Transcriptomic analysis of neuropeptides and peptide hormones in the barnacle Balanus amphitrite: evidence of roles in larval settlement.

Handle URI:
http://hdl.handle.net/10754/596830
Title:
Transcriptomic analysis of neuropeptides and peptide hormones in the barnacle Balanus amphitrite: evidence of roles in larval settlement.
Authors:
Yan, Xing-Cheng; Chen, Zhang-Fan; Sun, Jin; Matsumura, Kiyotaka; Wu, Rudolf S S; Qian, Pei-Yuan
Abstract:
The barnacle Balanus amphitrite is a globally distributed marine crustacean and has been used as a model species for intertidal ecology and biofouling studies. Its life cycle consists of seven planktonic larval stages followed by a sessile juvenile/adult stage. The transitional processes between larval stages and juveniles are crucial for barnacle development and recruitment. Although some studies have been conducted on the neuroanatomy and neuroactive substances of the barnacle, a comprehensive understanding of neuropeptides and peptide hormones remains lacking. To better characterize barnacle neuropeptidome and its potential roles in larval settlement, an in silico identification of putative transcripts encoding neuropeptides/peptide hormones was performed, based on transcriptome of the barnacle B. amphitrite that has been recently sequenced. Potential cleavage sites andstructure of mature peptides were predicted through homology search of known arthropod peptides. In total, 16 neuropeptide families/subfamilies were predicted from the barnacle transcriptome, and 14 of them were confirmed as genuine neuropeptides by Rapid Amplification of cDNA Ends. Analysis of peptide precursor structures and mature sequences showed that some neuropeptides of B. amphitrite are novel isoforms and shared similar characteristics with their homologs from insects. The expression profiling of predicted neuropeptide genes revealed that pigment dispersing hormone, SIFamide, calcitonin, and B-type allatostatin had the highest expression level in cypris stage, while tachykinin-related peptide was down regulated in both cyprids and juveniles. Furthermore, an inhibitor of proprotein convertase related to peptide maturation effectively delayed larval metamorphosis. Combination of real-time PCR results and bioassay indicated that certain neuropeptides may play an important role in cypris settlement. Overall, new insight into neuropeptides/peptide hormones characterized in this study shall provide a platform for unraveling peptidergic control of barnacle larval behavior and settlement process.
Citation:
Yan X-C, Chen Z-F, Sun J, Matsumura K, Wu RSS, et al. (2012) Transcriptomic Analysis of Neuropeptides and Peptide Hormones in the Barnacle Balanus amphitrite: Evidence of Roles in Larval Settlement. PLoS ONE 7: e46513. Available: http://dx.doi.org/10.1371/journal.pone.0046513.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
KAUST Grant Number:
SA-C0040/UK-C001
Issue Date:
2-Oct-2012
DOI:
10.1371/journal.pone.0046513
PubMed ID:
23056329
PubMed Central ID:
PMC3462748
Type:
Article
ISSN:
1932-6203
Sponsors:
This work was supported by a research grant (DY125-15-T-02) from China Ocean Mineral Resources Research and Development Association and an Award (SA-C0040/UK-C001) from King Abdullah University of Science and Technology (KAUST) to PY Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Full metadata record

DC FieldValue Language
dc.contributor.authorYan, Xing-Chengen
dc.contributor.authorChen, Zhang-Fanen
dc.contributor.authorSun, Jinen
dc.contributor.authorMatsumura, Kiyotakaen
dc.contributor.authorWu, Rudolf S Sen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2016-02-21T08:51:29Zen
dc.date.available2016-02-21T08:51:29Zen
dc.date.issued2012-10-02en
dc.identifier.citationYan X-C, Chen Z-F, Sun J, Matsumura K, Wu RSS, et al. (2012) Transcriptomic Analysis of Neuropeptides and Peptide Hormones in the Barnacle Balanus amphitrite: Evidence of Roles in Larval Settlement. PLoS ONE 7: e46513. Available: http://dx.doi.org/10.1371/journal.pone.0046513.en
dc.identifier.issn1932-6203en
dc.identifier.pmid23056329en
dc.identifier.doi10.1371/journal.pone.0046513en
dc.identifier.urihttp://hdl.handle.net/10754/596830en
dc.description.abstractThe barnacle Balanus amphitrite is a globally distributed marine crustacean and has been used as a model species for intertidal ecology and biofouling studies. Its life cycle consists of seven planktonic larval stages followed by a sessile juvenile/adult stage. The transitional processes between larval stages and juveniles are crucial for barnacle development and recruitment. Although some studies have been conducted on the neuroanatomy and neuroactive substances of the barnacle, a comprehensive understanding of neuropeptides and peptide hormones remains lacking. To better characterize barnacle neuropeptidome and its potential roles in larval settlement, an in silico identification of putative transcripts encoding neuropeptides/peptide hormones was performed, based on transcriptome of the barnacle B. amphitrite that has been recently sequenced. Potential cleavage sites andstructure of mature peptides were predicted through homology search of known arthropod peptides. In total, 16 neuropeptide families/subfamilies were predicted from the barnacle transcriptome, and 14 of them were confirmed as genuine neuropeptides by Rapid Amplification of cDNA Ends. Analysis of peptide precursor structures and mature sequences showed that some neuropeptides of B. amphitrite are novel isoforms and shared similar characteristics with their homologs from insects. The expression profiling of predicted neuropeptide genes revealed that pigment dispersing hormone, SIFamide, calcitonin, and B-type allatostatin had the highest expression level in cypris stage, while tachykinin-related peptide was down regulated in both cyprids and juveniles. Furthermore, an inhibitor of proprotein convertase related to peptide maturation effectively delayed larval metamorphosis. Combination of real-time PCR results and bioassay indicated that certain neuropeptides may play an important role in cypris settlement. Overall, new insight into neuropeptides/peptide hormones characterized in this study shall provide a platform for unraveling peptidergic control of barnacle larval behavior and settlement process.en
dc.description.sponsorshipThis work was supported by a research grant (DY125-15-T-02) from China Ocean Mineral Resources Research and Development Association and an Award (SA-C0040/UK-C001) from King Abdullah University of Science and Technology (KAUST) to PY Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.publisherPublic Library of Science (PLoS)en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subject.meshTranscriptomeen
dc.titleTranscriptomic analysis of neuropeptides and peptide hormones in the barnacle Balanus amphitrite: evidence of roles in larval settlement.en
dc.typeArticleen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3462748en
dc.contributor.institutionHong Kong University of Science and Technology, Hong Kong, Chinaen
dc.contributor.institutionHong Kong Baptist University, Hong Kong, Chinaen
dc.contributor.institutionThe University of Hong Kong, Pokfulam, Hong Kongen
kaust.grant.numberSA-C0040/UK-C001en

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