Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.

Handle URI:
http://hdl.handle.net/10754/596843
Title:
Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.
Authors:
Balk, Melike; Heilig, Hans G H J; van Eekert, Miriam H A; Stams, Alfons J M; Rijpstra, Irene C; Sinninghe-Damsté, Jaap S; de Vos, Willem M; Kengen, Servé W M
Abstract:
A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.
Citation:
Balk M, Heilig HGHJ, van Eekert MHA, Stams AJM, Rijpstra IC, et al. (2009) Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey. Extremophiles 13: 885–894. Available: http://dx.doi.org/10.1007/s00792-009-0276-9.
Publisher:
Springer Nature
Journal:
Extremophiles
KAUST Grant Number:
KUK-C1-017-12
Issue Date:
23-Aug-2009
DOI:
10.1007/s00792-009-0276-9
PubMed ID:
19701714
PubMed Central ID:
PMC2767516
Type:
Article
ISSN:
1431-0651; 1433-4909
Sponsors:
This work was supported by the INTAS and Darwin Centre for Biogeology of Netherlands Organization for Scientific Research (NWO) and was also partly supported by Award No KUK-C1-017-12, made by King Abdullah University of Science and Technology (KAUST).
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DC FieldValue Language
dc.contributor.authorBalk, Melikeen
dc.contributor.authorHeilig, Hans G H Jen
dc.contributor.authorvan Eekert, Miriam H Aen
dc.contributor.authorStams, Alfons J Men
dc.contributor.authorRijpstra, Irene Cen
dc.contributor.authorSinninghe-Damsté, Jaap Sen
dc.contributor.authorde Vos, Willem Men
dc.contributor.authorKengen, Servé W Men
dc.date.accessioned2016-02-21T09:35:13Zen
dc.date.available2016-02-21T09:35:13Zen
dc.date.issued2009-08-23en
dc.identifier.citationBalk M, Heilig HGHJ, van Eekert MHA, Stams AJM, Rijpstra IC, et al. (2009) Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey. Extremophiles 13: 885–894. Available: http://dx.doi.org/10.1007/s00792-009-0276-9.en
dc.identifier.issn1431-0651en
dc.identifier.issn1433-4909en
dc.identifier.pmid19701714en
dc.identifier.doi10.1007/s00792-009-0276-9en
dc.identifier.urihttp://hdl.handle.net/10754/596843en
dc.description.abstractA novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4-0.6 microm in diameter and 3.5-10 microm in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.en
dc.description.sponsorshipThis work was supported by the INTAS and Darwin Centre for Biogeology of Netherlands Organization for Scientific Research (NWO) and was also partly supported by Award No KUK-C1-017-12, made by King Abdullah University of Science and Technology (KAUST).en
dc.publisherSpringer Natureen
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.en
dc.titleIsolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey.en
dc.typeArticleen
dc.identifier.journalExtremophilesen
dc.identifier.pmcidPMC2767516en
dc.contributor.institutionWageningen University and Research Centre, Wageningen, Netherlandsen
dc.contributor.institutionRoyal Netherlands Institute for Sea Research - NIOZ, 't Horntje, Netherlandsen
dc.contributor.institutionNIOO Centre for Limnology - CL, Nieuwersluis, Netherlandsen
kaust.grant.numberKUK-C1-017-12en
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