Handle URI:
http://hdl.handle.net/10754/596821
Title:
Tabhu: tools for antibody humanization.
Authors:
Olimpieri, Pier Paolo; Marcatili, Paolo; Tramontano, Anna
Abstract:
SUMMARY: Antibodies are rapidly becoming essential tools in the clinical practice, given their ability to recognize their cognate antigens with high specificity and affinity, and a high yield at reasonable costs in model animals. Unfortunately, when administered to human patients, xenogeneic antibodies can elicit unwanted and dangerous immunogenic responses. Antibody humanization methods are designed to produce molecules with a better safety profile still maintaining their ability to bind the antigen. This can be accomplished by grafting the non-human regions determining the antigen specificity into a suitable human template. Unfortunately, this procedure may results in a partial or complete loss of affinity of the grafted molecule that can be restored by back-mutating some of the residues of human origin to the corresponding murine ones. This trial-and-error procedure is hard and involves expensive and time-consuming experiments. Here we present tools for antibody humanization (Tabhu) a web server for antibody humanization. Tabhu includes tools for human template selection, grafting, back-mutation evaluation, antibody modelling and structural analysis, helping the user in all the critical steps of the humanization experiment protocol. AVAILABILITY: http://www.biocomputing.it/tabhu CONTACT: anna.tramontano@uniroma1.it, pierpaolo.olimpieri@uniroma1.it SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
Citation:
Olimpieri PP, Marcatili P, Tramontano A (2014) Tabhu: tools for antibody humanization. Bioinformatics 31: 434–435. Available: http://dx.doi.org/10.1093/bioinformatics/btu667.
Publisher:
Oxford University Press (OUP)
Journal:
Bioinformatics
KAUST Grant Number:
KUK-I1-012-43
Issue Date:
9-Oct-2014
DOI:
10.1093/bioinformatics/btu667
PubMed ID:
25304777
PubMed Central ID:
PMC4308665
Type:
Article
ISSN:
1367-4803; 1460-2059
Sponsors:
KAUST Award No. KUK-I1-012-43 made by King Abdullah University of Science and Technology (KAUST), FIRB RBIN06E9Z8_005, PRIN 20108XYHJS and the Epigenomics Flagship Project - EPIGEN.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorOlimpieri, Pier Paoloen
dc.contributor.authorMarcatili, Paoloen
dc.contributor.authorTramontano, Annaen
dc.date.accessioned2016-02-21T08:51:19Zen
dc.date.available2016-02-21T08:51:19Zen
dc.date.issued2014-10-09en
dc.identifier.citationOlimpieri PP, Marcatili P, Tramontano A (2014) Tabhu: tools for antibody humanization. Bioinformatics 31: 434–435. Available: http://dx.doi.org/10.1093/bioinformatics/btu667.en
dc.identifier.issn1367-4803en
dc.identifier.issn1460-2059en
dc.identifier.pmid25304777en
dc.identifier.doi10.1093/bioinformatics/btu667en
dc.identifier.urihttp://hdl.handle.net/10754/596821en
dc.description.abstractSUMMARY: Antibodies are rapidly becoming essential tools in the clinical practice, given their ability to recognize their cognate antigens with high specificity and affinity, and a high yield at reasonable costs in model animals. Unfortunately, when administered to human patients, xenogeneic antibodies can elicit unwanted and dangerous immunogenic responses. Antibody humanization methods are designed to produce molecules with a better safety profile still maintaining their ability to bind the antigen. This can be accomplished by grafting the non-human regions determining the antigen specificity into a suitable human template. Unfortunately, this procedure may results in a partial or complete loss of affinity of the grafted molecule that can be restored by back-mutating some of the residues of human origin to the corresponding murine ones. This trial-and-error procedure is hard and involves expensive and time-consuming experiments. Here we present tools for antibody humanization (Tabhu) a web server for antibody humanization. Tabhu includes tools for human template selection, grafting, back-mutation evaluation, antibody modelling and structural analysis, helping the user in all the critical steps of the humanization experiment protocol. AVAILABILITY: http://www.biocomputing.it/tabhu CONTACT: anna.tramontano@uniroma1.it, pierpaolo.olimpieri@uniroma1.it SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.en
dc.description.sponsorshipKAUST Award No. KUK-I1-012-43 made by King Abdullah University of Science and Technology (KAUST), FIRB RBIN06E9Z8_005, PRIN 20108XYHJS and the Epigenomics Flagship Project - EPIGEN.en
dc.publisherOxford University Press (OUP)en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subject.meshSoftwareen
dc.titleTabhu: tools for antibody humanization.en
dc.typeArticleen
dc.identifier.journalBioinformaticsen
dc.identifier.pmcidPMC4308665en
dc.contributor.institutionDepartment of Physics, Sapienza University, Rome 00185, Italy, Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Anker Engelunds Vej 1, 2800 Lyngby, Denmark and Istituto Pasteur-Fondazione Cenci Bolognetti, Rome 00185, Italy.en
dc.contributor.institutionDepartment of Physics, Sapienza University, Rome 00185, Italy, Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Anker Engelunds Vej 1, 2800 Lyngby, Denmark and Istituto Pasteur-Fondazione Cenci Bolognetti, Rome 00185, Italy Department of Physics, Sapienza University, Rome 00185, Italy, Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Anker Engelunds Vej 1, 2800 Lyngby, Denmark and Istituto Pasteur-Fondazione Cenci Bolognetti, Rome 00185, Italy.en
kaust.grant.numberKUK-I1-012-43en

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