Effect of methanogenic substrates on anaerobic oxidation of methane and sulfate reduction by an anaerobic methanotrophic enrichment.

Handle URI:
http://hdl.handle.net/10754/596840
Title:
Effect of methanogenic substrates on anaerobic oxidation of methane and sulfate reduction by an anaerobic methanotrophic enrichment.
Authors:
Meulepas, Roel J W; Jagersma, Christian G; Khadem, Ahmad F; Stams, Alfons J M; Lens, Piet N L
Abstract:
Anaerobic oxidation of methane (AOM) coupled to sulfate reduction (SR) is assumed to be a syntrophic process, in which methanotrophic archaea produce an interspecies electron carrier (IEC), which is subsequently utilized by sulfate-reducing bacteria. In this paper, six methanogenic substrates are tested as candidate-IECs by assessing their effect on AOM and SR by an anaerobic methanotrophic enrichment. The presence of acetate, formate or hydrogen enhanced SR, but did not inhibit AOM, nor did these substrates trigger methanogenesis. Carbon monoxide also enhanced SR but slightly inhibited AOM. Methanol did not enhance SR nor did it inhibit AOM, and methanethiol inhibited both SR and AOM completely. Subsequently, it was calculated at which candidate-IEC concentrations no more Gibbs free energy can be conserved from their production from methane at the applied conditions. These concentrations were at least 1,000 times lower can the final candidate-IEC concentration in the bulk liquid. Therefore, the tested candidate-IECs could not have been produced from methane during the incubations. Hence, acetate, formate, methanol, carbon monoxide, and hydrogen can be excluded as sole IEC in AOM coupled to SR. Methanethiol did inhibit AOM and can therefore not be excluded as IEC by this study.
Citation:
Meulepas RJW, Jagersma CG, Khadem AF, Stams AJM, Lens PNL (2010) Effect of methanogenic substrates on anaerobic oxidation of methane and sulfate reduction by an anaerobic methanotrophic enrichment. Applied Microbiology and Biotechnology 87: 1499–1506. Available: http://dx.doi.org/10.1007/s00253-010-2597-0.
Publisher:
Springer Nature
Journal:
Applied Microbiology and Biotechnology
Issue Date:
6-May-2010
DOI:
10.1007/s00253-010-2597-0
PubMed ID:
20445975
PubMed Central ID:
PMC2892604
Type:
Article
ISSN:
0175-7598; 1432-0614
Sponsors:
This work was part the Anaerobic Methane Oxidation for Sulfate Reduction project supported by the Dutch ministries of Economical affairs, Education, culture and science, and Environment and special planning as part their EET program and was cofunded by King Abdullah University of Science and Technology through the SOWACOR project.
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DC FieldValue Language
dc.contributor.authorMeulepas, Roel J Wen
dc.contributor.authorJagersma, Christian Gen
dc.contributor.authorKhadem, Ahmad Fen
dc.contributor.authorStams, Alfons J Men
dc.contributor.authorLens, Piet N Len
dc.date.accessioned2016-02-21T09:35:09Zen
dc.date.available2016-02-21T09:35:09Zen
dc.date.issued2010-05-06en
dc.identifier.citationMeulepas RJW, Jagersma CG, Khadem AF, Stams AJM, Lens PNL (2010) Effect of methanogenic substrates on anaerobic oxidation of methane and sulfate reduction by an anaerobic methanotrophic enrichment. Applied Microbiology and Biotechnology 87: 1499–1506. Available: http://dx.doi.org/10.1007/s00253-010-2597-0.en
dc.identifier.issn0175-7598en
dc.identifier.issn1432-0614en
dc.identifier.pmid20445975en
dc.identifier.doi10.1007/s00253-010-2597-0en
dc.identifier.urihttp://hdl.handle.net/10754/596840en
dc.description.abstractAnaerobic oxidation of methane (AOM) coupled to sulfate reduction (SR) is assumed to be a syntrophic process, in which methanotrophic archaea produce an interspecies electron carrier (IEC), which is subsequently utilized by sulfate-reducing bacteria. In this paper, six methanogenic substrates are tested as candidate-IECs by assessing their effect on AOM and SR by an anaerobic methanotrophic enrichment. The presence of acetate, formate or hydrogen enhanced SR, but did not inhibit AOM, nor did these substrates trigger methanogenesis. Carbon monoxide also enhanced SR but slightly inhibited AOM. Methanol did not enhance SR nor did it inhibit AOM, and methanethiol inhibited both SR and AOM completely. Subsequently, it was calculated at which candidate-IEC concentrations no more Gibbs free energy can be conserved from their production from methane at the applied conditions. These concentrations were at least 1,000 times lower can the final candidate-IEC concentration in the bulk liquid. Therefore, the tested candidate-IECs could not have been produced from methane during the incubations. Hence, acetate, formate, methanol, carbon monoxide, and hydrogen can be excluded as sole IEC in AOM coupled to SR. Methanethiol did inhibit AOM and can therefore not be excluded as IEC by this study.en
dc.description.sponsorshipThis work was part the Anaerobic Methane Oxidation for Sulfate Reduction project supported by the Dutch ministries of Economical affairs, Education, culture and science, and Environment and special planning as part their EET program and was cofunded by King Abdullah University of Science and Technology through the SOWACOR project.en
dc.publisherSpringer Natureen
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.en
dc.titleEffect of methanogenic substrates on anaerobic oxidation of methane and sulfate reduction by an anaerobic methanotrophic enrichment.en
dc.typeArticleen
dc.identifier.journalApplied Microbiology and Biotechnologyen
dc.identifier.pmcidPMC2892604en
dc.contributor.institutionUNESCO-IHE Institute for Water Education, Delft, Netherlandsen
dc.contributor.institutionWageningen University and Research Centre, Wageningen, Netherlandsen
dc.contributor.institutionWageningen University and Research Centre, Wageningen, Netherlandsen
dc.contributor.institutionRadboud University Nijmegen, Nijmegen, Netherlandsen

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