A New Modular Approach to Nanoassembly: Stable and Addressable DNA Nanoconstructs via Orthogonal Click Chemistries

Handle URI:
http://hdl.handle.net/10754/597344
Title:
A New Modular Approach to Nanoassembly: Stable and Addressable DNA Nanoconstructs via Orthogonal Click Chemistries
Authors:
Gerrard, Simon R.; Hardiman, Claire; Shelbourne, Montserrat; Nandhakumar, Iris; Nordén, Bengt; Brown, Tom
Abstract:
Thermodynamic instability is a problem when assembling and purifying complex DNA nanostructures formed by hybridization alone. To address this issue, we have used photochemical fixation and orthogonal copper-free, ring-strain-promoted, click chemistry for the synthesis of dimeric, trimeric, and oligomeric modular DNA scaffolds from cyclic, double-stranded, 80-mer DNA nanoconstructs. This particular combination of orthogonal click reactions was more effective for nanoassembly than others explored. The complex nanostructures are stable to heat and denaturation agents and can therefore be purified and characterized. They are addressable in a sequence-specific manner by triplex formation, and they can be reversibly and selectively deconstructed. Nanostructures utilizing this orthogonal, chemical fixation methodology can be used as building blocks for nanomachines and functional DNA nanoarchitectures. © 2012 American Chemical Society.
Citation:
Gerrard SR, Hardiman C, Shelbourne M, Nandhakumar I, Nordén B, et al. (2012) A New Modular Approach to Nanoassembly: Stable and Addressable DNA Nanoconstructs via Orthogonal Click Chemistries . ACS Nano 6: 9221–9228. Available: http://dx.doi.org/10.1021/nn3035759.
Publisher:
American Chemical Society (ACS)
Journal:
ACS Nano
Issue Date:
23-Oct-2012
DOI:
10.1021/nn3035759
PubMed ID:
22989197
Type:
Article
ISSN:
1936-0851; 1936-086X
Sponsors:
We gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for a grant awarded to B. Norden, and the U.K. BBSRC for the sLoLa grant to T. Brown. "Extending the boundaries of nucleic acid chemistry." Oligonucleotides were synthesised by ATDBio Ltd. We thank Dr. G. John Langley and Julie Herniman for supervision of the oligonucleotide mass spectrometry facility.
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorGerrard, Simon R.en
dc.contributor.authorHardiman, Claireen
dc.contributor.authorShelbourne, Montserraten
dc.contributor.authorNandhakumar, Irisen
dc.contributor.authorNordén, Bengten
dc.contributor.authorBrown, Tomen
dc.date.accessioned2016-02-25T12:31:10Zen
dc.date.available2016-02-25T12:31:10Zen
dc.date.issued2012-10-23en
dc.identifier.citationGerrard SR, Hardiman C, Shelbourne M, Nandhakumar I, Nordén B, et al. (2012) A New Modular Approach to Nanoassembly: Stable and Addressable DNA Nanoconstructs via Orthogonal Click Chemistries . ACS Nano 6: 9221–9228. Available: http://dx.doi.org/10.1021/nn3035759.en
dc.identifier.issn1936-0851en
dc.identifier.issn1936-086Xen
dc.identifier.pmid22989197en
dc.identifier.doi10.1021/nn3035759en
dc.identifier.urihttp://hdl.handle.net/10754/597344en
dc.description.abstractThermodynamic instability is a problem when assembling and purifying complex DNA nanostructures formed by hybridization alone. To address this issue, we have used photochemical fixation and orthogonal copper-free, ring-strain-promoted, click chemistry for the synthesis of dimeric, trimeric, and oligomeric modular DNA scaffolds from cyclic, double-stranded, 80-mer DNA nanoconstructs. This particular combination of orthogonal click reactions was more effective for nanoassembly than others explored. The complex nanostructures are stable to heat and denaturation agents and can therefore be purified and characterized. They are addressable in a sequence-specific manner by triplex formation, and they can be reversibly and selectively deconstructed. Nanostructures utilizing this orthogonal, chemical fixation methodology can be used as building blocks for nanomachines and functional DNA nanoarchitectures. © 2012 American Chemical Society.en
dc.description.sponsorshipWe gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for a grant awarded to B. Norden, and the U.K. BBSRC for the sLoLa grant to T. Brown. "Extending the boundaries of nucleic acid chemistry." Oligonucleotides were synthesised by ATDBio Ltd. We thank Dr. G. John Langley and Julie Herniman for supervision of the oligonucleotide mass spectrometry facility.en
dc.publisherAmerican Chemical Society (ACS)en
dc.subjectcopper-free clicken
dc.subjectDNA nanotechnologyen
dc.subjectorthogonal chemical fixationen
dc.subjectphotochemical cross-linkingen
dc.titleA New Modular Approach to Nanoassembly: Stable and Addressable DNA Nanoconstructs via Orthogonal Click Chemistriesen
dc.typeArticleen
dc.identifier.journalACS Nanoen
dc.contributor.institutionUniversity of Southampton, Southampton, United Kingdomen
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen

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