A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA.

Handle URI:
http://hdl.handle.net/10754/596758
Title:
A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA.
Authors:
Cesana, Marcella; Cacchiarelli, Davide; Legnini, Ivano; Santini, Tiziana; Sthandier, Olga; Chinappi, Mauro; Tramontano, Anna; Bozzoni, Irene
Abstract:
Recently, a new regulatory circuitry has been identified in which RNAs can crosstalk with each other by competing for shared microRNAs. Such competing endogenous RNAs (ceRNAs) regulate the distribution of miRNA molecules on their targets and thereby impose an additional level of post-transcriptional regulation. Here we identify a muscle-specific long noncoding RNA, linc-MD1, which governs the time of muscle differentiation by acting as a ceRNA in mouse and human myoblasts. Downregulation or overexpression of linc-MD1 correlate with retardation or anticipation of the muscle differentiation program, respectively. We show that linc-MD1 "sponges" miR-133 and miR-133 [corrected] to regulate the expression of MAML1 and MEF2C, transcription factors that activate muscle-specific gene expression. Finally, we demonstrate that linc-MD1 exerts the same control over differentiation timing in human myoblasts, and that its levels are strongly reduced in Duchenne muscle cells. We conclude that the ceRNA network plays an important role in muscle differentiation.
Citation:
Cesana M, Cacchiarelli D, Legnini I, Santini T, Sthandier O, et al. (2011) A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA. Cell 147: 358–369. Available: http://dx.doi.org/10.1016/j.cell.2011.09.028.
Publisher:
Elsevier BV
Journal:
Cell
KAUST Grant Number:
KUK-I1-012-43
Issue Date:
1-Oct-2011
DOI:
10.1016/j.cell.2011.09.028
PubMed ID:
22000014
PubMed Central ID:
PMC3234495
Type:
Article
ISSN:
0092-8674
Sponsors:
We thank N. Proudfoot and K. Perkins for introducing M. C. to the 3C analysis; M. Mora and the Telethon Neuromuscular Biobank for providing material; J. Martone, V. Cazzella for useful discussion; and M. Marchioni for technical support. D.C. is a recipient of a Microsoft research PhD fellowship. This work was partially supported by grants from: Telethon (GGP07049), Parent Project Italia, EU project SIROCCO (LSHG-CT-2006-037900), KAUST KUK-I1-012-43, AIRC, IIT "SEED," FIRB, PRIN, and BEMM.
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Full metadata record

DC FieldValue Language
dc.contributor.authorCesana, Marcellaen
dc.contributor.authorCacchiarelli, Davideen
dc.contributor.authorLegnini, Ivanoen
dc.contributor.authorSantini, Tizianaen
dc.contributor.authorSthandier, Olgaen
dc.contributor.authorChinappi, Mauroen
dc.contributor.authorTramontano, Annaen
dc.contributor.authorBozzoni, Ireneen
dc.date.accessioned2016-02-21T08:50:02Zen
dc.date.available2016-02-21T08:50:02Zen
dc.date.issued2011-10-01en
dc.identifier.citationCesana M, Cacchiarelli D, Legnini I, Santini T, Sthandier O, et al. (2011) A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA. Cell 147: 358–369. Available: http://dx.doi.org/10.1016/j.cell.2011.09.028.en
dc.identifier.issn0092-8674en
dc.identifier.pmid22000014en
dc.identifier.doi10.1016/j.cell.2011.09.028en
dc.identifier.urihttp://hdl.handle.net/10754/596758en
dc.description.abstractRecently, a new regulatory circuitry has been identified in which RNAs can crosstalk with each other by competing for shared microRNAs. Such competing endogenous RNAs (ceRNAs) regulate the distribution of miRNA molecules on their targets and thereby impose an additional level of post-transcriptional regulation. Here we identify a muscle-specific long noncoding RNA, linc-MD1, which governs the time of muscle differentiation by acting as a ceRNA in mouse and human myoblasts. Downregulation or overexpression of linc-MD1 correlate with retardation or anticipation of the muscle differentiation program, respectively. We show that linc-MD1 "sponges" miR-133 and miR-133 [corrected] to regulate the expression of MAML1 and MEF2C, transcription factors that activate muscle-specific gene expression. Finally, we demonstrate that linc-MD1 exerts the same control over differentiation timing in human myoblasts, and that its levels are strongly reduced in Duchenne muscle cells. We conclude that the ceRNA network plays an important role in muscle differentiation.en
dc.description.sponsorshipWe thank N. Proudfoot and K. Perkins for introducing M. C. to the 3C analysis; M. Mora and the Telethon Neuromuscular Biobank for providing material; J. Martone, V. Cazzella for useful discussion; and M. Marchioni for technical support. D.C. is a recipient of a Microsoft research PhD fellowship. This work was partially supported by grants from: Telethon (GGP07049), Parent Project Italia, EU project SIROCCO (LSHG-CT-2006-037900), KAUST KUK-I1-012-43, AIRC, IIT "SEED," FIRB, PRIN, and BEMM.en
dc.publisherElsevier BVen
dc.rightsThis document may be redistributed and reused, subject to http://www.elsevier.com/wps/find/authorsview.authors/supplementalterms1.0.en
dc.subject.meshGene Expression Regulation, Developmentalen
dc.subject.meshMuscle Developmenten
dc.titleA long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA.en
dc.typeArticleen
dc.identifier.journalCellen
dc.identifier.pmcidPMC3234495en
dc.contributor.institutionDepartment of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, Italy.en
kaust.grant.numberKUK-I1-012-43en

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