Effects of selenium oxyanions on the white-rot fungus Phanerochaete chrysosporium

Handle URI:
http://hdl.handle.net/10754/594219
Title:
Effects of selenium oxyanions on the white-rot fungus Phanerochaete chrysosporium
Authors:
Espinosa-Ortiz, Erika J.; Gonzalez-Gil, Graciela; Saikaly, Pascal ( 0000-0001-7678-3986 ) ; van Hullebusch, Eric D.; Lens, Piet N L
Abstract:
The ability of Phanerochaete chrysosporium to reduce the oxidized forms of selenium, selenate and selenite, and their effects on the growth, substrate consumption rate, and pellet morphology of the fungus were assessed. The effect of different operational parameters (pH, glucose, and selenium concentration) on the response of P. chrysosporium to selenium oxyanions was explored as well. This fungal species showed a high sensitivity to selenium, particularly selenite, which inhibited the fungal growth and substrate consumption when supplied at 10 mg L−1 in the growth medium, whereas selenate did not have such a strong influence on the fungus. Biological removal of selenite was achieved under semi-acidic conditions (pH 4.5) with about 40 % removal efficiency, whereas less than 10 % selenium removal was achieved for incubations with selenate. P. chrysosporium was found to be a selenium-reducing organism, capable of synthesizing elemental selenium from selenite but not from selenate. Analysis with transmission electron microscopy, electron energy loss spectroscopy, and a 3D reconstruction showed that elemental selenium was produced intracellularly as nanoparticles in the range of 30–400 nm. Furthermore, selenite influenced the pellet morphology of P. chrysosporium by reducing the size of the fungal pellets and inducing their compaction and smoothness.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC)
Citation:
Espinosa-Ortiz EJ, Gonzalez-Gil G, Saikaly PE, van Hullebusch ED, Lens PNL (2014) Effects of selenium oxyanions on the white-rot fungus Phanerochaete chrysosporium. Applied Microbiology and Biotechnology 99: 2405–2418. Available: http://dx.doi.org/10.1007/s00253-014-6127-3.
Publisher:
Springer Science + Business Media
Journal:
Applied Microbiology and Biotechnology
Issue Date:
24-Oct-2014
DOI:
10.1007/s00253-014-6127-3
PubMed ID:
25341399
Type:
Article
ISSN:
0175-7598; 1432-0614
Sponsors:
The authors thank the EU for providing financial support through the Erasmus Mundus Joint Doctorate Programme ETeCoS<SUP>3</SUP> (Environmental Technologies for Contaminated Solids, Soils and Sediments, grant agreement FPA no. 2010-0009). This work was partly supported by a Global Research Partnership-Collaborative Fellows Award (GRFP-CF-2011-13-P) from the King Abdullah University of Science and Technology. The authors thank Rachid Sougrad for his assistance with TEM 3D image reconstruction.
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorEspinosa-Ortiz, Erika J.en
dc.contributor.authorGonzalez-Gil, Gracielaen
dc.contributor.authorSaikaly, Pascalen
dc.contributor.authorvan Hullebusch, Eric D.en
dc.contributor.authorLens, Piet N Len
dc.date.accessioned2016-01-19T14:43:36Zen
dc.date.available2016-01-19T14:43:36Zen
dc.date.issued2014-10-24en
dc.identifier.citationEspinosa-Ortiz EJ, Gonzalez-Gil G, Saikaly PE, van Hullebusch ED, Lens PNL (2014) Effects of selenium oxyanions on the white-rot fungus Phanerochaete chrysosporium. Applied Microbiology and Biotechnology 99: 2405–2418. Available: http://dx.doi.org/10.1007/s00253-014-6127-3.en
dc.identifier.issn0175-7598en
dc.identifier.issn1432-0614en
dc.identifier.pmid25341399en
dc.identifier.doi10.1007/s00253-014-6127-3en
dc.identifier.urihttp://hdl.handle.net/10754/594219en
dc.description.abstractThe ability of Phanerochaete chrysosporium to reduce the oxidized forms of selenium, selenate and selenite, and their effects on the growth, substrate consumption rate, and pellet morphology of the fungus were assessed. The effect of different operational parameters (pH, glucose, and selenium concentration) on the response of P. chrysosporium to selenium oxyanions was explored as well. This fungal species showed a high sensitivity to selenium, particularly selenite, which inhibited the fungal growth and substrate consumption when supplied at 10 mg L−1 in the growth medium, whereas selenate did not have such a strong influence on the fungus. Biological removal of selenite was achieved under semi-acidic conditions (pH 4.5) with about 40 % removal efficiency, whereas less than 10 % selenium removal was achieved for incubations with selenate. P. chrysosporium was found to be a selenium-reducing organism, capable of synthesizing elemental selenium from selenite but not from selenate. Analysis with transmission electron microscopy, electron energy loss spectroscopy, and a 3D reconstruction showed that elemental selenium was produced intracellularly as nanoparticles in the range of 30–400 nm. Furthermore, selenite influenced the pellet morphology of P. chrysosporium by reducing the size of the fungal pellets and inducing their compaction and smoothness.en
dc.description.sponsorshipThe authors thank the EU for providing financial support through the Erasmus Mundus Joint Doctorate Programme ETeCoS<SUP>3</SUP> (Environmental Technologies for Contaminated Solids, Soils and Sediments, grant agreement FPA no. 2010-0009). This work was partly supported by a Global Research Partnership-Collaborative Fellows Award (GRFP-CF-2011-13-P) from the King Abdullah University of Science and Technology. The authors thank Rachid Sougrad for his assistance with TEM 3D image reconstruction.en
dc.publisherSpringer Science + Business Mediaen
dc.subjectFungal pelletsen
dc.subjectPhanerochaete chrysosporiumen
dc.subjectSelenium nanoparticlesen
dc.subjectSelenium removalen
dc.titleEffects of selenium oxyanions on the white-rot fungus Phanerochaete chrysosporiumen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalApplied Microbiology and Biotechnologyen
dc.contributor.institutionUNESCO-IHE Institute for Water Education, P.O. Box 3015Delft, Netherlandsen
dc.contributor.institutionLaboratoire Géomatériaux et Environnement (EA 4508), UPEM, Université Paris-EstMarne-la-Vallée, Franceen
kaust.authorGonzalez-Gil, Gracielaen
kaust.authorSaikaly, Pascalen

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