Comparative safety of the antifouling compound butenolide and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) to the marine medaka (Oryzias melastigma)

Handle URI:
http://hdl.handle.net/10754/597805
Title:
Comparative safety of the antifouling compound butenolide and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) to the marine medaka (Oryzias melastigma)
Authors:
Chen, Lianguo; Ye, Rui; Xu, Ying; Gao, Zhaoming; Au, Doris W.T.; Qian, Pei-Yuan
Abstract:
This study evaluated the potential adverse effects of butenolide, a promising antifouling compound, using the marine medaka (Oryzias melastigma), a model fish for marine ecotoxicology. The active ingredient used in the commercial antifoulant SeaNine 211, 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) was employed as the positive control. Adult marine medaka (4-month-old) were exposed to various concentrations of butenolide or DCOIT for 28 days and then depurated in clean seawater for 14 days (recovery). A suite of sensitive biomarkers, including hepatic oxidative stress, neuronal signal transmission, endocrine disruption, and reproductive function, was used to measure significant biological effects induced by the chemicals. Compared to DCOIT, chronic exposure to butenolide induced a lower extent of oxidative stress in the liver of male and female medaka. Furthermore, butenolide-exposed fish could recover faster from oxidative stress than fish exposed to DCOIT. Regarding neurotransmission, DCOIT significantly inhibited acetylcholinesterase (AChE) activity in the brain of both male and female medaka, whereas this was not significant for butenolide. In addition, plasma estradiol (E2) level was elevated and testosterone (T) level was decreased in male medaka exposed to DCOIT. This greatly imbalanced sex hormones ratio (E2/T) in exposed males, indicating that DCOIT is a potent endocrine disruptive chemical. In contrast, butenolide induced only moderate effects on sex hormone levels in exposed males, which could be gradually recovered during depuration. Moreover, the endocrine disruptive effect induced by butenolide did not affect normal development of offspring. In contrast, DCOIT-exposed fish exhibited a decrease of egg production and impaired reproductive success. Overall, the above findings demonstrated that chronic exposure to butenolide induced transient, reversible biological effect on marine medaka, while DCOIT could impair reproductive success of fish, as evident by clear alterations of the E2/T ratio. The relatively low toxicity of butenolide on marine biota highlights its promising application in the antifouling industry. The present findings also emphasize gender difference in fish susceptibility to chemical treatment (male>female), which is an important consideration for ecological risk assessment. © 2014 Elsevier B.V.
Citation:
Chen L, Ye R, Xu Y, Gao Z, Au DWT, et al. (2014) Comparative safety of the antifouling compound butenolide and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) to the marine medaka (Oryzias melastigma). Aquatic Toxicology 149: 116–125. Available: http://dx.doi.org/10.1016/j.aquatox.2014.01.023.
Publisher:
Elsevier BV
Journal:
Aquatic Toxicology
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
Apr-2014
DOI:
10.1016/j.aquatox.2014.01.023
PubMed ID:
24583292
Type:
Article
ISSN:
0166-445X
Sponsors:
This study was supported by grants from China Mineral Resources Research and Development Association (COMRRDA12SC01) and from the Research Grant Council of HKSAR government (662413), and the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and the State Key Laboratory in Marine Pollution, City University of Hong Kong.
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Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Lianguoen
dc.contributor.authorYe, Ruien
dc.contributor.authorXu, Yingen
dc.contributor.authorGao, Zhaomingen
dc.contributor.authorAu, Doris W.T.en
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2016-02-25T12:57:01Zen
dc.date.available2016-02-25T12:57:01Zen
dc.date.issued2014-04en
dc.identifier.citationChen L, Ye R, Xu Y, Gao Z, Au DWT, et al. (2014) Comparative safety of the antifouling compound butenolide and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) to the marine medaka (Oryzias melastigma). Aquatic Toxicology 149: 116–125. Available: http://dx.doi.org/10.1016/j.aquatox.2014.01.023.en
dc.identifier.issn0166-445Xen
dc.identifier.pmid24583292en
dc.identifier.doi10.1016/j.aquatox.2014.01.023en
dc.identifier.urihttp://hdl.handle.net/10754/597805en
dc.description.abstractThis study evaluated the potential adverse effects of butenolide, a promising antifouling compound, using the marine medaka (Oryzias melastigma), a model fish for marine ecotoxicology. The active ingredient used in the commercial antifoulant SeaNine 211, 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) was employed as the positive control. Adult marine medaka (4-month-old) were exposed to various concentrations of butenolide or DCOIT for 28 days and then depurated in clean seawater for 14 days (recovery). A suite of sensitive biomarkers, including hepatic oxidative stress, neuronal signal transmission, endocrine disruption, and reproductive function, was used to measure significant biological effects induced by the chemicals. Compared to DCOIT, chronic exposure to butenolide induced a lower extent of oxidative stress in the liver of male and female medaka. Furthermore, butenolide-exposed fish could recover faster from oxidative stress than fish exposed to DCOIT. Regarding neurotransmission, DCOIT significantly inhibited acetylcholinesterase (AChE) activity in the brain of both male and female medaka, whereas this was not significant for butenolide. In addition, plasma estradiol (E2) level was elevated and testosterone (T) level was decreased in male medaka exposed to DCOIT. This greatly imbalanced sex hormones ratio (E2/T) in exposed males, indicating that DCOIT is a potent endocrine disruptive chemical. In contrast, butenolide induced only moderate effects on sex hormone levels in exposed males, which could be gradually recovered during depuration. Moreover, the endocrine disruptive effect induced by butenolide did not affect normal development of offspring. In contrast, DCOIT-exposed fish exhibited a decrease of egg production and impaired reproductive success. Overall, the above findings demonstrated that chronic exposure to butenolide induced transient, reversible biological effect on marine medaka, while DCOIT could impair reproductive success of fish, as evident by clear alterations of the E2/T ratio. The relatively low toxicity of butenolide on marine biota highlights its promising application in the antifouling industry. The present findings also emphasize gender difference in fish susceptibility to chemical treatment (male>female), which is an important consideration for ecological risk assessment. © 2014 Elsevier B.V.en
dc.description.sponsorshipThis study was supported by grants from China Mineral Resources Research and Development Association (COMRRDA12SC01) and from the Research Grant Council of HKSAR government (662413), and the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and the State Key Laboratory in Marine Pollution, City University of Hong Kong.en
dc.publisherElsevier BVen
dc.subjectAntifoulingen
dc.subjectButenolideen
dc.subjectDCOITen
dc.subjectEndocrine disruptionen
dc.subjectMarine medakaen
dc.titleComparative safety of the antifouling compound butenolide and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) to the marine medaka (Oryzias melastigma)en
dc.typeArticleen
dc.identifier.journalAquatic Toxicologyen
dc.contributor.institutionHong Kong University of Science and Technology, Hong Kong, Chinaen
dc.contributor.institutionCity University of Hong Kong, Hong Kong, Chinaen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
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