Identification of a Stelar-Localized Transport Protein That Facilitates Root-to-Shoot Transfer of Chloride in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/596021
Title:
Identification of a Stelar-Localized Transport Protein That Facilitates Root-to-Shoot Transfer of Chloride in Arabidopsis
Authors:
Li, Bo; Byrt, Caitlin; Qiu, Jiaen; Baumann, Ute; Hrmova, Maria; Evrard, Aurelie; Johnson, Alexander A T; Birnbaum, Kenneth D.; Mayo, Gwenda M.; Jha, Deepa; Henderson, Sam W.; Tester, Mark A. ( 0000-0002-5085-8801 ) ; Gilliham, Mathew; Roy, Stuart J.
Abstract:
Under saline conditions, higher plants restrict the accumulation of chloride ions (Cl–) in the shoot by regulating their transfer from the root symplast into the xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen of salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified a candidate gene involved in Cl– xylem loading from the Nitrate transporter 1/Peptide Transporter family (NPF2.4). This gene was highly expressed in the root stele compared to the cortex, and its expression decreased after exposure to NaCl or abscisic acid. NPF2.4 fused to fluorescent proteins, expressed either transiently or stably, was targeted to the plasma membrane. Electrophysiological analysis of NPF2.4 in Xenopus laevis oocytes suggested that NPF2.4 catalyzed passive Cl– efflux out of cells and was much less permeable to NO3−. Shoot Cl– accumulation was decreased following NPF2.4 artificial microRNA knockdown, whereas it was increased by overexpression of NPF2.4. Taken together, these results suggest that NPF2.4 is involved in long-distance transport of Cl– in plants, playing a role in the loading and the regulation of Cl– loading into the xylem of Arabidopsis roots during salinity stress.
KAUST Department:
Center for Desert Agriculture
Citation:
Li, B., Byrt, C.S., Qiu, J., Baumann, U., Hrmova, M., Evrard, A., Johnson, A.A., Birnbaum, K.D., Mayo, G.M., Jha, D. and Henderson, S.W., 2015. Identification of a stelar-localised transport protein that facilitates root-to-shoot transfer of chloride in Arabidopsis. Plant physiology, pp.pp-01163.
Publisher:
American Society of Plant Biologists
Journal:
Plant Physiology
Issue Date:
11-Dec-2015
DOI:
10.​1104/​pp.​15.​01163
Type:
Article
ISSN:
0032-0889
Additional Links:
http://www.plantphysiol.org/content/170/2/1014
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Boen
dc.contributor.authorByrt, Caitlinen
dc.contributor.authorQiu, Jiaenen
dc.contributor.authorBaumann, Uteen
dc.contributor.authorHrmova, Mariaen
dc.contributor.authorEvrard, Aurelieen
dc.contributor.authorJohnson, Alexander A Ten
dc.contributor.authorBirnbaum, Kenneth D.en
dc.contributor.authorMayo, Gwenda M.en
dc.contributor.authorJha, Deepaen
dc.contributor.authorHenderson, Sam W.en
dc.contributor.authorTester, Mark A.en
dc.contributor.authorGilliham, Mathewen
dc.contributor.authorRoy, Stuart J.en
dc.date.accessioned2016-02-10T13:21:23Zen
dc.date.available2016-02-10T13:21:23Zen
dc.date.issued2015-12-11en
dc.identifier.citationLi, B., Byrt, C.S., Qiu, J., Baumann, U., Hrmova, M., Evrard, A., Johnson, A.A., Birnbaum, K.D., Mayo, G.M., Jha, D. and Henderson, S.W., 2015. Identification of a stelar-localised transport protein that facilitates root-to-shoot transfer of chloride in Arabidopsis. Plant physiology, pp.pp-01163.en
dc.identifier.issn0032-0889en
dc.identifier.doi10.​1104/​pp.​15.​01163en
dc.identifier.urihttp://hdl.handle.net/10754/596021en
dc.description.abstractUnder saline conditions, higher plants restrict the accumulation of chloride ions (Cl–) in the shoot by regulating their transfer from the root symplast into the xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen of salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified a candidate gene involved in Cl– xylem loading from the Nitrate transporter 1/Peptide Transporter family (NPF2.4). This gene was highly expressed in the root stele compared to the cortex, and its expression decreased after exposure to NaCl or abscisic acid. NPF2.4 fused to fluorescent proteins, expressed either transiently or stably, was targeted to the plasma membrane. Electrophysiological analysis of NPF2.4 in Xenopus laevis oocytes suggested that NPF2.4 catalyzed passive Cl– efflux out of cells and was much less permeable to NO3−. Shoot Cl– accumulation was decreased following NPF2.4 artificial microRNA knockdown, whereas it was increased by overexpression of NPF2.4. Taken together, these results suggest that NPF2.4 is involved in long-distance transport of Cl– in plants, playing a role in the loading and the regulation of Cl– loading into the xylem of Arabidopsis roots during salinity stress.en
dc.language.isoenen
dc.publisherAmerican Society of Plant Biologistsen
dc.relation.urlhttp://www.plantphysiol.org/content/170/2/1014en
dc.rightsArchived with thanks to Plant Physiologyen
dc.titleIdentification of a Stelar-Localized Transport Protein That Facilitates Root-to-Shoot Transfer of Chloride in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalPlant Physiologyen
dc.eprint.versionPost-printen
dc.contributor.institutionAustralian Centre for Plant Functional Genomicsen
dc.contributor.institutionARC Centre of Excellence in Plant Energy Biologyen
dc.contributor.institutionUniversity of Adelaide, SA 5064, Australiaen
dc.contributor.institutionSchool of BioSciences, University of Melbourne, Parkville, Vic 3010, Australiaen
dc.contributor.institutionCentre for Genomics and Systems Biology, New York University, New York 10003en
dc.contributor.institutionSchool of Agriculture, Food, and Wineen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorShamma, Jeff S.en
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