Comparative analysis and culturing of the microbial community of Aiptasia pallida, A Sea Anemone Model for Coral Biology

Handle URI:
http://hdl.handle.net/10754/594745
Title:
Comparative analysis and culturing of the microbial community of Aiptasia pallida, A Sea Anemone Model for Coral Biology
Authors:
Binsarhan, Mohammad ( 0000-0003-4694-3699 )
Abstract:
Recent works has highlighted the contribution of microbes to animal function. In this regard, the microbial community associated with corals has become a growing field of research in order to understand how microbes contribute to the host organisms’ response to environmental changes. It has been shown that microbes associated with corals have important functions in the coral holobiont such as immunity and nutrient assimilation. However, corals are notoriously difficult to work with. To this end, the sea anemone Aiptasia is becoming a model organism for coral symbiosis. Given the importance of host-­microbiome interactions, the topic of this thesis is to assess microbial structure of Aiptasia, culture prominent bacterial members, and compare bacterial community structure to corals. Different molecular methods have been applied using 16S rRNA bacterial gene fragments to characterize the microbial composition of Aiptasia. 16S rRNA gene sequence derived from cultured bacteria was compared to 16S rRNA gene sequences retrieved from native Red Sea Aiptasia. Inter-­individual as well as methodological differences were found to account for variance in microbiome composition. However, all approaches showed a highly abundant microbial taxon belonging to the genus Alteromonas in all samples. The Alteromonas species was successfully isolated for further research targeting microbiome selection mechanisms in Aiptasia. Future investigations by using different molecular tools will help to define the functions and relationship between the Aiptasia and its complex microbiome.
Advisors:
Voolstra, Christian R. ( 0000-0003-4555-3795 )
Committee Member:
Berumen, Michael L. ( 0000-0003-2463-2742 ) ; Aranda, Manuel ( 0000-0001-6673-016X )
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program
Program:
Marine Science
Issue Date:
Jan-2016
Type:
Thesis
Appears in Collections:
Marine Science Program; Theses; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorVoolstra, Christian R.en
dc.contributor.authorBinsarhan, Mohammaden
dc.date.accessioned2016-01-24T12:26:44Zen
dc.date.available2016-01-24T12:26:44Zen
dc.date.issued2016-01en
dc.identifier.urihttp://hdl.handle.net/10754/594745en
dc.description.abstractRecent works has highlighted the contribution of microbes to animal function. In this regard, the microbial community associated with corals has become a growing field of research in order to understand how microbes contribute to the host organisms’ response to environmental changes. It has been shown that microbes associated with corals have important functions in the coral holobiont such as immunity and nutrient assimilation. However, corals are notoriously difficult to work with. To this end, the sea anemone Aiptasia is becoming a model organism for coral symbiosis. Given the importance of host-­microbiome interactions, the topic of this thesis is to assess microbial structure of Aiptasia, culture prominent bacterial members, and compare bacterial community structure to corals. Different molecular methods have been applied using 16S rRNA bacterial gene fragments to characterize the microbial composition of Aiptasia. 16S rRNA gene sequence derived from cultured bacteria was compared to 16S rRNA gene sequences retrieved from native Red Sea Aiptasia. Inter-­individual as well as methodological differences were found to account for variance in microbiome composition. However, all approaches showed a highly abundant microbial taxon belonging to the genus Alteromonas in all samples. The Alteromonas species was successfully isolated for further research targeting microbiome selection mechanisms in Aiptasia. Future investigations by using different molecular tools will help to define the functions and relationship between the Aiptasia and its complex microbiome.en
dc.language.isoenen
dc.subjectAiptasiaen
dc.subjectMicrobiomeen
dc.subjectAlteromonasen
dc.subjectCoralen
dc.subjectNative Bacteriaen
dc.subjectCulturable Bacteriaen
dc.titleComparative analysis and culturing of the microbial community of Aiptasia pallida, A Sea Anemone Model for Coral Biologyen
dc.typeThesisen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberBerumen, Michael L.en
dc.contributor.committeememberAranda, Manuelen
thesis.degree.disciplineMarine Scienceen
thesis.degree.nameMaster of Scienceen
dc.person.id134675en
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