Analysis of Pacific oyster larval proteome and its response to high-CO2

Handle URI:
http://hdl.handle.net/10754/597560
Title:
Analysis of Pacific oyster larval proteome and its response to high-CO2
Authors:
Dineshram, R.; Wong, Kelvin K.W.; Xiao, Shu; Yu, Ziniu; Qian, Pei Yuan; Thiyagarajan, Vengatesen
Abstract:
Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO2 due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO2. Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. © 2012 Elsevier Ltd.
Citation:
Dineshram R, Wong KKW, Xiao S, Yu Z, Qian PY, et al. (2012) Analysis of Pacific oyster larval proteome and its response to high-CO2. Marine Pollution Bulletin 64: 2160–2167. Available: http://dx.doi.org/10.1016/j.marpolbul.2012.07.043.
Publisher:
Elsevier BV
Journal:
Marine Pollution Bulletin
Issue Date:
Oct-2012
DOI:
10.1016/j.marpolbul.2012.07.043
PubMed ID:
22921897
Type:
Article
ISSN:
0025-326X
Sponsors:
We thank A. Ishimatsu (Nagasaki University, Japan), J.M. Hall-Spencer (University of Plymouth, UK), Sam Dupont (EPOCA, Sweden), Richard Zeebe (University of Hawaii, USA), Gray Williams and Kenneth Leung (The University of Hong Kong, Hong Kong) for their valuable discussions and for their support in setting up the ocean acidification facilities. We thank Mr. Fu (in oyster hatchery) for his support on oyster larval culture. This study was primarily supported by a Grant from the HKSAR-RGC (No. 778309M) and partially from the Area of Excellency Project grant (No. AoE/P-04/2004) to VT. The oyster larval culture facilities located in Zhanjiang (China) hatchery was partially funded by the 973 program to ZY. The proteomic part of this study was partially supported by KAUST-HKUST project funded to PYQ.
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Full metadata record

DC FieldValue Language
dc.contributor.authorDineshram, R.en
dc.contributor.authorWong, Kelvin K.W.en
dc.contributor.authorXiao, Shuen
dc.contributor.authorYu, Ziniuen
dc.contributor.authorQian, Pei Yuanen
dc.contributor.authorThiyagarajan, Vengatesenen
dc.date.accessioned2016-02-25T12:42:04Zen
dc.date.available2016-02-25T12:42:04Zen
dc.date.issued2012-10en
dc.identifier.citationDineshram R, Wong KKW, Xiao S, Yu Z, Qian PY, et al. (2012) Analysis of Pacific oyster larval proteome and its response to high-CO2. Marine Pollution Bulletin 64: 2160–2167. Available: http://dx.doi.org/10.1016/j.marpolbul.2012.07.043.en
dc.identifier.issn0025-326Xen
dc.identifier.pmid22921897en
dc.identifier.doi10.1016/j.marpolbul.2012.07.043en
dc.identifier.urihttp://hdl.handle.net/10754/597560en
dc.description.abstractMost calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO2 due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO2. Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae. © 2012 Elsevier Ltd.en
dc.description.sponsorshipWe thank A. Ishimatsu (Nagasaki University, Japan), J.M. Hall-Spencer (University of Plymouth, UK), Sam Dupont (EPOCA, Sweden), Richard Zeebe (University of Hawaii, USA), Gray Williams and Kenneth Leung (The University of Hong Kong, Hong Kong) for their valuable discussions and for their support in setting up the ocean acidification facilities. We thank Mr. Fu (in oyster hatchery) for his support on oyster larval culture. This study was primarily supported by a Grant from the HKSAR-RGC (No. 778309M) and partially from the Area of Excellency Project grant (No. AoE/P-04/2004) to VT. The oyster larval culture facilities located in Zhanjiang (China) hatchery was partially funded by the 973 program to ZY. The proteomic part of this study was partially supported by KAUST-HKUST project funded to PYQ.en
dc.publisherElsevier BVen
dc.subjectEnvironmental proteomicsen
dc.subjectHigh-CO2en
dc.subjectLarval proteomeen
dc.subjectOcean acidificationen
dc.subjectPacific oysteren
dc.subjectVeligeren
dc.titleAnalysis of Pacific oyster larval proteome and its response to high-CO2en
dc.typeArticleen
dc.identifier.journalMarine Pollution Bulletinen
dc.contributor.institutionThe University of Hong Kong, Pokfulam, Hong Kongen
dc.contributor.institutionSouth China Seas Institute of Oceanography Chinese Academy of Sciences, Guangzhou, Chinaen
dc.contributor.institutionHong Kong University of Science and Technology, Hong Kong, Chinaen

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