Functional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time

Handle URI:
http://hdl.handle.net/10754/596898
Title:
Functional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time
Authors:
Hwang, Yoon-Hyung; Kim, SoonKap; Lee, Keh Chien; Chung, Young Soo; Lee, Jeong Hwan; Kim, Jeong-Kook
Abstract:
Plant NUCLEAR FACTOR Y (NF-Y) transcription factors play important roles in plant development and abiotic stress. In Arabidopsis thaliana, two NF-YB (AtNF-YB2 and AtNF-YB3) and five NF-YC (AtNF-YC1, AtNF-YC2, AtNF-YC3, AtNF-YC4, and AtNF-YC9) genes regulate photoperiodic flowering by interacting with other AtNF-Y subunit proteins. Three rice NF-YB (OsNF-YB8, OsNF-YB10, and OsNF-YB11) and five rice OsNF-YC (OsNF-YC1, OsNF-YC2, OsNF-YC4, OsNF-YC6, and OsNF-YC7) genes are clustered with two AtNF-YB and five AtNF-YC genes, respectively. To investigate the functional conservation of these NF-YB and NF-YC genes in rice and Arabidopsis, we analyzed the flowering phenotypes of transgenic plants overexpressing the respective OsNF-YB and OsNF-YC genes in Arabidopsis mutants. Overexpression of OsNF-YB8/10/11 and OsNF-YC2 complemented the late flowering phenotype of Arabidopsis nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants, respectively. The rescued phenotype of 35S::OsNF-YC2 nf-yc3 nf-yc4 nf-yc9 plants was attributed to the upregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). In vitro and in planta protein–protein analyses revealed that OsNF-YB8/10/11 and OsNF-YC1/2/4/6/7 interact with AtNF-YC3/4/9 and AtNF-YB2/3, respectively. Our data indicate that some OsNF-YB and OsNF-YC genes are functional equivalents of AtNF-YB2/3 and AtNF-YC3/4/9 genes, respectively, and suggest functional conservation of Arabidopsis and rice NF-Y genes in the control of flowering time.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Desert Agriculture Initiative
Citation:
Functional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time 2016 Plant Cell Reports
Publisher:
Springer Science + Business Media
Journal:
Plant Cell Reports
Issue Date:
11-Jan-2016
DOI:
10.1007/s00299-015-1927-1
Type:
Article
ISSN:
0721-7714; 1432-203X
Sponsors:
We thank Prof. Ben F. Holt III for providing nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants and other mutants not used in this study. This work was supported by the Next-Generation BioGreen 21 Program (PJ007978), Rural Development Administration, Republic of Korea and a Korea University Grant (to J.-K. Kim), Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A4A0101941 to J.H. Lee and 2014R1A1A2055796 to J.-K. Kim), and by the BK 21 program (to S.-K. Kim).
Additional Links:
http://link.springer.com/10.1007/s00299-015-1927-1
Appears in Collections:
Articles; Desert Agriculture Initiative; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorHwang, Yoon-Hyungen
dc.contributor.authorKim, SoonKapen
dc.contributor.authorLee, Keh Chienen
dc.contributor.authorChung, Young Sooen
dc.contributor.authorLee, Jeong Hwanen
dc.contributor.authorKim, Jeong-Kooken
dc.date.accessioned2016-02-22T09:46:44Zen
dc.date.available2016-02-22T09:46:44Zen
dc.date.issued2016-01-11en
dc.identifier.citationFunctional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time 2016 Plant Cell Reportsen
dc.identifier.issn0721-7714en
dc.identifier.issn1432-203Xen
dc.identifier.doi10.1007/s00299-015-1927-1en
dc.identifier.urihttp://hdl.handle.net/10754/596898en
dc.description.abstractPlant NUCLEAR FACTOR Y (NF-Y) transcription factors play important roles in plant development and abiotic stress. In Arabidopsis thaliana, two NF-YB (AtNF-YB2 and AtNF-YB3) and five NF-YC (AtNF-YC1, AtNF-YC2, AtNF-YC3, AtNF-YC4, and AtNF-YC9) genes regulate photoperiodic flowering by interacting with other AtNF-Y subunit proteins. Three rice NF-YB (OsNF-YB8, OsNF-YB10, and OsNF-YB11) and five rice OsNF-YC (OsNF-YC1, OsNF-YC2, OsNF-YC4, OsNF-YC6, and OsNF-YC7) genes are clustered with two AtNF-YB and five AtNF-YC genes, respectively. To investigate the functional conservation of these NF-YB and NF-YC genes in rice and Arabidopsis, we analyzed the flowering phenotypes of transgenic plants overexpressing the respective OsNF-YB and OsNF-YC genes in Arabidopsis mutants. Overexpression of OsNF-YB8/10/11 and OsNF-YC2 complemented the late flowering phenotype of Arabidopsis nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants, respectively. The rescued phenotype of 35S::OsNF-YC2 nf-yc3 nf-yc4 nf-yc9 plants was attributed to the upregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). In vitro and in planta protein–protein analyses revealed that OsNF-YB8/10/11 and OsNF-YC1/2/4/6/7 interact with AtNF-YC3/4/9 and AtNF-YB2/3, respectively. Our data indicate that some OsNF-YB and OsNF-YC genes are functional equivalents of AtNF-YB2/3 and AtNF-YC3/4/9 genes, respectively, and suggest functional conservation of Arabidopsis and rice NF-Y genes in the control of flowering time.en
dc.description.sponsorshipWe thank Prof. Ben F. Holt III for providing nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants and other mutants not used in this study. This work was supported by the Next-Generation BioGreen 21 Program (PJ007978), Rural Development Administration, Republic of Korea and a Korea University Grant (to J.-K. Kim), Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A4A0101941 to J.H. Lee and 2014R1A1A2055796 to J.-K. Kim), and by the BK 21 program (to S.-K. Kim).en
dc.language.isoenen
dc.publisherSpringer Science + Business Mediaen
dc.relation.urlhttp://link.springer.com/10.1007/s00299-015-1927-1en
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/s00299-015-1927-1en
dc.subjectAtNF-YB/YCen
dc.subjectFlowering timeen
dc.subjectNUCLEAR FACTOR Y transcription factorsen
dc.subjectOsNF-YB/YCen
dc.subjectProtein–protein interactionen
dc.titleFunctional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering timeen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentDesert Agriculture Initiativeen
dc.identifier.journalPlant Cell Reportsen
dc.eprint.versionPost-printen
dc.contributor.institutionDivision of Life Sciences, Korea University, Anam-dong 5 ga, Seongbuk-gu, Seoul, 136-701, Republic of Koreaen
dc.contributor.institutionDepartment of Bioresource Engineering, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul, 143-747, Republic of Koreaen
dc.contributor.institutionDepartment of Genetic Engineering, Dong-A University, Busan, 604-714, Republic of Koreaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKim, SoonKapen
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