Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation

Handle URI:
http://hdl.handle.net/10754/597751
Title:
Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation
Authors:
Rose, Nicholas D.; Regan, John M.
Abstract:
© 2015 Elsevier B.V. Geobacter sulfurreducens is one of the dominant bacterial species found in biofilms growing on anodes in bioelectrochemical systems. The intracellular concentrations of reduced and oxidized forms of nicotinamide-adenine dinucleotide (NADH and NAD<sup>+</sup>, respectively) and nicotinamide-adenine dinucleotide phosphate (NADPH and NADP<sup>+</sup>, respectively) as well as adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) were measured in G. sulfurreducens using fumarate, Fe(III)-citrate, or anodes poised at different potentials (110, 10, -90, and -190mV (vs. SHE)) as the electron acceptor. The ratios of CNADH/CNAD+ (0.088±0.022) and CNADPH/CNADP+ (0.268±0.098) were similar under all anode potentials tested and with Fe(III)-citrate (reduced extracellularly). Both ratios significantly increased with fumarate as the electron acceptor (0.331±0.094 for NAD and 1.96±0.37 for NADP). The adenylate energy charge (the fraction of phosphorylation in intracellular adenosine phosphates) was maintained near 0.47 under almost all conditions. Anode-growing biofilms demonstrated a significantly higher molar ratio of ATP/ADP relative to suspended cultures grown on fumarate or Fe(III)-citrate. These results provide evidence that the cellular location of reduction and not the redox potential of the electron acceptor controls the intracellular redox potential in G. sulfurreducens and that biofilm growth alters adenylate phosphorylation.
Citation:
Rose ND, Regan JM (2015) Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation. Bioelectrochemistry 106: 213–220. Available: http://dx.doi.org/10.1016/j.bioelechem.2015.03.003.
Publisher:
Elsevier BV
Journal:
Bioelectrochemistry
KAUST Grant Number:
KUS-I1-003-13
Issue Date:
Dec-2015
DOI:
10.1016/j.bioelechem.2015.03.003
PubMed ID:
25857596
Type:
Article
ISSN:
1567-5394
Sponsors:
This research was supported by Award KUS-I1-003-13 from the KingAbdullah University of Science and Technology (KAUST).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorRose, Nicholas D.en
dc.contributor.authorRegan, John M.en
dc.date.accessioned2016-02-25T12:56:04Zen
dc.date.available2016-02-25T12:56:04Zen
dc.date.issued2015-12en
dc.identifier.citationRose ND, Regan JM (2015) Changes in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variation. Bioelectrochemistry 106: 213–220. Available: http://dx.doi.org/10.1016/j.bioelechem.2015.03.003.en
dc.identifier.issn1567-5394en
dc.identifier.pmid25857596en
dc.identifier.doi10.1016/j.bioelechem.2015.03.003en
dc.identifier.urihttp://hdl.handle.net/10754/597751en
dc.description.abstract© 2015 Elsevier B.V. Geobacter sulfurreducens is one of the dominant bacterial species found in biofilms growing on anodes in bioelectrochemical systems. The intracellular concentrations of reduced and oxidized forms of nicotinamide-adenine dinucleotide (NADH and NAD<sup>+</sup>, respectively) and nicotinamide-adenine dinucleotide phosphate (NADPH and NADP<sup>+</sup>, respectively) as well as adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) were measured in G. sulfurreducens using fumarate, Fe(III)-citrate, or anodes poised at different potentials (110, 10, -90, and -190mV (vs. SHE)) as the electron acceptor. The ratios of CNADH/CNAD+ (0.088±0.022) and CNADPH/CNADP+ (0.268±0.098) were similar under all anode potentials tested and with Fe(III)-citrate (reduced extracellularly). Both ratios significantly increased with fumarate as the electron acceptor (0.331±0.094 for NAD and 1.96±0.37 for NADP). The adenylate energy charge (the fraction of phosphorylation in intracellular adenosine phosphates) was maintained near 0.47 under almost all conditions. Anode-growing biofilms demonstrated a significantly higher molar ratio of ATP/ADP relative to suspended cultures grown on fumarate or Fe(III)-citrate. These results provide evidence that the cellular location of reduction and not the redox potential of the electron acceptor controls the intracellular redox potential in G. sulfurreducens and that biofilm growth alters adenylate phosphorylation.en
dc.description.sponsorshipThis research was supported by Award KUS-I1-003-13 from the KingAbdullah University of Science and Technology (KAUST).en
dc.publisherElsevier BVen
dc.subjectAdenosine phosphateen
dc.subjectGeobacter sulfurreducensen
dc.subjectNicotinamide adenine dinucleotideen
dc.subjectRedox potentialen
dc.titleChanges in phosphorylation of adenosine phosphate and redox state of nicotinamide-adenine dinucleotide (phosphate) in Geobacter sulfurreducens in response to electron acceptor and anode potential variationen
dc.typeArticleen
dc.identifier.journalBioelectrochemistryen
dc.contributor.institutionPennsylvania State University, State College, United Statesen
dc.contributor.institutionInc., Boonton, United Statesen
kaust.grant.numberKUS-I1-003-13en

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