Combined Effect of Surface Nano-Topography and Delivery of Therapeutics on the Adhesion of Tumor Cells on Porous Silicon Substrates

Handle URI:
http://hdl.handle.net/10754/597082
Title:
Combined Effect of Surface Nano-Topography and Delivery of Therapeutics on the Adhesion of Tumor Cells on Porous Silicon Substrates
Authors:
De Vitis, S.; Coluccio, M.L.; Strumbo, G.; Malara, N.; Fanizzi, F.P.; De Pascali, S.A.; Perozziello, G.; Candeloro, P.; Di Fabrizio, Enzo ( 0000-0001-5886-4678 ) ; Gentile, F
Abstract:
Porous silicon is a nano material in which pores with different sizes, densities and depths are infiltrated in conventional silicon imparting it augmented properties including biodegradability, biocompatibility, photoluminescence. Here, we realized porous silicon substrates in which the pore size and the fractal dimension were varied over a significant range. We loaded the described substrates with a PtCl(O, O′ − acac)(DMSO) antitumor drug and determined its release profile as a function of pore size over time up to 15 days. We observed that the efficacy of delivery augments with the pore size moving from small (∼ 8nm, efficiency of delivery ∼ 0.2) to large (∼ 55nm, efficiency of delivery ∼ 0.7). Then, we verified the adhesion of MCF-7 breast cancer cells on the described substrates with and without the administration of the antitumor drug. This permitted to decouple and understand the coincidental effects of nano-topography and a controlled dosage of drugs on cell adhesion and growth. While large pore sizes guarantee elevated drug dosages, large fractal dimensions boost cell adhesion on a surface. For the particular case of tumor cells and the delivery of an anti-tumor drug, substrates with a small fractal dimension and large pore size hamper cell growth. The competition between nano-topography and a controlled dosage of drugs may either accelerate or block the adhesion of cells on a nanostructured surface, for applications in tissue engineering, regenerative medicine, personalized lab-on-a-chips, and the rational design of implantable drug delivery systems.
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Citation:
Combined Effect of Surface Nano-Topography and Delivery of Therapeutics on the Adhesion of Tumor Cells on Porous Silicon Substrates 2016 Microelectronic Engineering
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
23-Feb-2016
DOI:
10.1016/j.mee.2016.02.033
Type:
Article
ISSN:
01679317
Sponsors:
This work has been partially funded from the Italian Minister of Health (Project n. GR-2010-2320665).
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0167931716300764
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDe Vitis, S.en
dc.contributor.authorColuccio, M.L.en
dc.contributor.authorStrumbo, G.en
dc.contributor.authorMalara, N.en
dc.contributor.authorFanizzi, F.P.en
dc.contributor.authorDe Pascali, S.A.en
dc.contributor.authorPerozziello, G.en
dc.contributor.authorCandeloro, P.en
dc.contributor.authorDi Fabrizio, Enzoen
dc.contributor.authorGentile, Fen
dc.date.accessioned2016-02-24T09:31:02Zen
dc.date.available2016-02-24T09:31:02Zen
dc.date.issued2016-02-23en
dc.identifier.citationCombined Effect of Surface Nano-Topography and Delivery of Therapeutics on the Adhesion of Tumor Cells on Porous Silicon Substrates 2016 Microelectronic Engineeringen
dc.identifier.issn01679317en
dc.identifier.doi10.1016/j.mee.2016.02.033en
dc.identifier.urihttp://hdl.handle.net/10754/597082en
dc.description.abstractPorous silicon is a nano material in which pores with different sizes, densities and depths are infiltrated in conventional silicon imparting it augmented properties including biodegradability, biocompatibility, photoluminescence. Here, we realized porous silicon substrates in which the pore size and the fractal dimension were varied over a significant range. We loaded the described substrates with a PtCl(O, O′ − acac)(DMSO) antitumor drug and determined its release profile as a function of pore size over time up to 15 days. We observed that the efficacy of delivery augments with the pore size moving from small (∼ 8nm, efficiency of delivery ∼ 0.2) to large (∼ 55nm, efficiency of delivery ∼ 0.7). Then, we verified the adhesion of MCF-7 breast cancer cells on the described substrates with and without the administration of the antitumor drug. This permitted to decouple and understand the coincidental effects of nano-topography and a controlled dosage of drugs on cell adhesion and growth. While large pore sizes guarantee elevated drug dosages, large fractal dimensions boost cell adhesion on a surface. For the particular case of tumor cells and the delivery of an anti-tumor drug, substrates with a small fractal dimension and large pore size hamper cell growth. The competition between nano-topography and a controlled dosage of drugs may either accelerate or block the adhesion of cells on a nanostructured surface, for applications in tissue engineering, regenerative medicine, personalized lab-on-a-chips, and the rational design of implantable drug delivery systems.en
dc.description.sponsorshipThis work has been partially funded from the Italian Minister of Health (Project n. GR-2010-2320665).en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0167931716300764en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, 23 February 2016. DOI: 10.1016/j.mee.2016.02.033en
dc.subjectPorous Siliconen
dc.subjectNano-Topographyen
dc.subjectDrug Deliveryen
dc.subjectAnti-tumour drugen
dc.subjectCell Adhesionen
dc.titleCombined Effect of Surface Nano-Topography and Delivery of Therapeutics on the Adhesion of Tumor Cells on Porous Silicon Substratesen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalMicroelectronic Engineeringen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University Magna Graecia, Catanzaro, Italyen
dc.contributor.institutionDepartment of Environmental Biological Sciences and Technologies, University of Salento, Lecce, Italyen
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University Federico II, Naples, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorGentile, F.en
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