Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability

Handle URI:
http://hdl.handle.net/10754/597861
Title:
Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability
Authors:
Nappini, Silvia; Bonini, Massimo; Bombelli, Francesca Baldelli; Pineider, Francesco; Sangregorio, Claudio; Baglioni, Piero; Nordèn, Bengt
Abstract:
The paper describes the effect of a low-frequency alternating magnetic field (LF-AMF) on the permeability and release properties of large (LUVs) and giant (GUVs) unilamellar vesicles loaded with citrate coated cobalt ferrite nanoparticles (NPs). The citrate shell allows a high loading of NPs in lipid vesicles without modifying their magnetic properties. The increase of magnetic LUVs permeability upon exposure to LF-AMF has been evaluated as the fluorescence self-quenching of carboxyfluorescein (CF) entrapped inside the liposome aqueous pool. Liposome leakage has been monitored as a function of field frequency, time exposure and concentration of the citrate coated NPs. Confocal Laser Scanning Microscopy (CLSM) experiments performed on magnetic GUVs labeled with the fluorescent probe DiIC18 and loaded with Alexa 488-C5-maleimide fluorescent dye provided insights on the release mechanism induced by LF-AMF. The results show that LF-AMF strongly affects vesicles permeability, suggesting the formation of pores in the lipid bilayer due to both hyperthermic effects and nanoparticle oscillations in the vesicles pool at the applied frequency. The behaviour of these magnetic vesicles in the presence of LF-AMF makes this system a good candidate for controlled drug delivery. © 2011 The Royal Society of Chemistry.
Citation:
Nappini S, Bonini M, Bombelli FB, Pineider F, Sangregorio C, et al. (2011) Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability. Soft Matter 7: 1025–1037. Available: http://dx.doi.org/10.1039/c0sm00789g.
Publisher:
Royal Society of Chemistry (RSC)
Journal:
Soft Matter
Issue Date:
2011
DOI:
10.1039/c0sm00789g
Type:
Article
ISSN:
1744-683X; 1744-6848
Sponsors:
Financial support from CSGI and MIUR-PRIN 2008 is acknowledged. We thank Debora Berti and Al Kayal Tamer for their helpful advices for GUVs preparation and Professor R. Udisti and F. Rugi for ICP-OES experiments. Katarina Edwards is also gratefully acknowledged for Cryo-TEM images of magnetoliposomes and Dr C. Innocenti for scientific discussion. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr Tore Eriksson is acknowledged for building the alternating magnetic field generator.
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Full metadata record

DC FieldValue Language
dc.contributor.authorNappini, Silviaen
dc.contributor.authorBonini, Massimoen
dc.contributor.authorBombelli, Francesca Baldellien
dc.contributor.authorPineider, Francescoen
dc.contributor.authorSangregorio, Claudioen
dc.contributor.authorBaglioni, Pieroen
dc.contributor.authorNordèn, Bengten
dc.date.accessioned2016-02-25T12:57:58Zen
dc.date.available2016-02-25T12:57:58Zen
dc.date.issued2011en
dc.identifier.citationNappini S, Bonini M, Bombelli FB, Pineider F, Sangregorio C, et al. (2011) Controlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stability. Soft Matter 7: 1025–1037. Available: http://dx.doi.org/10.1039/c0sm00789g.en
dc.identifier.issn1744-683Xen
dc.identifier.issn1744-6848en
dc.identifier.doi10.1039/c0sm00789gen
dc.identifier.urihttp://hdl.handle.net/10754/597861en
dc.description.abstractThe paper describes the effect of a low-frequency alternating magnetic field (LF-AMF) on the permeability and release properties of large (LUVs) and giant (GUVs) unilamellar vesicles loaded with citrate coated cobalt ferrite nanoparticles (NPs). The citrate shell allows a high loading of NPs in lipid vesicles without modifying their magnetic properties. The increase of magnetic LUVs permeability upon exposure to LF-AMF has been evaluated as the fluorescence self-quenching of carboxyfluorescein (CF) entrapped inside the liposome aqueous pool. Liposome leakage has been monitored as a function of field frequency, time exposure and concentration of the citrate coated NPs. Confocal Laser Scanning Microscopy (CLSM) experiments performed on magnetic GUVs labeled with the fluorescent probe DiIC18 and loaded with Alexa 488-C5-maleimide fluorescent dye provided insights on the release mechanism induced by LF-AMF. The results show that LF-AMF strongly affects vesicles permeability, suggesting the formation of pores in the lipid bilayer due to both hyperthermic effects and nanoparticle oscillations in the vesicles pool at the applied frequency. The behaviour of these magnetic vesicles in the presence of LF-AMF makes this system a good candidate for controlled drug delivery. © 2011 The Royal Society of Chemistry.en
dc.description.sponsorshipFinancial support from CSGI and MIUR-PRIN 2008 is acknowledged. We thank Debora Berti and Al Kayal Tamer for their helpful advices for GUVs preparation and Professor R. Udisti and F. Rugi for ICP-OES experiments. Katarina Edwards is also gratefully acknowledged for Cryo-TEM images of magnetoliposomes and Dr C. Innocenti for scientific discussion. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr Tore Eriksson is acknowledged for building the alternating magnetic field generator.en
dc.publisherRoyal Society of Chemistry (RSC)en
dc.titleControlled drug release under a low frequency magnetic field: effect of the citrate coating on magnetoliposomes stabilityen
dc.typeArticleen
dc.identifier.journalSoft Matteren
dc.contributor.institutionCSGI, Florence, Italyen
dc.contributor.institutionINSTM and Department of Chemistry U. Schiff, Florence, Italyen
dc.contributor.institutionConsiglio Nazionale delle Ricerche, Rome, Italyen
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen
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