Swi5-Sfr1 protein stimulates Rad51-mediated DNA strand exchange reaction through organization of DNA bases in the presynaptic filament.

Handle URI:
http://hdl.handle.net/10754/596820
Title:
Swi5-Sfr1 protein stimulates Rad51-mediated DNA strand exchange reaction through organization of DNA bases in the presynaptic filament.
Authors:
Fornander, Louise H; Renodon-Cornière, Axelle; Kuwabara, Naoyuki; Ito, Kentaro; Tsutsui, Yasuhiro; Shimizu, Toshiyuki; Iwasaki, Hiroshi; Nordén, Bengt; Takahashi, Masayuki
Abstract:
The Swi5-Sfr1 heterodimer protein stimulates the Rad51-promoted DNA strand exchange reaction, a crucial step in homologous recombination. To clarify how this accessory protein acts on the strand exchange reaction, we have analyzed how the structure of the primary reaction intermediate, the Rad51/single-stranded DNA (ssDNA) complex filament formed in the presence of ATP, is affected by Swi5-Sfr1. Using flow linear dichroism spectroscopy, we observe that the nucleobases of the ssDNA are more perpendicularly aligned to the filament axis in the presence of Swi5-Sfr1, whereas the bases are more randomly oriented in the absence of Swi5-Sfr1. When using a modified version of the natural protein where the N-terminal part of Sfr1 is deleted, which has no affinity for DNA but maintained ability to stimulate the strand exchange reaction, we still observe the improved perpendicular DNA base orientation. This indicates that Swi5-Sfr1 exerts its activating effect through interaction with the Rad51 filament mainly and not with the DNA. We propose that the role of a coplanar alignment of nucleobases induced by Swi5-Sfr1 in the presynaptic Rad51/ssDNA complex is to facilitate the critical matching with an invading double-stranded DNA, hence stimulating the strand exchange reaction.
Citation:
Fornander LH, Renodon-Corniere A, Kuwabara N, Ito K, Tsutsui Y, et al. (2013) Swi5-Sfr1 protein stimulates Rad51-mediated DNA strand exchange reaction through organization of DNA bases in the presynaptic filament. Nucleic Acids Research 42: 2358–2365. Available: http://dx.doi.org/10.1093/nar/gkt1257.
Publisher:
Oxford University Press (OUP)
Journal:
Nucleic Acids Research
KAUST Grant Number:
KUK-11-008-23
Issue Date:
3-Dec-2013
DOI:
10.1093/nar/gkt1257
PubMed ID:
24304898
PubMed Central ID:
PMC3936755
Type:
Article
ISSN:
0305-1048; 1362-4962
Sponsors:
King Abdullah University of Science and Technology Grant [KUK-11-008-23 to L.F.]; the Agence Nationale de la Recherche Grant [ANR-2010-BLAN-1013 DynRec to A.R-C. and M.T.]; the European Research Council [ERC-2008-AdG 227700 to B.N.]; Ministry of Education, Culture, Sports, Science and Technology (MEXT) Japan [22125003 to H.I.]; and Japan Society for the Promotion of Science (JSPS) KAKENHI Grant [23770105 to N.K.]; The visit of M.T. to Japan was supported by JSPS Short-term Fellowship and that of N.K. to France by JSPS Institutional Program for Young Researcher Overseas Visits. Funding for open access charge: The Agence Nationale de la Recherche [ANR-2010-BLAN-1013].
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorFornander, Louise Hen
dc.contributor.authorRenodon-Cornière, Axelleen
dc.contributor.authorKuwabara, Naoyukien
dc.contributor.authorIto, Kentaroen
dc.contributor.authorTsutsui, Yasuhiroen
dc.contributor.authorShimizu, Toshiyukien
dc.contributor.authorIwasaki, Hiroshien
dc.contributor.authorNordén, Bengten
dc.contributor.authorTakahashi, Masayukien
dc.date.accessioned2016-02-21T08:51:18Zen
dc.date.available2016-02-21T08:51:18Zen
dc.date.issued2013-12-03en
dc.identifier.citationFornander LH, Renodon-Corniere A, Kuwabara N, Ito K, Tsutsui Y, et al. (2013) Swi5-Sfr1 protein stimulates Rad51-mediated DNA strand exchange reaction through organization of DNA bases in the presynaptic filament. Nucleic Acids Research 42: 2358–2365. Available: http://dx.doi.org/10.1093/nar/gkt1257.en
dc.identifier.issn0305-1048en
dc.identifier.issn1362-4962en
dc.identifier.pmid24304898en
dc.identifier.doi10.1093/nar/gkt1257en
dc.identifier.urihttp://hdl.handle.net/10754/596820en
dc.description.abstractThe Swi5-Sfr1 heterodimer protein stimulates the Rad51-promoted DNA strand exchange reaction, a crucial step in homologous recombination. To clarify how this accessory protein acts on the strand exchange reaction, we have analyzed how the structure of the primary reaction intermediate, the Rad51/single-stranded DNA (ssDNA) complex filament formed in the presence of ATP, is affected by Swi5-Sfr1. Using flow linear dichroism spectroscopy, we observe that the nucleobases of the ssDNA are more perpendicularly aligned to the filament axis in the presence of Swi5-Sfr1, whereas the bases are more randomly oriented in the absence of Swi5-Sfr1. When using a modified version of the natural protein where the N-terminal part of Sfr1 is deleted, which has no affinity for DNA but maintained ability to stimulate the strand exchange reaction, we still observe the improved perpendicular DNA base orientation. This indicates that Swi5-Sfr1 exerts its activating effect through interaction with the Rad51 filament mainly and not with the DNA. We propose that the role of a coplanar alignment of nucleobases induced by Swi5-Sfr1 in the presynaptic Rad51/ssDNA complex is to facilitate the critical matching with an invading double-stranded DNA, hence stimulating the strand exchange reaction.en
dc.description.sponsorshipKing Abdullah University of Science and Technology Grant [KUK-11-008-23 to L.F.]; the Agence Nationale de la Recherche Grant [ANR-2010-BLAN-1013 DynRec to A.R-C. and M.T.]; the European Research Council [ERC-2008-AdG 227700 to B.N.]; Ministry of Education, Culture, Sports, Science and Technology (MEXT) Japan [22125003 to H.I.]; and Japan Society for the Promotion of Science (JSPS) KAKENHI Grant [23770105 to N.K.]; The visit of M.T. to Japan was supported by JSPS Short-term Fellowship and that of N.K. to France by JSPS Institutional Program for Young Researcher Overseas Visits. Funding for open access charge: The Agence Nationale de la Recherche [ANR-2010-BLAN-1013].en
dc.publisherOxford University Press (OUP)en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subject.meshHomologous Recombinationen
dc.titleSwi5-Sfr1 protein stimulates Rad51-mediated DNA strand exchange reaction through organization of DNA bases in the presynaptic filament.en
dc.typeArticleen
dc.identifier.journalNucleic Acids Researchen
dc.identifier.pmcidPMC3936755en
dc.contributor.institutionDepartment of Chemical and Biological Engineering, Chalmers University of Technology, S-41296 Gothenburg, Sweden, Research Unit FRE3478, Centre National de la Recherche Scientifique & University of Nantes, F-44322 Nantes cedex 3, France, Graduate School of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan, Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, KEK, Tsukuba, 305-0801, Japan and Department of Life Science, Graduate School of Bioscience & Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.en
kaust.grant.numberKUK-11-008-23en

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