Evaluation of multiple theranostic properties of polydopamine coated Fe0.65Co0.35@(Fe0.65Co0.35)3O4 nanocubes for cancer nanomedicinal application

Handle URI:
http://hdl.handle.net/10754/595129
Title:
Evaluation of multiple theranostic properties of polydopamine coated Fe0.65Co0.35@(Fe0.65Co0.35)3O4 nanocubes for cancer nanomedicinal application
Authors:
Julfakyan, Khachatur ( 0000-0003-2482-0911 ) ; Fatieiev, Yevhen ( 0000-0002-2844-8427 ) ; Alsaiari, Shahad K.; Deng, L.; Ezzeddine, Alaa; Abu Samra, Dina Bashir Kamil ( 0000-0002-0974-5362 ) ; Khashab, Niveen M. ( 0000-0003-2728-0666 )
Abstract:
The objective of our work was the fabrication of single theranostic agent for cancer nanomedicine, which should be easily producible, cheap, biocompatible, spatially and temporally controllable and should have broader range of diagnostic and therapeutic modalities compared with previous works. Here we present the cost efficient and easy to scale-up, modified procedure of synthesis, controlled thin oxide shell formation and polydopamine coating with basic polymerization of dopamine hydrochloride in aqueous solution. Full characterization (HR-TEM, SEM, XRD, HR-TEM-EDAX, HR-TEM-SAED, SEM-EDAX, FTIR, UV-Vis-NIR, TGA, VSM) of novel system comprising of sub-micrometer sized Fe0.65Co0.35@(Fe0.65Co0.35)3O4@PDA highly magnetic (with 226 emu/g value for the metal core Fe0.65Co0.35@(Fe0.65Co0.35)3O4) nanoparticles was done. In vitro MTT cell viability assay on HeLa cells showed no toxicity up to 100 µg/ml of nanoparticles concentration. They are dual mode diagnostic agents with MRI (r2=186.44 mM-1s-1) and X-Ray CT contrasting properties. The HeLa cells was used in in vitro assays. Doxorubicin delivery to the nuclei (laser fluorescence confocal microscopy) and 55% of death (MTT assay) at the concentration of 10 µg/ml was registered. The release of doxorubicin was demonstrated pH sensitive and attenuated in time behavior (temporal control). Specific absorption rate for 470 kHz alternating magnetic field hyperthermia was 180 W/g. Flow cytometry with PI staining demonstrated 60% cell death with 15 min of 808 nm NIR laser exposure at 0.3 kW of laser power and 30 µg/ml of nanoparticles concentration. High magnetic saturation is prerequisite for potential magnetic targeting (special control), the size is within the range of EPR mediated accumulation. Summarizing, above mentioned nanocomposite has 5 theranostic modalities, which is more than previous works and may be considered as good candidate for further in vivo studies as “all-in-one” agent.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Chemical Science Program; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Advanced Membranes and Porous Materials Research Center; Smart Hybrid Materials (SHMs) lab; CMS Lab.
Conference/Event name:
International Conference on Advances in Functional Materials (AFM 2015)
Issue Date:
29-Jun-2015
Type:
Presentation
Additional Links:
http://afm2015.functionalmaterials.org/wp-content/uploads/2015/07/S5-Abs.pdf
Appears in Collections:
Bioscience Program; Advanced Membranes and Porous Materials Research Center; Physical Sciences and Engineering (PSE) Division; Controlled Release and Delivery Laboratory; Chemical Science Program; Presentations; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorJulfakyan, Khachaturen
dc.contributor.authorFatieiev, Yevhenen
dc.contributor.authorAlsaiari, Shahad K.en
dc.contributor.authorDeng, L.en
dc.contributor.authorEzzeddine, Alaaen
dc.contributor.authorAbu Samra, Dina Bashir Kamilen
dc.contributor.authorKhashab, Niveen M.en
dc.date.accessioned2016-01-28T07:14:08Zen
dc.date.available2016-01-28T07:14:08Zen
dc.date.issued2015-06-29en
dc.identifier.urihttp://hdl.handle.net/10754/595129en
dc.description.abstractThe objective of our work was the fabrication of single theranostic agent for cancer nanomedicine, which should be easily producible, cheap, biocompatible, spatially and temporally controllable and should have broader range of diagnostic and therapeutic modalities compared with previous works. Here we present the cost efficient and easy to scale-up, modified procedure of synthesis, controlled thin oxide shell formation and polydopamine coating with basic polymerization of dopamine hydrochloride in aqueous solution. Full characterization (HR-TEM, SEM, XRD, HR-TEM-EDAX, HR-TEM-SAED, SEM-EDAX, FTIR, UV-Vis-NIR, TGA, VSM) of novel system comprising of sub-micrometer sized Fe0.65Co0.35@(Fe0.65Co0.35)3O4@PDA highly magnetic (with 226 emu/g value for the metal core Fe0.65Co0.35@(Fe0.65Co0.35)3O4) nanoparticles was done. In vitro MTT cell viability assay on HeLa cells showed no toxicity up to 100 µg/ml of nanoparticles concentration. They are dual mode diagnostic agents with MRI (r2=186.44 mM-1s-1) and X-Ray CT contrasting properties. The HeLa cells was used in in vitro assays. Doxorubicin delivery to the nuclei (laser fluorescence confocal microscopy) and 55% of death (MTT assay) at the concentration of 10 µg/ml was registered. The release of doxorubicin was demonstrated pH sensitive and attenuated in time behavior (temporal control). Specific absorption rate for 470 kHz alternating magnetic field hyperthermia was 180 W/g. Flow cytometry with PI staining demonstrated 60% cell death with 15 min of 808 nm NIR laser exposure at 0.3 kW of laser power and 30 µg/ml of nanoparticles concentration. High magnetic saturation is prerequisite for potential magnetic targeting (special control), the size is within the range of EPR mediated accumulation. Summarizing, above mentioned nanocomposite has 5 theranostic modalities, which is more than previous works and may be considered as good candidate for further in vivo studies as “all-in-one” agent.en
dc.relation.urlhttp://afm2015.functionalmaterials.org/wp-content/uploads/2015/07/S5-Abs.pdfen
dc.subjecttheranostic nanoparticlesen
dc.subjectdrug deliveryen
dc.subjectmagnetic hyperthermiaen
dc.subjectphotothermal therapyen
dc.subjectcanceren
dc.titleEvaluation of multiple theranostic properties of polydopamine coated Fe0.65Co0.35@(Fe0.65Co0.35)3O4 nanocubes for cancer nanomedicinal applicationen
dc.typePresentationen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentChemical Science Programen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Centeren
dc.contributor.departmentSmart Hybrid Materials (SHMs) laben
dc.contributor.departmentCMS Lab.en
dc.conference.date29 June – 3 July 2015en
dc.conference.nameInternational Conference on Advances in Functional Materials (AFM 2015)en
dc.conference.locationStony Brook University, NY State, USAen
kaust.authorJulfakyan, Khachaturen
kaust.authorFatieiev, Yevhenen
kaust.authorAlsaiari, Shahad K.en
kaust.authorDeng, L.en
kaust.authorEzzeddine, Alaaen
kaust.authorAbu Samra, Dina Bashir Kamilen
kaust.authorKhashab, Niveen M.en
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