Alamethicin Suppresses Methanogenesis and Promotes Acetogenesis in Bioelectrochemical Systems

Handle URI:
http://hdl.handle.net/10754/597484
Title:
Alamethicin Suppresses Methanogenesis and Promotes Acetogenesis in Bioelectrochemical Systems
Authors:
Zhu, Xiuping; Siegert, Michael; Yates, Matthew D.; Logan, Bruce E.
Abstract:
Microbial electrosynthesis (MES) systems with mixed cultures often generate a variety of gaseous and soluble chemicals. Methane is the primary end product in mixed-culture MES because it is the thermodynamically most favorable reduction product of CO2. Here, we show that the peptaibol alamethicin selectively suppressed the growth of methanogens in mixed-culture MES systems, resulting in a shift of the solution and cathode communities to an acetate-producing system dominated by Sporomusa, a known acetogenic genus in MES systems. Archaea in the methane-producing control were dominated by Methanobrevibacter species, but no Archaea were detected in the alamethicin-treated reactors. No methane was detected in the mixed-culture reactors treated with alamethicin over 10 cycles (∼ 3 days each). Instead, acetate was produced at an average rate of 115 nmol ml(-1) day(-1), similar to the rate reported previously for pure cultures of Sporomusa ovata on biocathodes. Mixed-culture control reactors without alamethicin generated methane at nearly 100% coulombic recovery, and no acetate was detected. These results show that alamethicin is effective for the suppression of methanogen growth in MES systems and that its use enables the production of industrially relevant organic compounds by the inhibition of methanogenesis.
Citation:
Zhu X, Siegert M, Yates MD, Logan BE (2015) Alamethicin Suppresses Methanogenesis and Promotes Acetogenesis in Bioelectrochemical Systems. Appl Environ Microbiol 81: 3863–3868. Available: http://dx.doi.org/10.1128/AEM.00594-15.
Publisher:
American Society for Microbiology
Journal:
Applied and Environmental Microbiology
KAUST Grant Number:
KUS-I1-003-13
Issue Date:
27-Mar-2015
DOI:
10.1128/AEM.00594-15
PubMed ID:
25819972
PubMed Central ID:
PMC4421063
Type:
Article
ISSN:
0099-2240; 1098-5336
Sponsors:
This research was supported by award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST) and the Global Climate and Energy Program (GCEP).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorZhu, Xiupingen
dc.contributor.authorSiegert, Michaelen
dc.contributor.authorYates, Matthew D.en
dc.contributor.authorLogan, Bruce E.en
dc.date.accessioned2016-02-25T12:40:38Zen
dc.date.available2016-02-25T12:40:38Zen
dc.date.issued2015-03-27en
dc.identifier.citationZhu X, Siegert M, Yates MD, Logan BE (2015) Alamethicin Suppresses Methanogenesis and Promotes Acetogenesis in Bioelectrochemical Systems. Appl Environ Microbiol 81: 3863–3868. Available: http://dx.doi.org/10.1128/AEM.00594-15.en
dc.identifier.issn0099-2240en
dc.identifier.issn1098-5336en
dc.identifier.pmid25819972en
dc.identifier.doi10.1128/AEM.00594-15en
dc.identifier.urihttp://hdl.handle.net/10754/597484en
dc.description.abstractMicrobial electrosynthesis (MES) systems with mixed cultures often generate a variety of gaseous and soluble chemicals. Methane is the primary end product in mixed-culture MES because it is the thermodynamically most favorable reduction product of CO2. Here, we show that the peptaibol alamethicin selectively suppressed the growth of methanogens in mixed-culture MES systems, resulting in a shift of the solution and cathode communities to an acetate-producing system dominated by Sporomusa, a known acetogenic genus in MES systems. Archaea in the methane-producing control were dominated by Methanobrevibacter species, but no Archaea were detected in the alamethicin-treated reactors. No methane was detected in the mixed-culture reactors treated with alamethicin over 10 cycles (∼ 3 days each). Instead, acetate was produced at an average rate of 115 nmol ml(-1) day(-1), similar to the rate reported previously for pure cultures of Sporomusa ovata on biocathodes. Mixed-culture control reactors without alamethicin generated methane at nearly 100% coulombic recovery, and no acetate was detected. These results show that alamethicin is effective for the suppression of methanogen growth in MES systems and that its use enables the production of industrially relevant organic compounds by the inhibition of methanogenesis.en
dc.description.sponsorshipThis research was supported by award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST) and the Global Climate and Energy Program (GCEP).en
dc.publisherAmerican Society for Microbiologyen
dc.subject.meshBioelectric Energy Sourcesen
dc.titleAlamethicin Suppresses Methanogenesis and Promotes Acetogenesis in Bioelectrochemical Systemsen
dc.typeArticleen
dc.identifier.journalApplied and Environmental Microbiologyen
dc.identifier.pmcidPMC4421063en
dc.contributor.institutionDepartment of Civil and Environmental Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA.en
dc.contributor.institutionDepartment of Civil and Environmental Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.en
dc.contributor.institutionDepartment of Civil and Environmental Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA blogan@psu.edu.en
kaust.grant.numberKUS-I1-003-13en

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