A new method to reconstruct fish diet and movement patterns from δ 13 C values in otolith amino acids

Handle URI:
http://hdl.handle.net/10754/597343
Title:
A new method to reconstruct fish diet and movement patterns from δ 13 C values in otolith amino acids
Authors:
McMahon, Kelton W.; Fogel, Marilyn L.; Johnson, Beverly J.; Houghton, Leah A.; Thorrold, Simon R.; Gillanders, Bronwyn
Abstract:
Fish ecologists have used geochemical values in otoliths to examine habitat use, migration, and population connectivity for decades. However, it remains difficult to determine an unambiguous dietary δ 13C signature from bulk analysis of otolith. Studies to date have focused on the aragonite component of otoliths with less attention paid to the organic fraction. We describe the application of compound-specific stable isotope analysis (SIA) to analyze amino acid (AA) δ 13C values from small amounts (<1 mg) of otolith powder. We examined δ 13C values of otolith and muscle AAs from a reef-associated snapper (Lutjanus ehrenbergii (Peters, 1869)) collected along a carbon isotope gradient (isoscape) from seagrass beds to coral reefs. Carbon isotope values in otolith and muscle samples were highly correlated within and among coastal habitats. Moreover, δ 13C values of otolith AAs provided a purely dietary record that avoided dilution from dissolved inorganic carbon. Otolith AAs served as a robust tracer of δ 13C values at the base of the food web, making compound-specific SIA a powerful tool for dietary reconstructions and tracking the movement of fishes across isoscapes.
Citation:
McMahon KW, Fogel ML, Johnson BJ, Houghton LA, Thorrold SR, et al. (2011) A new method to reconstruct fish diet and movement patterns from δ 13 C values in otolith amino acids . Canadian Journal of Fisheries and Aquatic Sciences 68: 1330–1340. Available: http://dx.doi.org/10.1139/F2011-070.
Publisher:
Canadian Science Publishing
Journal:
Canadian Journal of Fisheries and Aquatic Sciences
KAUST Grant Number:
USA 00002; KSA 00011
Issue Date:
Aug-2011
DOI:
10.1139/F2011-070
Type:
Article
ISSN:
0706-652X; 1205-7533
Sponsors:
The authors thank M. Berumen for field assistance and the crew of Dream Divers, Jeddah, Saudi Arabia, for boat and dive operation assistance. The site map was created by C. Braun. This research was based on work supported by awards USA 00002 and KSA 00011 made by King Abdullah University of Science and Technology (KAUST). Additional funding was provided by Woods Hole Oceanographic Institution and an International Society for Reef Studies - Ocean Conservancy Coral Reef Fellowship. K. McMahon received support from the National Science Foundation Graduate Research Fellowship Program.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorMcMahon, Kelton W.en
dc.contributor.authorFogel, Marilyn L.en
dc.contributor.authorJohnson, Beverly J.en
dc.contributor.authorHoughton, Leah A.en
dc.contributor.authorThorrold, Simon R.en
dc.contributor.authorGillanders, Bronwynen
dc.date.accessioned2016-02-25T12:31:08Zen
dc.date.available2016-02-25T12:31:08Zen
dc.date.issued2011-08en
dc.identifier.citationMcMahon KW, Fogel ML, Johnson BJ, Houghton LA, Thorrold SR, et al. (2011) A new method to reconstruct fish diet and movement patterns from δ 13 C values in otolith amino acids . Canadian Journal of Fisheries and Aquatic Sciences 68: 1330–1340. Available: http://dx.doi.org/10.1139/F2011-070.en
dc.identifier.issn0706-652Xen
dc.identifier.issn1205-7533en
dc.identifier.doi10.1139/F2011-070en
dc.identifier.urihttp://hdl.handle.net/10754/597343en
dc.description.abstractFish ecologists have used geochemical values in otoliths to examine habitat use, migration, and population connectivity for decades. However, it remains difficult to determine an unambiguous dietary δ 13C signature from bulk analysis of otolith. Studies to date have focused on the aragonite component of otoliths with less attention paid to the organic fraction. We describe the application of compound-specific stable isotope analysis (SIA) to analyze amino acid (AA) δ 13C values from small amounts (<1 mg) of otolith powder. We examined δ 13C values of otolith and muscle AAs from a reef-associated snapper (Lutjanus ehrenbergii (Peters, 1869)) collected along a carbon isotope gradient (isoscape) from seagrass beds to coral reefs. Carbon isotope values in otolith and muscle samples were highly correlated within and among coastal habitats. Moreover, δ 13C values of otolith AAs provided a purely dietary record that avoided dilution from dissolved inorganic carbon. Otolith AAs served as a robust tracer of δ 13C values at the base of the food web, making compound-specific SIA a powerful tool for dietary reconstructions and tracking the movement of fishes across isoscapes.en
dc.description.sponsorshipThe authors thank M. Berumen for field assistance and the crew of Dream Divers, Jeddah, Saudi Arabia, for boat and dive operation assistance. The site map was created by C. Braun. This research was based on work supported by awards USA 00002 and KSA 00011 made by King Abdullah University of Science and Technology (KAUST). Additional funding was provided by Woods Hole Oceanographic Institution and an International Society for Reef Studies - Ocean Conservancy Coral Reef Fellowship. K. McMahon received support from the National Science Foundation Graduate Research Fellowship Program.en
dc.publisherCanadian Science Publishingen
dc.titleA new method to reconstruct fish diet and movement patterns from δ 13 C values in otolith amino acidsen
dc.typeArticleen
dc.identifier.journalCanadian Journal of Fisheries and Aquatic Sciencesen
dc.contributor.institutionWoods Hole Oceanographic Institution, Woods Hole, United Statesen
dc.contributor.institutionCarnegie Institution of Washington, Washington, United Statesen
dc.contributor.institutionBates College, Lewiston, United Statesen
kaust.grant.numberUSA 00002en
kaust.grant.numberKSA 00011en
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