A primer of statistical methods for correlating parameters and properties of electrospun poly( l -lactide) scaffolds for tissue engineering-PART 2: Regression

Handle URI:
http://hdl.handle.net/10754/594264
Title:
A primer of statistical methods for correlating parameters and properties of electrospun poly( l -lactide) scaffolds for tissue engineering-PART 2: Regression
Authors:
Seyedmahmoud, Rasoul; Mozetic, Pamela; Rainer, Alberto; Giannitelli, Sara Maria; Basoli, Francesco; Trombetta, Marcella; Traversa, Enrico; Licoccia, Silvia; Rinaldi, Antonio
Abstract:
This two-articles series presents an in-depth discussion of electrospun poly-l-lactide scaffolds for tissue engineering by means of statistical methodologies that can be used, in general, to gain a quantitative and systematic insight about effects and interactions between a handful of key scaffold properties (Ys) and a set of process parameters (Xs) in electrospinning. While Part-1 dealt with the DOE methods to unveil the interactions between Xs in determining the morphomechanical properties (ref. Y1-4), this Part-2 article continues and refocuses the discussion on the interdependence of scaffold properties investigated by standard regression methods. The discussion first explores the connection between mechanical properties (Y4) and morphological descriptors of the scaffolds (Y1-3) in 32 types of scaffolds, finding that the mean fiber diameter (Y1) plays a predominant role which is nonetheless and crucially modulated by the molecular weight (MW) of PLLA. The second part examines the biological performance (Y5) (i.e. the cell proliferation of seeded bone marrow-derived mesenchymal stromal cells) on a random subset of eight scaffolds vs. the mechanomorphological properties (Y1-4). In this case, the featured regression analysis on such an incomplete set was not conclusive, though, indirectly suggesting in quantitative terms that cell proliferation could not fully be explained as a function of considered mechanomorphological properties (Y1-4), but in the early stage seeding, and that a randomization effects occurs over time such that the differences in initial cell proliferation performance (at day 1) is smeared over time. The findings may be the cornerstone of a novel route to accrue sufficient understanding and establish design rules for scaffold biofunctional vs. architecture, mechanical properties, and process parameters.
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Citation:
Seyedmahmoud R, Mozetic P, Rainer A, Giannitelli SM, Basoli F, et al. (2014) A primer of statistical methods for correlating parameters and properties of electrospun poly( l -lactide) scaffolds for tissue engineering-PART 2: Regression . J Biomed Mater Res 103: 103–114. Available: http://dx.doi.org/10.1002/jbm.a.35183.
Publisher:
Wiley-Blackwell
Journal:
Journal of Biomedical Materials Research Part A
Issue Date:
7-Apr-2014
DOI:
10.1002/jbm.a.35183
PubMed ID:
24668730
Type:
Article
ISSN:
1549-3296
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSeyedmahmoud, Rasoulen
dc.contributor.authorMozetic, Pamelaen
dc.contributor.authorRainer, Albertoen
dc.contributor.authorGiannitelli, Sara Mariaen
dc.contributor.authorBasoli, Francescoen
dc.contributor.authorTrombetta, Marcellaen
dc.contributor.authorTraversa, Enricoen
dc.contributor.authorLicoccia, Silviaen
dc.contributor.authorRinaldi, Antonioen
dc.date.accessioned2016-01-19T14:44:41Zen
dc.date.available2016-01-19T14:44:41Zen
dc.date.issued2014-04-07en
dc.identifier.citationSeyedmahmoud R, Mozetic P, Rainer A, Giannitelli SM, Basoli F, et al. (2014) A primer of statistical methods for correlating parameters and properties of electrospun poly( l -lactide) scaffolds for tissue engineering-PART 2: Regression . J Biomed Mater Res 103: 103–114. Available: http://dx.doi.org/10.1002/jbm.a.35183.en
dc.identifier.issn1549-3296en
dc.identifier.pmid24668730en
dc.identifier.doi10.1002/jbm.a.35183en
dc.identifier.urihttp://hdl.handle.net/10754/594264en
dc.description.abstractThis two-articles series presents an in-depth discussion of electrospun poly-l-lactide scaffolds for tissue engineering by means of statistical methodologies that can be used, in general, to gain a quantitative and systematic insight about effects and interactions between a handful of key scaffold properties (Ys) and a set of process parameters (Xs) in electrospinning. While Part-1 dealt with the DOE methods to unveil the interactions between Xs in determining the morphomechanical properties (ref. Y1-4), this Part-2 article continues and refocuses the discussion on the interdependence of scaffold properties investigated by standard regression methods. The discussion first explores the connection between mechanical properties (Y4) and morphological descriptors of the scaffolds (Y1-3) in 32 types of scaffolds, finding that the mean fiber diameter (Y1) plays a predominant role which is nonetheless and crucially modulated by the molecular weight (MW) of PLLA. The second part examines the biological performance (Y5) (i.e. the cell proliferation of seeded bone marrow-derived mesenchymal stromal cells) on a random subset of eight scaffolds vs. the mechanomorphological properties (Y1-4). In this case, the featured regression analysis on such an incomplete set was not conclusive, though, indirectly suggesting in quantitative terms that cell proliferation could not fully be explained as a function of considered mechanomorphological properties (Y1-4), but in the early stage seeding, and that a randomization effects occurs over time such that the differences in initial cell proliferation performance (at day 1) is smeared over time. The findings may be the cornerstone of a novel route to accrue sufficient understanding and establish design rules for scaffold biofunctional vs. architecture, mechanical properties, and process parameters.en
dc.publisherWiley-Blackwellen
dc.subjectbiomaterialsen
dc.subjectelectrospinningen
dc.subjectmechanical propertiesen
dc.subjectparametric studyen
dc.subjectprocess controlen
dc.subjectstructure-property relationsen
dc.titleA primer of statistical methods for correlating parameters and properties of electrospun poly( l -lactide) scaffolds for tissue engineering-PART 2: Regressionen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalJournal of Biomedical Materials Research Part Aen
dc.contributor.institutionDepartment of Chemical Science and Technology and NAST Center; University of Rome Tor Vergata; Rome Italyen
dc.contributor.institutionTissue Engineering Laboratory, CIR-Center of Integrated Research, Università Campus Bio-Medico di Roma; Rome Italyen
dc.contributor.institutionENEA,CR Casaccia; Via Anguillarese 301, Santa Maria di Galeria Rome Italyen
dc.contributor.institutionInternational Research Center for Mathematics & Mechanics of Complex Systems, University of L'Aquila; Via S. Pasquale, Cisterna di Latina (LT) Italyen
kaust.authorLicoccia, Silviaen
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