Activity and specificity of TRV-mediated gene editing in plants

Handle URI:
http://hdl.handle.net/10754/597449
Title:
Activity and specificity of TRV-mediated gene editing in plants
Authors:
Ali, Zahir ( 0000-0002-7814-8908 ) ; Abulfaraj, Aala Abdulaziz Hussien; Piatek, Marek J.; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
© 2015 Taylor and Francis Group, LLC. Plant trait engineering requires efficient targeted genome-editing technologies. Clustered regularly interspaced palindromic repeats (CRISPRs)/ CRISPR associated (Cas) type II system is used for targeted genome-editing applications across eukaryotic species including plants. Delivery of genome engineering reagents and recovery of mutants remain challenging tasks for in planta applications. Recently, we reported the development of Tobacco rattle virus (TRV)-mediated genome editing in Nicotiana benthamiana. TRV infects the growing points and possesses small genome size; which facilitate cloning, multiplexing, and agroinfections. Here, we report on the persistent activity and specificity of the TRV-mediated CRISPR/Cas9 system for targeted modification of the Nicotiana benthamiana genome. Our data reveal the persistence of the TRVmediated Cas9 activity for up to 30 d post-agroinefection. Further, our data indicate that TRV-mediated genome editing exhibited no off-target activities at potential off-targets indicating the precision of the system for plant genome engineering. Taken together, our data establish the feasibility and exciting possibilities of using virus-mediated CRISPR/Cas9 for targeted engineering of plant genomes.
Citation:
Ali Z, Abul-faraj A, Piatek M, Mahfouz MM (2015) Activity and specificity of TRV-mediated gene editing in plants. Plant Signaling & Behavior 10: e1044191. Available: http://dx.doi.org/10.1080/15592324.2015.1044191.
Publisher:
Informa UK Limited
Journal:
Plant Signaling & Behavior
Issue Date:
3-Jun-2015
DOI:
10.1080/15592324.2015.1044191
PubMed ID:
26039254
Type:
Article
ISSN:
1559-2324
Sponsors:
We would like to thank members of the laboratory for genome engineering at KAUST for helpful discussions and comments. This study is supported by King Abdullah University of Science and Technology (KAUST).
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorAli, Zahiren
dc.contributor.authorAbulfaraj, Aala Abdulaziz Hussienen
dc.contributor.authorPiatek, Marek J.en
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2016-02-25T12:33:28Zen
dc.date.available2016-02-25T12:33:28Zen
dc.date.issued2015-06-03en
dc.identifier.citationAli Z, Abul-faraj A, Piatek M, Mahfouz MM (2015) Activity and specificity of TRV-mediated gene editing in plants. Plant Signaling & Behavior 10: e1044191. Available: http://dx.doi.org/10.1080/15592324.2015.1044191.en
dc.identifier.issn1559-2324en
dc.identifier.pmid26039254en
dc.identifier.doi10.1080/15592324.2015.1044191en
dc.identifier.urihttp://hdl.handle.net/10754/597449en
dc.description.abstract© 2015 Taylor and Francis Group, LLC. Plant trait engineering requires efficient targeted genome-editing technologies. Clustered regularly interspaced palindromic repeats (CRISPRs)/ CRISPR associated (Cas) type II system is used for targeted genome-editing applications across eukaryotic species including plants. Delivery of genome engineering reagents and recovery of mutants remain challenging tasks for in planta applications. Recently, we reported the development of Tobacco rattle virus (TRV)-mediated genome editing in Nicotiana benthamiana. TRV infects the growing points and possesses small genome size; which facilitate cloning, multiplexing, and agroinfections. Here, we report on the persistent activity and specificity of the TRV-mediated CRISPR/Cas9 system for targeted modification of the Nicotiana benthamiana genome. Our data reveal the persistence of the TRVmediated Cas9 activity for up to 30 d post-agroinefection. Further, our data indicate that TRV-mediated genome editing exhibited no off-target activities at potential off-targets indicating the precision of the system for plant genome engineering. Taken together, our data establish the feasibility and exciting possibilities of using virus-mediated CRISPR/Cas9 for targeted engineering of plant genomes.en
dc.description.sponsorshipWe would like to thank members of the laboratory for genome engineering at KAUST for helpful discussions and comments. This study is supported by King Abdullah University of Science and Technology (KAUST).en
dc.publisherInforma UK Limiteden
dc.subjectCRISPR/Cas9 systemen
dc.subjectPlant genome engineeringen
dc.subjectSynthetic site-specific nucleases (SSNs)en
dc.subjectTRVen
dc.subjectViral-mediated genome editingen
dc.titleActivity and specificity of TRV-mediated gene editing in plantsen
dc.typeArticleen
dc.identifier.journalPlant Signaling & Behavioren
dc.contributor.institutionKing Abdullah University of Science and Technology, Jeddah, Saudi Arabiaen
kaust.authorAli, Zahiren
kaust.authorAbulfaraj, Aala Abdulaziz Hussienen
kaust.authorPiatek, Marek J.en
kaust.authorMahfouz, Magdy M.en

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