The association of heavy and light chain variable domains in antibodies: implications for antigen specificity.

Handle URI:
http://hdl.handle.net/10754/596822
Title:
The association of heavy and light chain variable domains in antibodies: implications for antigen specificity.
Authors:
Chailyan, Anna; Marcatili, Paolo; Tramontano, Anna
Abstract:
The antigen-binding site of immunoglobulins is formed by six regions, three from the light and three from the heavy chain variable domains, which, on association of the two chains, form the conventional antigen-binding site of the antibody. The mode of interaction between the heavy and light chain variable domains affects the relative position of the antigen-binding loops and therefore has an effect on the overall conformation of the binding site. In this article, we analyze the structure of the interface between the heavy and light chain variable domains and show that there are essentially two different modes for their interaction that can be identified by the presence of key amino acids in specific positions of the antibody sequences. We also show that the different packing modes are related to the type of recognized antigen.
Citation:
Chailyan A, Marcatili P, Tramontano A (2011) The association of heavy and light chain variable domains in antibodies: implications for antigen specificity. FEBS Journal 278: 2858–2866. Available: http://dx.doi.org/10.1111/j.1742-4658.2011.08207.x.
Publisher:
Wiley-Blackwell
Journal:
FEBS Journal
KAUST Grant Number:
KUK-I1-012-43
Issue Date:
28-Jun-2011
DOI:
10.1111/j.1742-4658.2011.08207.x
PubMed ID:
21651726
PubMed Central ID:
PMC3562479
Type:
Article
ISSN:
1742-464X
Sponsors:
This work was partially supported by Award No. KUK-I1-012-43 made by the King Abdullah University of Science and Technology (KAUST), by Fondazione Roma and by the Italian Ministry of Health, contract no. onc_ord 25/07, FIRB ITALBIONET and PROTEOMICA.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorChailyan, Annaen
dc.contributor.authorMarcatili, Paoloen
dc.contributor.authorTramontano, Annaen
dc.date.accessioned2016-02-21T08:51:20Zen
dc.date.available2016-02-21T08:51:20Zen
dc.date.issued2011-06-28en
dc.identifier.citationChailyan A, Marcatili P, Tramontano A (2011) The association of heavy and light chain variable domains in antibodies: implications for antigen specificity. FEBS Journal 278: 2858–2866. Available: http://dx.doi.org/10.1111/j.1742-4658.2011.08207.x.en
dc.identifier.issn1742-464Xen
dc.identifier.pmid21651726en
dc.identifier.doi10.1111/j.1742-4658.2011.08207.xen
dc.identifier.urihttp://hdl.handle.net/10754/596822en
dc.description.abstractThe antigen-binding site of immunoglobulins is formed by six regions, three from the light and three from the heavy chain variable domains, which, on association of the two chains, form the conventional antigen-binding site of the antibody. The mode of interaction between the heavy and light chain variable domains affects the relative position of the antigen-binding loops and therefore has an effect on the overall conformation of the binding site. In this article, we analyze the structure of the interface between the heavy and light chain variable domains and show that there are essentially two different modes for their interaction that can be identified by the presence of key amino acids in specific positions of the antibody sequences. We also show that the different packing modes are related to the type of recognized antigen.en
dc.description.sponsorshipThis work was partially supported by Award No. KUK-I1-012-43 made by the King Abdullah University of Science and Technology (KAUST), by Fondazione Roma and by the Italian Ministry of Health, contract no. onc_ord 25/07, FIRB ITALBIONET and PROTEOMICA.en
dc.publisherWiley-Blackwellen
dc.rightsRe-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en
dc.titleThe association of heavy and light chain variable domains in antibodies: implications for antigen specificity.en
dc.typeArticleen
dc.identifier.journalFEBS Journalen
dc.identifier.pmcidPMC3562479en
dc.contributor.institutionUniversita degli Studi di Roma La Sapienza, Rome, Italyen
dc.contributor.institutionUniversita degli Studi di Roma La Sapienza, Rome, Italyen
kaust.grant.numberKUK-I1-012-43en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.