Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant

Handle URI:
http://hdl.handle.net/10754/594233
Title:
Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant
Authors:
Hudik, Elodie; Yoshioka, Yasushi; Domenichini, SéVerine; Bourge, Mickaël; Soubigout-Taconnat, Ludivine; Mazubert, Christelle; Yi, Dalong; Bujaldon, Sandrine; Hayashi, Hiroyuki; De Veylder, Lieven; Bergounioux, Catherine; Benhamed, Moussa ( 0000-0002-4181-1702 ) ; Raynaud, Cécile
Abstract:
The majority of research on cell cycle regulation is focused on the nuclear events that govern the replication and segregation of the genome between the two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, and coordination among these organelles is required for proper cell cycle progression, as evidenced by the isolation of several mutants in which both organelle function and overall plant development were affected. To investigate how chloroplast dysfunction affects the cell cycle, we analyzed the crumpled leaf (crl) mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for a chloroplastic protein and displays particularly severe developmental defects. In the crl mutant, we reveal that cell cycle regulation is altered drastically and that meristematic cells prematurely enter differentiation, leading to reduced plant stature and early endoreduplication in the leaves. This response is due to the repression of several key cell cycle regulators as well as constitutive activation of stress-response genes, among them the cell cycle inhibitor SIAMESE-RELATED5. One unique feature of the crl mutant is that it produces aplastidic cells in several organs, including the root tip. By investigating the consequence of the absence of plastids on cell cycle progression, we showed that nuclear DNA replication occurs in aplastidic cells in the root tip, which opens future research prospects regarding the dialogue between plastids and the nucleus during cell cycle regulation in higher plants.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Hudik E, Yoshioka Y, Domenichini S, Bourge M, Soubigout-Taconnat L, et al. (2014) Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant . Plant Physiol 166: 152–167. Available: http://dx.doi.org/10.1104/pp.114.242628.
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
Plant Physiology
Issue Date:
18-Jul-2014
DOI:
10.1104/pp.114.242628
PubMed ID:
25037213
PubMed Central ID:
PMC4149703
Type:
Article
ISSN:
0032-0889; 1532-2548
Sponsors:
This work was supported by the Agence Nationale de la Recherche of France (grant no. ANR 2010 JCJC1207 01) and the Interuniversity Attraction Poles Program (grant no. IUAP P7/29 MARS), initiated by the Belgian Science Policy Office, as well as by the facilities and expertise of the Imagif Cell Biology Unit of the Gif Campus, which is supported by the Infrastructures en Biologie Sante et Agronomie, the French National Research Agency, under Investments for the Future programs France-BioImaging Infrastructure (grant no. ANR-10-INSB-04-01), Saclay Plant Sciences (grant no. ANR-10-LABX-0040-SPS), and the Conseil General de l'Essonne.
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorHudik, Elodieen
dc.contributor.authorYoshioka, Yasushien
dc.contributor.authorDomenichini, SéVerineen
dc.contributor.authorBourge, Mickaëlen
dc.contributor.authorSoubigout-Taconnat, Ludivineen
dc.contributor.authorMazubert, Christelleen
dc.contributor.authorYi, Dalongen
dc.contributor.authorBujaldon, Sandrineen
dc.contributor.authorHayashi, Hiroyukien
dc.contributor.authorDe Veylder, Lievenen
dc.contributor.authorBergounioux, Catherineen
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorRaynaud, Cécileen
dc.date.accessioned2016-01-19T14:43:58Zen
dc.date.available2016-01-19T14:43:58Zen
dc.date.issued2014-07-18en
dc.identifier.citationHudik E, Yoshioka Y, Domenichini S, Bourge M, Soubigout-Taconnat L, et al. (2014) Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant . Plant Physiol 166: 152–167. Available: http://dx.doi.org/10.1104/pp.114.242628.en
dc.identifier.issn0032-0889en
dc.identifier.issn1532-2548en
dc.identifier.pmid25037213en
dc.identifier.doi10.1104/pp.114.242628en
dc.identifier.urihttp://hdl.handle.net/10754/594233en
dc.description.abstractThe majority of research on cell cycle regulation is focused on the nuclear events that govern the replication and segregation of the genome between the two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, and coordination among these organelles is required for proper cell cycle progression, as evidenced by the isolation of several mutants in which both organelle function and overall plant development were affected. To investigate how chloroplast dysfunction affects the cell cycle, we analyzed the crumpled leaf (crl) mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for a chloroplastic protein and displays particularly severe developmental defects. In the crl mutant, we reveal that cell cycle regulation is altered drastically and that meristematic cells prematurely enter differentiation, leading to reduced plant stature and early endoreduplication in the leaves. This response is due to the repression of several key cell cycle regulators as well as constitutive activation of stress-response genes, among them the cell cycle inhibitor SIAMESE-RELATED5. One unique feature of the crl mutant is that it produces aplastidic cells in several organs, including the root tip. By investigating the consequence of the absence of plastids on cell cycle progression, we showed that nuclear DNA replication occurs in aplastidic cells in the root tip, which opens future research prospects regarding the dialogue between plastids and the nucleus during cell cycle regulation in higher plants.en
dc.description.sponsorshipThis work was supported by the Agence Nationale de la Recherche of France (grant no. ANR 2010 JCJC1207 01) and the Interuniversity Attraction Poles Program (grant no. IUAP P7/29 MARS), initiated by the Belgian Science Policy Office, as well as by the facilities and expertise of the Imagif Cell Biology Unit of the Gif Campus, which is supported by the Infrastructures en Biologie Sante et Agronomie, the French National Research Agency, under Investments for the Future programs France-BioImaging Infrastructure (grant no. ANR-10-INSB-04-01), Saclay Plant Sciences (grant no. ANR-10-LABX-0040-SPS), and the Conseil General de l'Essonne.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.titleChloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutanten
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlant Physiologyen
dc.identifier.pmcidPMC4149703en
dc.contributor.institutionInstitut de Biologie des Plantes, Unité Mixte de Recherche 8618, Centre National de la Recherche Scientifique Université-Paris Sud, Laboratoire d’Excellence Saclay Plant ScienceOrsay, Franceen
dc.contributor.institutionDivision of Biological Science, Graduate School of Science, Nagoya UniversityChikusa-ku, Nagoya, Japanen
dc.contributor.institutionFédération de Recherche de Gif FRC3115, Pôle de Biologie CellulaireGif-sur-Yvette, Franceen
dc.contributor.institutionUnité de Recherche en Génomique VégétaleEvry, Franceen
dc.contributor.institutionDepartment of Plant Systems Biology, Vlaams Instituut voor BiotechnologieGhent, Belgiumen
dc.contributor.institutionDepartment of Plant Biotechnology and Bioinformatics, Ghent UniversityGhent, Belgiumen
dc.contributor.institutionCentre National de la Recherche Scientifique, Unité Mixte de Recherche 7141, Laboratoire de Physiologie Membranaire et Moléculaire du Chloroplaste,Institut de Biologie Physico-ChimiqueParis, Franceen
dc.contributor.institutionDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of TokyoBunkyo-ku, Tokyo, Japanen
kaust.authorHudik, Elodieen
kaust.authorBenhamed, Moussaen

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