DNA hosted and aligned in aqueous interstitia of a lamellar liquid crystal – a membrane–biomacromolecule interaction model system

Handle URI:
http://hdl.handle.net/10754/598003
Title:
DNA hosted and aligned in aqueous interstitia of a lamellar liquid crystal – a membrane–biomacromolecule interaction model system
Authors:
Carlsson, Nils; Jonsson, Fabian; Wilhelmsson, L. Marcus; Nordén, Bengt; Åkerman, Björn
Abstract:
We report that DNA molecules can be intercalated and macroscopically oriented in the aqueous interstitia of a lyotropic lamellar liquid crystal. Using UV-vis linear dichroism and fluorescence spectroscopy we show that double-stranded oligonucleotides (25 base pairs) in the water-octanoate-decanol system remain base-paired in the B conformation and are confined in two dimensions, with the helix axis preferentially parallel to the lipid bilayer surfaces but free to rotate within this plane. The degree of helix confinement and the corresponding 2-D orientation can be improved by decreasing the thickness of the water interstitia via the fraction of water in the ternary mixture. Not surprisingly, the corresponding single-stranded oligonucleotides are not aligned, with their persistence length being short in comparison to the lamellar interstitium thickness. We propose this as a model system for studying interactions of DNA-ligand complexes near a lipid bilayer membrane which we demonstrate by using dye probes that are either covalently attached to one end of the oligonucleotide or reversibly bound by intercalation between the base pairs. Three cationic dyes, all strongly bound by intercalation to DNA when free in solution, are found to not bind to DNA but to prefer the membrane surface. The covalently attached Cy5 also binds to the bilayer while Cy3 tends to end-stack to the oligonucleotide duplex. The orientation of Cy5 parallel to the membrane indicates that electrostatic surface binding predominates over insertion into the hydrophobic interior of the membrane. Anionic and zwitterionic dyes (FAM and ROX) are found to remain randomly oriented in the water between the lipid bilayer surfaces. © The Royal Society of Chemistry.
Citation:
Carlsson N, Jonsson F, Wilhelmsson LM, Nordén B, Åkerman B (2013) DNA hosted and aligned in aqueous interstitia of a lamellar liquid crystal – a membrane–biomacromolecule interaction model system. Soft Matter 9: 7951. Available: http://dx.doi.org/10.1039/c3sm50982f.
Publisher:
Royal Society of Chemistry (RSC)
Journal:
Soft Matter
KAUST Grant Number:
KUK-11-008-23
Issue Date:
2013
DOI:
10.1039/c3sm50982f
Type:
Article
ISSN:
1744-683X; 1744-6848
Sponsors:
This work was funded by grants from the Swedish Research Council (grant 212 2067 to BÅ and Linnaeus grant SUPRA 349-2007-8680 to BÅ and BN) and King Abdullah University of Science and Technology (grant KUK-11-008-23 to BN).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorCarlsson, Nilsen
dc.contributor.authorJonsson, Fabianen
dc.contributor.authorWilhelmsson, L. Marcusen
dc.contributor.authorNordén, Bengten
dc.contributor.authorÅkerman, Björnen
dc.date.accessioned2016-02-25T13:10:46Zen
dc.date.available2016-02-25T13:10:46Zen
dc.date.issued2013en
dc.identifier.citationCarlsson N, Jonsson F, Wilhelmsson LM, Nordén B, Åkerman B (2013) DNA hosted and aligned in aqueous interstitia of a lamellar liquid crystal – a membrane–biomacromolecule interaction model system. Soft Matter 9: 7951. Available: http://dx.doi.org/10.1039/c3sm50982f.en
dc.identifier.issn1744-683Xen
dc.identifier.issn1744-6848en
dc.identifier.doi10.1039/c3sm50982fen
dc.identifier.urihttp://hdl.handle.net/10754/598003en
dc.description.abstractWe report that DNA molecules can be intercalated and macroscopically oriented in the aqueous interstitia of a lyotropic lamellar liquid crystal. Using UV-vis linear dichroism and fluorescence spectroscopy we show that double-stranded oligonucleotides (25 base pairs) in the water-octanoate-decanol system remain base-paired in the B conformation and are confined in two dimensions, with the helix axis preferentially parallel to the lipid bilayer surfaces but free to rotate within this plane. The degree of helix confinement and the corresponding 2-D orientation can be improved by decreasing the thickness of the water interstitia via the fraction of water in the ternary mixture. Not surprisingly, the corresponding single-stranded oligonucleotides are not aligned, with their persistence length being short in comparison to the lamellar interstitium thickness. We propose this as a model system for studying interactions of DNA-ligand complexes near a lipid bilayer membrane which we demonstrate by using dye probes that are either covalently attached to one end of the oligonucleotide or reversibly bound by intercalation between the base pairs. Three cationic dyes, all strongly bound by intercalation to DNA when free in solution, are found to not bind to DNA but to prefer the membrane surface. The covalently attached Cy5 also binds to the bilayer while Cy3 tends to end-stack to the oligonucleotide duplex. The orientation of Cy5 parallel to the membrane indicates that electrostatic surface binding predominates over insertion into the hydrophobic interior of the membrane. Anionic and zwitterionic dyes (FAM and ROX) are found to remain randomly oriented in the water between the lipid bilayer surfaces. © The Royal Society of Chemistry.en
dc.description.sponsorshipThis work was funded by grants from the Swedish Research Council (grant 212 2067 to BÅ and Linnaeus grant SUPRA 349-2007-8680 to BÅ and BN) and King Abdullah University of Science and Technology (grant KUK-11-008-23 to BN).en
dc.publisherRoyal Society of Chemistry (RSC)en
dc.titleDNA hosted and aligned in aqueous interstitia of a lamellar liquid crystal – a membrane–biomacromolecule interaction model systemen
dc.typeArticleen
dc.identifier.journalSoft Matteren
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen
kaust.grant.numberKUK-11-008-23en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.