Microbial community changes along the active seepage site of one cold seep in the Red Sea.

Handle URI:
http://hdl.handle.net/10754/596802
Title:
Microbial community changes along the active seepage site of one cold seep in the Red Sea.
Authors:
Cao, Huiluo; Zhang, Weipeng; Wang, Yong; Qian, Pei-Yuan
Abstract:
The active seepage of the marine cold seeps could be a critical process for the exchange of energy between the submerged geosphere and the sea floor environment through organic-rich fluids, potentially even affecting surrounding microbial habitats. However, few studies have investigated the associated microbial community changes. In the present study, 16S rRNA genes were pyrosequenced to decipher changes in the microbial communities from the Thuwal seepage point in the Red Sea to nearby marine sediments in the brine pool, normal marine sediments and water, and benthic microbial mats. An unexpected number of reads from unclassified groups were detected in these habitats; however, the ecological functions of these groups remain unresolved. Furthermore, ammonia-oxidizing archaeal community structures were investigated using the ammonia monooxygenase subunit A (amoA) gene. Analysis of amoA showed that planktonic marine habitats, including seeps and marine water, hosted archaeal ammonia oxidizers that differed from those in microbial mats and marine sediments, suggesting modifications of the ammonia oxidizing archaeal (AOA) communities along the environmental gradient from active seepage sites to peripheral areas. Changes in the microbial community structure of AOA in different habitats (water vs. sediment) potentially correlated with changes in salinity and oxygen concentrations. Overall, the present results revealed for the first time unanticipated novel microbial groups and changes in the ammonia-oxidizing archaea in response to environmental gradients near the active seepages of a cold seep.
Citation:
Cao H, Zhang W, Wang Y, Qian P-Y (2015) Microbial community changes along the active seepage site of one cold seep in the Red Sea. Frontiers in Microbiology 6. Available: http://dx.doi.org/10.3389/fmicb.2015.00739.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
21-Jul-2015
DOI:
10.3389/fmicb.2015.00739
PubMed ID:
26284035
PubMed Central ID:
PMC4523032
Type:
Article
ISSN:
1664-302X
Sponsors:
This study was supported by the National Basic Research Program of China (973 Program, No: 2012CB417304), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB06010100 and XDB06010200), Exploration and Evaluation of Potential Resources from Deep-sea Microorganisms (DY125-15-R-02) from China Ocean Mineral Resources R&D Association (COMRRDA12SC02), and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology in Saudi Arabia to P-YQ. The authors are grateful to Alex Shek for his technical assistance. We thank Dr. Abdulaziz M Al-Suwailem and his team from the King Abdullah University of Science and Technology for organizing research cruise and assistance in collecting samples.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorCao, Huiluoen
dc.contributor.authorZhang, Weipengen
dc.contributor.authorWang, Yongen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2016-02-21T08:50:58Zen
dc.date.available2016-02-21T08:50:58Zen
dc.date.issued2015-07-21en
dc.identifier.citationCao H, Zhang W, Wang Y, Qian P-Y (2015) Microbial community changes along the active seepage site of one cold seep in the Red Sea. Frontiers in Microbiology 6. Available: http://dx.doi.org/10.3389/fmicb.2015.00739.en
dc.identifier.issn1664-302Xen
dc.identifier.pmid26284035en
dc.identifier.doi10.3389/fmicb.2015.00739en
dc.identifier.urihttp://hdl.handle.net/10754/596802en
dc.description.abstractThe active seepage of the marine cold seeps could be a critical process for the exchange of energy between the submerged geosphere and the sea floor environment through organic-rich fluids, potentially even affecting surrounding microbial habitats. However, few studies have investigated the associated microbial community changes. In the present study, 16S rRNA genes were pyrosequenced to decipher changes in the microbial communities from the Thuwal seepage point in the Red Sea to nearby marine sediments in the brine pool, normal marine sediments and water, and benthic microbial mats. An unexpected number of reads from unclassified groups were detected in these habitats; however, the ecological functions of these groups remain unresolved. Furthermore, ammonia-oxidizing archaeal community structures were investigated using the ammonia monooxygenase subunit A (amoA) gene. Analysis of amoA showed that planktonic marine habitats, including seeps and marine water, hosted archaeal ammonia oxidizers that differed from those in microbial mats and marine sediments, suggesting modifications of the ammonia oxidizing archaeal (AOA) communities along the environmental gradient from active seepage sites to peripheral areas. Changes in the microbial community structure of AOA in different habitats (water vs. sediment) potentially correlated with changes in salinity and oxygen concentrations. Overall, the present results revealed for the first time unanticipated novel microbial groups and changes in the ammonia-oxidizing archaea in response to environmental gradients near the active seepages of a cold seep.en
dc.description.sponsorshipThis study was supported by the National Basic Research Program of China (973 Program, No: 2012CB417304), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB06010100 and XDB06010200), Exploration and Evaluation of Potential Resources from Deep-sea Microorganisms (DY125-15-R-02) from China Ocean Mineral Resources R&D Association (COMRRDA12SC02), and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology in Saudi Arabia to P-YQ. The authors are grateful to Alex Shek for his technical assistance. We thank Dr. Abdulaziz M Al-Suwailem and his team from the King Abdullah University of Science and Technology for organizing research cruise and assistance in collecting samples.en
dc.publisherFrontiers Media SAen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject16S rRNA geneen
dc.subjectPyrosequencingen
dc.subjectRed Seaen
dc.subjectCold Seepen
dc.subjectAmmonia Oxidizing Archaeaen
dc.titleMicrobial community changes along the active seepage site of one cold seep in the Red Sea.en
dc.typeArticleen
dc.identifier.journalFrontiers in Microbiologyen
dc.identifier.pmcidPMC4523032en
dc.contributor.institutionDivision of Life Sciences, The Hong Kong University of Science and Technology Clear Water Bay, Hong Kong.en
dc.contributor.institutionDivision of Life Sciences, The Hong Kong University of Science and Technology Clear Water Bay, Hong Kong ; Sanya Institute of Deep Sea Science and Engineering, Chinese Academy of Sciences Sanya, China.en
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en

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