Anna Laura; Caracciolo, Giulio; Cavaliere, Chiara; Foglia, Patrizia; Pozzi, Daniela; Samperi, Roberto; Laganà, Aldo
Abstract:
Cationic liposomes (CLs) are one of the most employed nonviral nanovector systems in gene therapy. However, their transfection efficiency is strongly affected by interactions with plasma components, that lead to the formation of a "protein corona" onto CL surface. The interactions between nanoparticles entering the body and biomolecules have an essential role for their biodistribution. Because the knowledge of proteins adsorbed onto vector surface could be useful in the screening of new, more efficient and more biocompatible liposomal formulations, the behavior of three CLs with different membrane charge densities was investigated. The proteins of the three coronas were identified by nano-liquid chromatography-tandem mass spectrometry, and quantified with label-free spectral counting strategy. Fibrinogen displayed higher association with CLs with high membrane charge density, while apolipoproteins and C4b-binding protein with CLs with low membrane charge density. These results are discussed in terms of the different lipid compositions of CLs and may have a deep biological impact for in vivo applications. Surface charge of nanoparticles is emerging as a relevant factor determining the corona composition after interaction with plasma proteins. Remarkably, it is also shown that the charge of the protein corona formed around CLs is strongly related to their membrane charge density. © 2012 Elsevier B.V.
Citation:
Capriotti AL, Caracciolo G, Cavaliere C, Foglia P, Pozzi D, et al. (2012) Do plasma proteins distinguish between liposomes of varying charge density? Journal of Proteomics 75: 1924–1932. Available: http://dx.doi.org/10.1016/j.jprot.2012.01.003.
Publisher:
Elsevier BV
Journal:
Journal of Proteomics
KAUST Grant Number:
KUK-F1-036-32
Issue Date:
Mar-2012
DOI:
10.1016/j.jprot.2012.01.003
PubMed ID:
22266103
Type:
Article
ISSN:
1874-3919
Sponsors:
This publication is based on work supported by Award No. KUK-F1-036-32, made by King Abdullah University of Science and Technology (KAUST).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorCapriotti, Anna Lauraen
dc.contributor.authorCaracciolo, Giulioen
dc.contributor.authorCavaliere, Chiaraen
dc.contributor.authorFoglia, Patriziaen
dc.contributor.authorPozzi, Danielaen
dc.contributor.authorSamperi, Robertoen
dc.contributor.authorLaganà, Aldoen
dc.date.accessioned2016-02-25T13:10:48Zen
dc.date.available2016-02-25T13:10:48Zen
dc.date.issued2012-03en
dc.identifier.citationCapriotti AL, Caracciolo G, Cavaliere C, Foglia P, Pozzi D, et al. (2012) Do plasma proteins distinguish between liposomes of varying charge density? Journal of Proteomics 75: 1924–1932. Available: http://dx.doi.org/10.1016/j.jprot.2012.01.003.en
dc.identifier.issn1874-3919en
dc.identifier.pmid22266103en
dc.identifier.doi10.1016/j.jprot.2012.01.003en
dc.identifier.urihttp://hdl.handle.net/10754/598005en
dc.description.abstractCationic liposomes (CLs) are one of the most employed nonviral nanovector systems in gene therapy. However, their transfection efficiency is strongly affected by interactions with plasma components, that lead to the formation of a "protein corona" onto CL surface. The interactions between nanoparticles entering the body and biomolecules have an essential role for their biodistribution. Because the knowledge of proteins adsorbed onto vector surface could be useful in the screening of new, more efficient and more biocompatible liposomal formulations, the behavior of three CLs with different membrane charge densities was investigated. The proteins of the three coronas were identified by nano-liquid chromatography-tandem mass spectrometry, and quantified with label-free spectral counting strategy. Fibrinogen displayed higher association with CLs with high membrane charge density, while apolipoproteins and C4b-binding protein with CLs with low membrane charge density. These results are discussed in terms of the different lipid compositions of CLs and may have a deep biological impact for in vivo applications. Surface charge of nanoparticles is emerging as a relevant factor determining the corona composition after interaction with plasma proteins. Remarkably, it is also shown that the charge of the protein corona formed around CLs is strongly related to their membrane charge density. © 2012 Elsevier B.V.en
dc.description.sponsorshipThis publication is based on work supported by Award No. KUK-F1-036-32, made by King Abdullah University of Science and Technology (KAUST).en
dc.publisherElsevier BVen
dc.subjectCationic liposomesen
dc.subjectLabel-free quantitative analysisen
dc.subjectNanoLC-MS/MSen
dc.subjectPlasma proteinsen
dc.subjectProteomicsen
dc.titleDo plasma proteins distinguish between liposomes of varying charge density?en
dc.typeArticleen
dc.identifier.journalJournal of Proteomicsen
dc.contributor.institutionUniversita degli Studi di Roma La Sapienza, Rome, Italyen
kaust.grant.numberKUK-F1-036-32en

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