A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement

Handle URI:
http://hdl.handle.net/10754/597339
Title:
A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement
Authors:
Lundahl, P. Johan; Kitts, Catherine C.; Nordén, Bengt
Abstract:
This article presents a new design of flow-orientation device for the study of bio-macromolecules, including DNA and protein complexes, as well as aggregates such as amyloid fibrils and liposome membranes, using Linear Dichroism (LD) spectroscopy. The design provides a number of technical advantages that should make the device inexpensive to manufacture, easier to use and more reliable than existing techniques. The degree of orientation achieved is of the same order of magnitude as that of the commonly used concentric cylinders Couette flow cell, however, since the device exploits a set of flat strain-free quartz plates, a number of problems associated with refraction and birefringence of light are eliminated, increasing the sensitivity and accuracy of measurement. The device provides similar shear rates to those of the Couette cell but is superior in that the shear rate is constant across the gap. Other major advantages of the design is the possibility to change parts and vary sample volume and path length easily and at a low cost. © 2011 The Royal Society of Chemistry.
Citation:
Lundahl PJ, Kitts CC, Nordén B (2011) A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement. Analyst 136: 3303. Available: http://dx.doi.org/10.1039/c0an00455c.
Publisher:
Royal Society of Chemistry (RSC)
Journal:
The Analyst
Issue Date:
2011
DOI:
10.1039/c0an00455c
PubMed ID:
21709867
Type:
Article
ISSN:
0003-2654; 1364-5528
Sponsors:
This work was funded by King Abdullah University of Science and Technology (KAUST). Hanna Rydberg is acknowledged for supplying the liposome samples.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorLundahl, P. Johanen
dc.contributor.authorKitts, Catherine C.en
dc.contributor.authorNordén, Bengten
dc.date.accessioned2016-02-25T12:31:02Zen
dc.date.available2016-02-25T12:31:02Zen
dc.date.issued2011en
dc.identifier.citationLundahl PJ, Kitts CC, Nordén B (2011) A new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurement. Analyst 136: 3303. Available: http://dx.doi.org/10.1039/c0an00455c.en
dc.identifier.issn0003-2654en
dc.identifier.issn1364-5528en
dc.identifier.pmid21709867en
dc.identifier.doi10.1039/c0an00455cen
dc.identifier.urihttp://hdl.handle.net/10754/597339en
dc.description.abstractThis article presents a new design of flow-orientation device for the study of bio-macromolecules, including DNA and protein complexes, as well as aggregates such as amyloid fibrils and liposome membranes, using Linear Dichroism (LD) spectroscopy. The design provides a number of technical advantages that should make the device inexpensive to manufacture, easier to use and more reliable than existing techniques. The degree of orientation achieved is of the same order of magnitude as that of the commonly used concentric cylinders Couette flow cell, however, since the device exploits a set of flat strain-free quartz plates, a number of problems associated with refraction and birefringence of light are eliminated, increasing the sensitivity and accuracy of measurement. The device provides similar shear rates to those of the Couette cell but is superior in that the shear rate is constant across the gap. Other major advantages of the design is the possibility to change parts and vary sample volume and path length easily and at a low cost. © 2011 The Royal Society of Chemistry.en
dc.description.sponsorshipThis work was funded by King Abdullah University of Science and Technology (KAUST). Hanna Rydberg is acknowledged for supplying the liposome samples.en
dc.publisherRoyal Society of Chemistry (RSC)en
dc.titleA new highly adaptable design of shear-flow device for orientation of macromolecules for Linear Dichroism (LD) measurementen
dc.typeArticleen
dc.identifier.journalThe Analysten
dc.contributor.institutionChalmers University of Technology, Göteborg, Swedenen

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