Nutrient Limitation in Surface Waters of the Oligotrophic Eastern Mediterranean Sea: an Enrichment Microcosm Experiment

Handle URI:
http://hdl.handle.net/10754/594983
Title:
Nutrient Limitation in Surface Waters of the Oligotrophic Eastern Mediterranean Sea: an Enrichment Microcosm Experiment
Authors:
Tsiola, A.; Pitta, P.; Fodelianakis, Stylianos ( 0000-0003-2186-6009 ) ; Pete, R.; Magiopoulos, I.; Mara, P.; Psarra, S.; Tanaka, T.; Mostajir, B.
Abstract:
The growth rates of planktonic microbes in the pelagic zone of the Eastern Mediterranean Sea are nutrient limited, but the type of limitation is still uncertain. During this study, we investigated the occurrence of N and P limitation among different groups of the prokaryotic and eukaryotic (pico-, nano-, and micro-) plankton using a microcosm experiment during stratified water column conditions in the Cretan Sea (Eastern Mediterranean). Microcosms were enriched with N and P (either solely or simultaneously), and the PO4 turnover time, prokaryotic heterotrophic activity, primary production, and the abundance of the different microbial components were measured. Flow cytometric and molecular fingerprint analyses showed that different heterotrophic prokaryotic groups were limited by different nutrients; total heterotrophic prokaryotic growth was limited by P, but only when both N and P were added, changes in community structure and cell size were detected. Phytoplankton were N and P co-limited, with autotrophic pico-eukaryotes being the exception as they increased even when only P was added after a 2-day time lag. The populations of Synechococcus and Prochlorococcus were highly competitive with each other; Prochlorococcus abundance increased during the first 2 days of P addition but kept increasing only when both N and P were added, whereas Synechococcus exhibited higher pigment content and increased in abundance 3 days after simultaneous N and P additions. Dinoflagellates also showed opportunistic behavior at simultaneous N and P additions, in contrast to diatoms and coccolithophores, which diminished in all incubations. High DNA content viruses, selective grazing, and the exhaustion of N sources probably controlled the populations of diatoms and coccolithophores.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Nutrient Limitation in Surface Waters of the Oligotrophic Eastern Mediterranean Sea: an Enrichment Microcosm Experiment 2015 Microbial Ecology
Publisher:
Springer Science + Business Media
Journal:
Microbial Ecology
Issue Date:
1-Dec-2015
DOI:
10.1007/s00248-015-0713-5
Type:
Article
ISSN:
0095-3628; 1432-184X
Additional Links:
http://link.springer.com/10.1007/s00248-015-0713-5
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorTsiola, A.en
dc.contributor.authorPitta, P.en
dc.contributor.authorFodelianakis, Stylianosen
dc.contributor.authorPete, R.en
dc.contributor.authorMagiopoulos, I.en
dc.contributor.authorMara, P.en
dc.contributor.authorPsarra, S.en
dc.contributor.authorTanaka, T.en
dc.contributor.authorMostajir, B.en
dc.date.accessioned2016-01-27T13:15:08Zen
dc.date.available2016-01-27T13:15:08Zen
dc.date.issued2015-12-01en
dc.identifier.citationNutrient Limitation in Surface Waters of the Oligotrophic Eastern Mediterranean Sea: an Enrichment Microcosm Experiment 2015 Microbial Ecologyen
dc.identifier.issn0095-3628en
dc.identifier.issn1432-184Xen
dc.identifier.doi10.1007/s00248-015-0713-5en
dc.identifier.urihttp://hdl.handle.net/10754/594983en
dc.description.abstractThe growth rates of planktonic microbes in the pelagic zone of the Eastern Mediterranean Sea are nutrient limited, but the type of limitation is still uncertain. During this study, we investigated the occurrence of N and P limitation among different groups of the prokaryotic and eukaryotic (pico-, nano-, and micro-) plankton using a microcosm experiment during stratified water column conditions in the Cretan Sea (Eastern Mediterranean). Microcosms were enriched with N and P (either solely or simultaneously), and the PO4 turnover time, prokaryotic heterotrophic activity, primary production, and the abundance of the different microbial components were measured. Flow cytometric and molecular fingerprint analyses showed that different heterotrophic prokaryotic groups were limited by different nutrients; total heterotrophic prokaryotic growth was limited by P, but only when both N and P were added, changes in community structure and cell size were detected. Phytoplankton were N and P co-limited, with autotrophic pico-eukaryotes being the exception as they increased even when only P was added after a 2-day time lag. The populations of Synechococcus and Prochlorococcus were highly competitive with each other; Prochlorococcus abundance increased during the first 2 days of P addition but kept increasing only when both N and P were added, whereas Synechococcus exhibited higher pigment content and increased in abundance 3 days after simultaneous N and P additions. Dinoflagellates also showed opportunistic behavior at simultaneous N and P additions, in contrast to diatoms and coccolithophores, which diminished in all incubations. High DNA content viruses, selective grazing, and the exhaustion of N sources probably controlled the populations of diatoms and coccolithophores.en
dc.language.isoenen
dc.publisherSpringer Science + Business Mediaen
dc.relation.urlhttp://link.springer.com/10.1007/s00248-015-0713-5en
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/s00248-015-0713-5.en
dc.subjectSynechococcusen
dc.subjectProchlorococcusen
dc.subjectHeterotrophic prokaryotesen
dc.subjectVirusesen
dc.subjectVirusesen
dc.subjectEukaryotic phytoplanktonen
dc.titleNutrient Limitation in Surface Waters of the Oligotrophic Eastern Mediterranean Sea: an Enrichment Microcosm Experimenten
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMicrobial Ecologyen
dc.eprint.versionPost-printen
dc.contributor.institutionInstitute of Oceanography, Hellenic Centre for Marine Research (HCMR), Ex American Base Gournes, P.O. Box 2214, 71003, Heraklion, Crete, Greeceen
dc.contributor.institutionBiology Department, Marine Ecology Laboratory, University of Crete, Heraklion, Crete, Greeceen
dc.contributor.institutionLaboratoire d’Ecologie des Systèmes Marins Côtiers (ECOSYM), CNRS–Université Montpellier 2 & 1–Ifremer–IRD, Montpellier, Franceen
dc.contributor.institutionChemistry Department, Environmental Chemical Processes Laboratory, University of Crete, Heraklion, Crete, Greeceen
dc.contributor.institutionINSU-CNRS, Laboratoire d’Océanographie de Villefranche, Villefranche sur Mer cedex, Franceen
dc.contributor.institutionUniversité Pierre et Marie Curie-Paris 6, Observatoire Océanologie de Villefranche, Villefranche sur Mer cedex, Franceen
dc.contributor.institutionCentre d’Ecologie Marine Expérimentale MEDIMEER, Mediterranean Center for Marine Ecosystem Experimental Research, CNRS–Université Montpellier 2, Montpellier, Sète, Franceen
dc.contributor.institutionMarine Biodiversity, Exploitation and Conservation (MARBEC), UMR 9190, CNRS–Université de Montpellier–IFREMER–IRD, Montpellier, Franceen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorFodelianakis, Stylianosen
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