All-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.

Handle URI:
http://hdl.handle.net/10754/596765
Title:
All-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.
Authors:
So, Hongyun; Lee, Kunwoo; Murthy, Niren; Pisano, Albert P
Abstract:
This paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.
Citation:
So H, Lee K, Murthy N, Pisano AP (2014) All-in-One Nanowire-Decorated Multifunctional Membrane for Rapid Cell Lysis and Direct DNA Isolation. ACS Applied Materials & Interfaces 6: 20693–20699. Available: http://dx.doi.org/10.1021/am506153y.
Publisher:
American Chemical Society (ACS)
Journal:
ACS Applied Materials & Interfaces
Issue Date:
24-Nov-2014
DOI:
10.1021/am506153y
PubMed ID:
25420232
PubMed Central ID:
PMC4264858
Type:
Article
ISSN:
1944-8244; 1944-8252
Sponsors:
This work was partially supported by the grants from the KAUST Global Collaborative Research under the Academic Excellence Alliance program, the National Heart Lung and Blood Institute of the NIH as a Program of Excellence in the Nanotechnology award (HHSN268201000043C), RO1HL096796-02, and RO1AI088023-03. The authors are also grateful for support from Marvell Nanolab and Biomolecular Nanotechnology Center at UC Berkeley where all devices were fabricated. H.S. greatly acknowledges Prof. Young Ho Seo at Kangwon National University in Korea for his valuable discussion.
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Full metadata record

DC FieldValue Language
dc.contributor.authorSo, Hongyunen
dc.contributor.authorLee, Kunwooen
dc.contributor.authorMurthy, Nirenen
dc.contributor.authorPisano, Albert Pen
dc.date.accessioned2016-02-21T08:50:13Zen
dc.date.available2016-02-21T08:50:13Zen
dc.date.issued2014-11-24en
dc.identifier.citationSo H, Lee K, Murthy N, Pisano AP (2014) All-in-One Nanowire-Decorated Multifunctional Membrane for Rapid Cell Lysis and Direct DNA Isolation. ACS Applied Materials & Interfaces 6: 20693–20699. Available: http://dx.doi.org/10.1021/am506153y.en
dc.identifier.issn1944-8244en
dc.identifier.issn1944-8252en
dc.identifier.pmid25420232en
dc.identifier.doi10.1021/am506153yen
dc.identifier.urihttp://hdl.handle.net/10754/596765en
dc.description.abstractThis paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.en
dc.description.sponsorshipThis work was partially supported by the grants from the KAUST Global Collaborative Research under the Academic Excellence Alliance program, the National Heart Lung and Blood Institute of the NIH as a Program of Excellence in the Nanotechnology award (HHSN268201000043C), RO1HL096796-02, and RO1AI088023-03. The authors are also grateful for support from Marvell Nanolab and Biomolecular Nanotechnology Center at UC Berkeley where all devices were fabricated. H.S. greatly acknowledges Prof. Young Ho Seo at Kangwon National University in Korea for his valuable discussion.en
dc.publisherAmerican Chemical Society (ACS)en
dc.rightsThis is an open access article published under an ACS AuthorChoice , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.en
dc.subjectDNA purificationen
dc.subjectcell lysisen
dc.subjectNanowiresen
dc.subjectPoint-of-care Diagnosticsen
dc.subjectPorous Silicon Membraneen
dc.titleAll-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.en
dc.typeArticleen
dc.identifier.journalACS Applied Materials & Interfacesen
dc.identifier.pmcidPMC4264858en
dc.contributor.institutionDepartment of Mechanical Engineering, Berkeley Sensor & Actuator Center and ‡Department of Bioengineering, University of California , Berkeley, California 94720, United States.en
kaust.grant.programAcademic Excellence Alliance (AEA)en

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