Method of detaching adherent cells for flow cytometry

Handle URI:
http://hdl.handle.net/10754/595597
Title:
Method of detaching adherent cells for flow cytometry
Authors:
Kaur, Mandeep; Esau, Luke E.
Assignee:
King Abdullah University of Science and Technology
Abstract:
In one aspect, a method for detaching adherent cells can include adding a cell lifting solution to the media including a sample of adherent cells and incubating the sample of adherent cells with the cell lifting solution. No scraping or pipetting is needed to facilitate cell detachment. The method do not require inactivation of cell lifting solution and no washing of detaching cells is required to remove cell lifting solution. Detached cells can be stained with dye in the presence of cell lifting solution and are further analyzed using flow cytometer. The method has been tested using 6 different cell lines, 4 different assays, two different plate formats (96 and 384 well plates) and two different flow cytometry instruments. The method is simple to perform, less time consuming, with no cell loss and makes high throughput flow cytometry on adherent cells a reality.
KAUST Department:
Computational Bioscience Research Center (CBRC); Technology Transfer
Issue Date:
24-Dec-2015
Submitted date:
2015-07-29
Type:
Patent
Application Number:
US 20150368620 A1
Patent Status:
Published Application
Additional Links:
http://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.html&r=1&f=G&l=50&s1=%2220150368620%22.PGNR.&OS=DN/20150368620&RS=DN/20150368620; http://assignment.uspto.gov/#/search?adv=publNum:20150368620; http://www.google.com/patents/US20150333282; http://worldwide.espacenet.com/publicationDetails/biblio?CC=US&NR=2015333282A1&KC=A1&FT=D
Appears in Collections:
Patents; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorKaur, Mandeepen
dc.contributor.authorEsau, Luke E.en
dc.date.accessioned2016-02-04T13:33:33Zen
dc.date.available2016-02-04T13:33:33Zen
dc.date.issued2015-12-24en
dc.date.submitted2015-07-29en
dc.identifier.urihttp://hdl.handle.net/10754/595597en
dc.description.abstractIn one aspect, a method for detaching adherent cells can include adding a cell lifting solution to the media including a sample of adherent cells and incubating the sample of adherent cells with the cell lifting solution. No scraping or pipetting is needed to facilitate cell detachment. The method do not require inactivation of cell lifting solution and no washing of detaching cells is required to remove cell lifting solution. Detached cells can be stained with dye in the presence of cell lifting solution and are further analyzed using flow cytometer. The method has been tested using 6 different cell lines, 4 different assays, two different plate formats (96 and 384 well plates) and two different flow cytometry instruments. The method is simple to perform, less time consuming, with no cell loss and makes high throughput flow cytometry on adherent cells a reality.en
dc.relation.urlhttp://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.html&r=1&f=G&l=50&s1=%2220150368620%22.PGNR.&OS=DN/20150368620&RS=DN/20150368620en
dc.relation.urlhttp://assignment.uspto.gov/#/search?adv=publNum:20150368620en
dc.relation.urlhttp://www.google.com/patents/US20150333282en
dc.relation.urlhttp://worldwide.espacenet.com/publicationDetails/biblio?CC=US&NR=2015333282A1&KC=A1&FT=Den
dc.titleMethod of detaching adherent cells for flow cytometryen
dc.typePatenten
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentTechnology Transferen
dc.description.statusPublished Applicationen
dc.contributor.assigneeKing Abdullah University of Science and Technologyen
dc.description.countryUnited Statesen
dc.identifier.applicationnumberUS 20150368620 A1en
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