Convergent development of anodic bacterial communities in microbial fuel cells.

Handle URI:
http://hdl.handle.net/10754/596779
Title:
Convergent development of anodic bacterial communities in microbial fuel cells.
Authors:
Yates, Matthew D; Kiely, Patrick D; Call, Douglas F; Rismani-Yazdi, Hamid; Bibby, Kyle; Peccia, Jordan; Regan, John M; Logan, Bruce E
Abstract:
Microbial fuel cells (MFCs) are often inoculated from a single wastewater source. The extent that the inoculum affects community development or power production is unknown. The stable anodic microbial communities in MFCs were examined using three inocula: a wastewater treatment plant sample known to produce consistent power densities, a second wastewater treatment plant sample, and an anaerobic bog sediment. The bog-inoculated MFCs initially produced higher power densities than the wastewater-inoculated MFCs, but after 20 cycles all MFCs on average converged to similar voltages (470±20 mV) and maximum power densities (590±170 mW m(-2)). The power output from replicate bog-inoculated MFCs was not significantly different, but one wastewater-inoculated MFC (UAJA3 (UAJA, University Area Joint Authority Wastewater Treatment Plant)) produced substantially less power. Denaturing gradient gel electrophoresis profiling showed a stable exoelectrogenic biofilm community in all samples after 11 cycles. After 16 cycles the predominance of Geobacter spp. in anode communities was identified using 16S rRNA gene clone libraries (58±10%), fluorescent in-situ hybridization (FISH) (63±6%) and pyrosequencing (81±4%). While the clone library analysis for the underperforming UAJA3 had a significantly lower percentage of Geobacter spp. sequences (36%), suggesting that a predominance of this microbe was needed for convergent power densities, the lower percentage of this species was not verified by FISH or pyrosequencing analyses. These results show that the predominance of Geobacter spp. in acetate-fed systems was consistent with good MFC performance and independent of the inoculum source.
Citation:
Yates MD, Kiely PD, Call DF, Rismani-Yazdi H, Bibby K, et al. (2012) Convergent development of anodic bacterial communities in microbial fuel cells. ISME J 6: 2002–2013. Available: http://dx.doi.org/10.1038/ismej.2012.42.
Publisher:
Springer Nature
Journal:
The ISME Journal
KAUST Grant Number:
KUS-I1-003-13
Issue Date:
10-May-2012
DOI:
10.1038/ismej.2012.42
PubMed ID:
22572637
PubMed Central ID:
PMC3475369
Type:
Article
ISSN:
1751-7362; 1751-7370
Sponsors:
This research was supported by Award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST) and by Award DGE-0750756 from the National Science Foundation (NSF) Graduate Student Fellowship Program.
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorYates, Matthew Den
dc.contributor.authorKiely, Patrick Den
dc.contributor.authorCall, Douglas Fen
dc.contributor.authorRismani-Yazdi, Hamiden
dc.contributor.authorBibby, Kyleen
dc.contributor.authorPeccia, Jordanen
dc.contributor.authorRegan, John Men
dc.contributor.authorLogan, Bruce Een
dc.date.accessioned2016-02-21T08:50:30Zen
dc.date.available2016-02-21T08:50:30Zen
dc.date.issued2012-05-10en
dc.identifier.citationYates MD, Kiely PD, Call DF, Rismani-Yazdi H, Bibby K, et al. (2012) Convergent development of anodic bacterial communities in microbial fuel cells. ISME J 6: 2002–2013. Available: http://dx.doi.org/10.1038/ismej.2012.42.en
dc.identifier.issn1751-7362en
dc.identifier.issn1751-7370en
dc.identifier.pmid22572637en
dc.identifier.doi10.1038/ismej.2012.42en
dc.identifier.urihttp://hdl.handle.net/10754/596779en
dc.description.abstractMicrobial fuel cells (MFCs) are often inoculated from a single wastewater source. The extent that the inoculum affects community development or power production is unknown. The stable anodic microbial communities in MFCs were examined using three inocula: a wastewater treatment plant sample known to produce consistent power densities, a second wastewater treatment plant sample, and an anaerobic bog sediment. The bog-inoculated MFCs initially produced higher power densities than the wastewater-inoculated MFCs, but after 20 cycles all MFCs on average converged to similar voltages (470±20 mV) and maximum power densities (590±170 mW m(-2)). The power output from replicate bog-inoculated MFCs was not significantly different, but one wastewater-inoculated MFC (UAJA3 (UAJA, University Area Joint Authority Wastewater Treatment Plant)) produced substantially less power. Denaturing gradient gel electrophoresis profiling showed a stable exoelectrogenic biofilm community in all samples after 11 cycles. After 16 cycles the predominance of Geobacter spp. in anode communities was identified using 16S rRNA gene clone libraries (58±10%), fluorescent in-situ hybridization (FISH) (63±6%) and pyrosequencing (81±4%). While the clone library analysis for the underperforming UAJA3 had a significantly lower percentage of Geobacter spp. sequences (36%), suggesting that a predominance of this microbe was needed for convergent power densities, the lower percentage of this species was not verified by FISH or pyrosequencing analyses. These results show that the predominance of Geobacter spp. in acetate-fed systems was consistent with good MFC performance and independent of the inoculum source.en
dc.description.sponsorshipThis research was supported by Award KUS-I1-003-13 from the King Abdullah University of Science and Technology (KAUST) and by Award DGE-0750756 from the National Science Foundation (NSF) Graduate Student Fellowship Program.en
dc.publisherSpringer Natureen
dc.rightsThis work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subject.meshBacterial Typing Techniquesen
dc.subject.meshWater Microbiologyen
dc.titleConvergent development of anodic bacterial communities in microbial fuel cells.en
dc.typeArticleen
dc.identifier.journalThe ISME Journalen
dc.identifier.pmcidPMC3475369en
dc.contributor.institutionPennsylvania State University, State College, United Statesen
dc.contributor.institutionYale University, New Haven, United Statesen
kaust.grant.numberKUS-I1-003-13en

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