Cell pairing ratio controlled micro-environment with valve-less electrolytic isolation

Handle URI:
http://hdl.handle.net/10754/597742
Title:
Cell pairing ratio controlled micro-environment with valve-less electrolytic isolation
Authors:
Chen, Yu-Chih; Lou, Xia; Ingram, Patrick; Yoon, Euisik
Abstract:
We present a ratio controlled cell-to-cell interaction chip using valve-less isolation. We incorporated electrolysis in a microfluidic channel. In each microfluidic chamber, we loaded two types of different cells at various pairing ratios. More than 80% of the microchambers were successfully loaded with a specific target pairing ratio. For the proof of concept, we have demonstrated the cell-to-cell interaction between prostate cancer cells and muscle stem cells can be controlled by cell pairing ratios through growth factor secretion. The experimental data shows that sealing of microenvironment by air generated from electrolysis does not affect cell viability and cell interaction assay results. © 2012 IEEE.
Citation:
Chen Y-C, Lou X, Ingram P, Yoon E (2012) Cell pairing ratio controlled micro-environment with valve-less electrolytic isolation. 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS). Available: http://dx.doi.org/10.1109/MEMSYS.2012.6170305.
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS)
Issue Date:
Jan-2012
DOI:
10.1109/MEMSYS.2012.6170305
Type:
Conference Paper
Sponsors:
This work was supported in part by the Thermo Fisher Scientific Screening Technology Grant under the Center for Chemical Genomics at the Life Sciences Institute at the University of Michigan, and in part by Academic Excellence Alliance Award from KAUST. The cells are provided by Prof. Buckanovich at the Comprehensive Cancer Center at the University of Michigan.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Yu-Chihen
dc.contributor.authorLou, Xiaen
dc.contributor.authorIngram, Patricken
dc.contributor.authorYoon, Euisiken
dc.date.accessioned2016-02-25T12:55:54Zen
dc.date.available2016-02-25T12:55:54Zen
dc.date.issued2012-01en
dc.identifier.citationChen Y-C, Lou X, Ingram P, Yoon E (2012) Cell pairing ratio controlled micro-environment with valve-less electrolytic isolation. 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS). Available: http://dx.doi.org/10.1109/MEMSYS.2012.6170305.en
dc.identifier.doi10.1109/MEMSYS.2012.6170305en
dc.identifier.urihttp://hdl.handle.net/10754/597742en
dc.description.abstractWe present a ratio controlled cell-to-cell interaction chip using valve-less isolation. We incorporated electrolysis in a microfluidic channel. In each microfluidic chamber, we loaded two types of different cells at various pairing ratios. More than 80% of the microchambers were successfully loaded with a specific target pairing ratio. For the proof of concept, we have demonstrated the cell-to-cell interaction between prostate cancer cells and muscle stem cells can be controlled by cell pairing ratios through growth factor secretion. The experimental data shows that sealing of microenvironment by air generated from electrolysis does not affect cell viability and cell interaction assay results. © 2012 IEEE.en
dc.description.sponsorshipThis work was supported in part by the Thermo Fisher Scientific Screening Technology Grant under the Center for Chemical Genomics at the Life Sciences Institute at the University of Michigan, and in part by Academic Excellence Alliance Award from KAUST. The cells are provided by Prof. Buckanovich at the Comprehensive Cancer Center at the University of Michigan.en
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.titleCell pairing ratio controlled micro-environment with valve-less electrolytic isolationen
dc.typeConference Paperen
dc.identifier.journal2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS)en
dc.contributor.institutionUniversity Michigan Ann Arbor, Ann Arbor, United Statesen
kaust.grant.programAcademic Excellence Alliance (AEA)en
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