Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis.

Handle URI:
http://hdl.handle.net/10754/596782
Title:
Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis.
Authors:
Tian, Ren-Mao; Lee, On On; Wang, Yong; Cai, Lin; Bougouffa, Salim; Chiu, Jill Man Ying; Wu, Rudolf Shiu Sun; Qian, Pei-Yuan
Abstract:
Marine sponges play important roles in benthic environments and are sensitive to environmental stresses. Polybrominated diphenyl ethers (PBDEs) have been widely used as flame retardants since the 1970s and are cytotoxic and genotoxic to organisms. In the present study, we studied the short-period effect of PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) treatment on the community structure and functional gene composition of the bacterial community inhabiting the marine sponge Haliclona cymaeformis. Our results showed that the bacterial community shifted from an autotrophic bacteria-dominated community to a heterotrophic bacteria-dominated community in response to PBDE-47 in a time- and concentration-dependent manner. A potentially symbiotic sulfur-oxidizing bacterium (SOB) was dominant (>80% in abundance) in the untreated sponge. However, exposure to a high concentration (1 μg/L) of PBDE-47 caused a substantial decrease in the potential symbiont and an enrichment of heterotrophic bacteria like Clostridium. A metagenomic analysis showed a selective effect of the high concentration treatment on the functional gene composition of the enriched heterotrophic bacteria, revealing an enrichment for the functions responsible for DNA repair, multidrug efflux pumping, and bacterial chemotaxis and motility. This study demonstrated that PBDE-47 induced a shift in the composition of the community and functional genes in the sponge-associated bacterial community, revealing the selective effect of PBDE-47 treatment on the functions of the bacterial community in the microenvironment of the sponge.
Citation:
Tian R-M, Lee OO, Wang Y, Cai L, Bougouffa S, et al. (2015) Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis. Frontiers in Microbiology 5. Available: http://dx.doi.org/10.3389/fmicb.2014.00799.
Publisher:
Frontiers Media SA
Journal:
Frontiers in Microbiology
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
1-Jan-2014
DOI:
10.3389/fmicb.2014.00799
PubMed ID:
25642227
PubMed Central ID:
PMC4294214
Type:
Article
ISSN:
1664-302X
Sponsors:
This research is supported by the National Basic Research Program of China (973 Program, No: 2012CB417304), State Key Laboratory of Marine Pollution (SKLMP) Seed Collaborative Research fund (CITYU12SC01), the Area of Excellence (AoE) fund (AoE04/04-II) and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology granted to Pei-Yuan Qian.
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Publications Acknowledging KAUST Support

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DC FieldValue Language
dc.contributor.authorTian, Ren-Maoen
dc.contributor.authorLee, On Onen
dc.contributor.authorWang, Yongen
dc.contributor.authorCai, Linen
dc.contributor.authorBougouffa, Salimen
dc.contributor.authorChiu, Jill Man Yingen
dc.contributor.authorWu, Rudolf Shiu Sunen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2016-02-21T08:50:34Zen
dc.date.available2016-02-21T08:50:34Zen
dc.date.issued2014-01-01en
dc.identifier.citationTian R-M, Lee OO, Wang Y, Cai L, Bougouffa S, et al. (2015) Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis. Frontiers in Microbiology 5. Available: http://dx.doi.org/10.3389/fmicb.2014.00799.en
dc.identifier.issn1664-302Xen
dc.identifier.pmid25642227en
dc.identifier.doi10.3389/fmicb.2014.00799en
dc.identifier.urihttp://hdl.handle.net/10754/596782en
dc.description.abstractMarine sponges play important roles in benthic environments and are sensitive to environmental stresses. Polybrominated diphenyl ethers (PBDEs) have been widely used as flame retardants since the 1970s and are cytotoxic and genotoxic to organisms. In the present study, we studied the short-period effect of PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) treatment on the community structure and functional gene composition of the bacterial community inhabiting the marine sponge Haliclona cymaeformis. Our results showed that the bacterial community shifted from an autotrophic bacteria-dominated community to a heterotrophic bacteria-dominated community in response to PBDE-47 in a time- and concentration-dependent manner. A potentially symbiotic sulfur-oxidizing bacterium (SOB) was dominant (>80% in abundance) in the untreated sponge. However, exposure to a high concentration (1 μg/L) of PBDE-47 caused a substantial decrease in the potential symbiont and an enrichment of heterotrophic bacteria like Clostridium. A metagenomic analysis showed a selective effect of the high concentration treatment on the functional gene composition of the enriched heterotrophic bacteria, revealing an enrichment for the functions responsible for DNA repair, multidrug efflux pumping, and bacterial chemotaxis and motility. This study demonstrated that PBDE-47 induced a shift in the composition of the community and functional genes in the sponge-associated bacterial community, revealing the selective effect of PBDE-47 treatment on the functions of the bacterial community in the microenvironment of the sponge.en
dc.description.sponsorshipThis research is supported by the National Basic Research Program of China (973 Program, No: 2012CB417304), State Key Laboratory of Marine Pollution (SKLMP) Seed Collaborative Research fund (CITYU12SC01), the Area of Excellence (AoE) fund (AoE04/04-II) and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology granted to Pei-Yuan Qian.en
dc.publisherFrontiers Media SAen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSymbiontsen
dc.subjectMetagenomicsen
dc.subjectPbdesen
dc.subject16S Rrna Gene Pyrosequencingen
dc.subjectSponge-associated Bacterial Communityen
dc.titleEffect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis.en
dc.typeArticleen
dc.identifier.journalFrontiers in Microbiologyen
dc.identifier.pmcidPMC4294214en
dc.contributor.institutionDivison of Life Science, The Hong Kong University of Science and Technology Hong Kong, China.en
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist University Hong Kong, China.en
dc.contributor.institutionSchool of Biological Sciences, The University of Hong Kong Hong Kong, China ; State Key Laboratory of Marine Pollution, The City University of Hong Kong Hong Kong, China.en
dc.contributor.institutionDivison of Life Science, The Hong Kong University of Science and Technology Hong Kong, China ; State Key Laboratory of Marine Pollution, The City University of Hong Kong Hong Kong, China.en
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en

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