Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool

Handle URI:
http://hdl.handle.net/10754/597001
Title:
Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool
Authors:
Mohamed, Yasmine M.; Ghazy, Mohamed A.; Sayed, Ahmed; Ouf, Amged; El-Dorry, Hamza; Siam, Rania
Abstract:
The Red Sea Atlantis II brine pool is an extreme environment that displays multiple harsh conditions such as high temperature, high salinity and high concentrations of multiple, toxic heavy metals. The survival of microbes in such an environment by utilizing resistant enzymes makes them an excellent source of extremophilic enzymes. We constructed a fosmid metagenomic library using DNA isolated from the deepest and most secluded layer of this pool. We report the isolation and biochemical characterization of an unusual esterase: EstATII. EstATII is thermophilic (optimum temperature, 65 C), halotolerant (maintains its activity in up to 4.5â€...M NaCl) and maintains at least 60% of its activity in the presence of a wide spectrum of heavy metals. The combination of biochemical characteristics of the Red Sea Atlantis II brine pool esterase, i.e., halotolerance, thermophilicity and resistance to heavy metals, makes it a potentially useful biocatalyst.
Citation:
Mohamed YM, Ghazy MA, Sayed A, Ouf A, El-Dorry H, et al. (2013) Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool. Scientific Reports 3. Available: http://dx.doi.org/10.1038/srep03358.
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
28-Nov-2013
DOI:
10.1038/srep03358
PubMed ID:
24285146
Type:
Article
ISSN:
2045-2322
Sponsors:
This work was supported by King Abdullah University for Science and Technology Global Collaborative Partners (GCR) program. YMM was partly funded by an Al-Alfi Biotechnology Fellowship.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorMohamed, Yasmine M.en
dc.contributor.authorGhazy, Mohamed A.en
dc.contributor.authorSayed, Ahmeden
dc.contributor.authorOuf, Amgeden
dc.contributor.authorEl-Dorry, Hamzaen
dc.contributor.authorSiam, Raniaen
dc.date.accessioned2016-02-23T13:52:13Zen
dc.date.available2016-02-23T13:52:13Zen
dc.date.issued2013-11-28en
dc.identifier.citationMohamed YM, Ghazy MA, Sayed A, Ouf A, El-Dorry H, et al. (2013) Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Pool. Scientific Reports 3. Available: http://dx.doi.org/10.1038/srep03358.en
dc.identifier.issn2045-2322en
dc.identifier.pmid24285146en
dc.identifier.doi10.1038/srep03358en
dc.identifier.urihttp://hdl.handle.net/10754/597001en
dc.description.abstractThe Red Sea Atlantis II brine pool is an extreme environment that displays multiple harsh conditions such as high temperature, high salinity and high concentrations of multiple, toxic heavy metals. The survival of microbes in such an environment by utilizing resistant enzymes makes them an excellent source of extremophilic enzymes. We constructed a fosmid metagenomic library using DNA isolated from the deepest and most secluded layer of this pool. We report the isolation and biochemical characterization of an unusual esterase: EstATII. EstATII is thermophilic (optimum temperature, 65 C), halotolerant (maintains its activity in up to 4.5â€...M NaCl) and maintains at least 60% of its activity in the presence of a wide spectrum of heavy metals. The combination of biochemical characteristics of the Red Sea Atlantis II brine pool esterase, i.e., halotolerance, thermophilicity and resistance to heavy metals, makes it a potentially useful biocatalyst.en
dc.description.sponsorshipThis work was supported by King Abdullah University for Science and Technology Global Collaborative Partners (GCR) program. YMM was partly funded by an Al-Alfi Biotechnology Fellowship.en
dc.publisherNature Publishing Groupen
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.titleIsolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea Brine Poolen
dc.typeArticleen
dc.identifier.journalScientific Reportsen
dc.contributor.institutionAmerican University in Cairo, Cairo, Egypten
dc.contributor.institutionAmerican University in Cairo, Cairo, Egypten

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