Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera

Handle URI:
http://hdl.handle.net/10754/597578
Title:
Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera
Authors:
Kiely, Patrick D.; Call, Douglas F.; Yates, Matthew D.; Regan, John M.; Logan, Bruce E.
Abstract:
Microbial fuel cell (MFC) anode communities often reveal just a few genera, but it is not known to what extent less abundant bacteria could be important for improving performance. We examined the microbial community in an MFC fed with formic acid for more than 1 year and determined using 16S rRNA gene cloning and fluorescent in situ hybridization that members of the Paracoccus genus comprised most (~30%) of the anode community. A Paracoccus isolate obtained from this biofilm (Paracoccus denitrificans strain PS-1) produced only 5.6 mW/m 2, whereas the original mixed culture produced up to 10 mW/m 2. Despite the absence of any Shewanella species in the clone library, we isolated a strain of Shewanella putrefaciens (strain PS-2) from the same biofilm capable of producing a higher-power density (17.4 mW/m2) than the mixed culture, although voltage generation was variable. Our results suggest that the numerical abundance of microorganisms in biofilms cannot be assumed a priori to correlate to capacities of these predominant species for high-power production. Detailed screening of bacterial biofilms may therefore be needed to identify important strains capable of high-power generation for specific substrates. © 2010 Springer-Verlag.
Citation:
Kiely PD, Call DF, Yates MD, Regan JM, Logan BE (2010) Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera. Applied Microbiology and Biotechnology 88: 371–380. Available: http://dx.doi.org/10.1007/s00253-010-2757-2.
Publisher:
Springer Nature
Journal:
Applied Microbiology and Biotechnology
KAUST Grant Number:
KUS-I1-003-13
Issue Date:
15-Jul-2010
DOI:
10.1007/s00253-010-2757-2
PubMed ID:
20632002
Type:
Article
ISSN:
0175-7598; 1432-0614
Sponsors:
This research was supported by Award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST) and the National Renewable Energy Laboratory (RFH-7-77623-01).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorKiely, Patrick D.en
dc.contributor.authorCall, Douglas F.en
dc.contributor.authorYates, Matthew D.en
dc.contributor.authorRegan, John M.en
dc.contributor.authorLogan, Bruce E.en
dc.date.accessioned2016-02-25T12:42:25Zen
dc.date.available2016-02-25T12:42:25Zen
dc.date.issued2010-07-15en
dc.identifier.citationKiely PD, Call DF, Yates MD, Regan JM, Logan BE (2010) Anodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant genera. Applied Microbiology and Biotechnology 88: 371–380. Available: http://dx.doi.org/10.1007/s00253-010-2757-2.en
dc.identifier.issn0175-7598en
dc.identifier.issn1432-0614en
dc.identifier.pmid20632002en
dc.identifier.doi10.1007/s00253-010-2757-2en
dc.identifier.urihttp://hdl.handle.net/10754/597578en
dc.description.abstractMicrobial fuel cell (MFC) anode communities often reveal just a few genera, but it is not known to what extent less abundant bacteria could be important for improving performance. We examined the microbial community in an MFC fed with formic acid for more than 1 year and determined using 16S rRNA gene cloning and fluorescent in situ hybridization that members of the Paracoccus genus comprised most (~30%) of the anode community. A Paracoccus isolate obtained from this biofilm (Paracoccus denitrificans strain PS-1) produced only 5.6 mW/m 2, whereas the original mixed culture produced up to 10 mW/m 2. Despite the absence of any Shewanella species in the clone library, we isolated a strain of Shewanella putrefaciens (strain PS-2) from the same biofilm capable of producing a higher-power density (17.4 mW/m2) than the mixed culture, although voltage generation was variable. Our results suggest that the numerical abundance of microorganisms in biofilms cannot be assumed a priori to correlate to capacities of these predominant species for high-power production. Detailed screening of bacterial biofilms may therefore be needed to identify important strains capable of high-power generation for specific substrates. © 2010 Springer-Verlag.en
dc.description.sponsorshipThis research was supported by Award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST) and the National Renewable Energy Laboratory (RFH-7-77623-01).en
dc.publisherSpringer Natureen
dc.subjectCommunity analysisen
dc.subjectFormic aciden
dc.subjectMicrobial fuel cellen
dc.subjectParacoccus denitrificansen
dc.titleAnodic biofilms in microbial fuel cells harbor low numbers of higher-power-producing bacteria than abundant generaen
dc.typeArticleen
dc.identifier.journalApplied Microbiology and Biotechnologyen
dc.contributor.institutionPennsylvania State University, State College, United Statesen
kaust.grant.numberKUS-I1-003-13en

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