Diversity of methanogens and sulfate-reducing bacteria in the interfaces of five deep-sea anoxic brines of the Red Sea

Handle URI:
http://hdl.handle.net/10754/594182
Title:
Diversity of methanogens and sulfate-reducing bacteria in the interfaces of five deep-sea anoxic brines of the Red Sea
Authors:
Guan, Yue ( 0000-0002-5977-6984 ) ; Hikmawan, Tyas; Antunes, Andre ( 0000-0001-7668-9842 ) ; Ngugi, David ( 0000-0002-0442-4279 ) ; Stingl, Ulrich ( 0000-0002-0684-2597 )
Abstract:
Oceanic deep hypersaline anoxic basins (DHABs) are characterized by drastic changes in physico-chemical conditions in the transition from overlaying seawater to brine body. Brine-seawater interfaces (BSIs) of several DHABs across the Mediterranean Sea have been shown to possess methanogenic and sulfate-reducing activities, yet no systematic studies have been conducted to address the potential functional diversity of methanogenic and sulfate-reducing communities in the Red Sea DHABs. Here, we evaluated the relative abundance of Bacteria and Archaea using quantitative PCR and conducted phylogenetic analyses of nearly full-length 16S rRNA genes as well as functional marker genes encoding the alpha subunits of methyl-coenzyme M reductase (mcrA) and dissimilatory sulfite reductase (dsrA). Bacteria predominated over Archaea in most locations, the majority of which were affiliated with Deltaproteobacteria, while Thaumarchaeota were the most prevalent Archaea in all sampled locations. The upper convective layers of Atlantis II Deep, which bear increasingly harsh environmental conditions, were dominated by members of the class Thermoplasmata (Marine Benthic Group E and Mediterranean Sea Brine Lakes Group 1). Our study revealed unique microbial compositions, the presence of niche-specific groups, and collectively, a higher diversity of sulfate-reducing communities compared to methanogenic communities in all five studied locations. © 2015 Institut Pasteur.
KAUST Department:
Red Sea Research Center (RSRC); Computational Bioscience Research Center (CBRC)
Citation:
Guan Y, Hikmawan T, Antunes A, Ngugi D, Stingl U (2015) Diversity of methanogens and sulfate-reducing bacteria in the interfaces of five deep-sea anoxic brines of the Red Sea. Research in Microbiology 166: 688–699. Available: http://dx.doi.org/10.1016/j.resmic.2015.07.002.
Publisher:
Elsevier BV
Journal:
Research in Microbiology
Issue Date:
Nov-2015
DOI:
10.1016/j.resmic.2015.07.002
PubMed ID:
26192212
Type:
Article
ISSN:
0923-2508
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGuan, Yueen
dc.contributor.authorHikmawan, Tyasen
dc.contributor.authorAntunes, Andreen
dc.contributor.authorNgugi, Daviden
dc.contributor.authorStingl, Ulrichen
dc.date.accessioned2016-01-19T13:23:20Zen
dc.date.available2016-01-19T13:23:20Zen
dc.date.issued2015-11en
dc.identifier.citationGuan Y, Hikmawan T, Antunes A, Ngugi D, Stingl U (2015) Diversity of methanogens and sulfate-reducing bacteria in the interfaces of five deep-sea anoxic brines of the Red Sea. Research in Microbiology 166: 688–699. Available: http://dx.doi.org/10.1016/j.resmic.2015.07.002.en
dc.identifier.issn0923-2508en
dc.identifier.pmid26192212en
dc.identifier.doi10.1016/j.resmic.2015.07.002en
dc.identifier.urihttp://hdl.handle.net/10754/594182en
dc.description.abstractOceanic deep hypersaline anoxic basins (DHABs) are characterized by drastic changes in physico-chemical conditions in the transition from overlaying seawater to brine body. Brine-seawater interfaces (BSIs) of several DHABs across the Mediterranean Sea have been shown to possess methanogenic and sulfate-reducing activities, yet no systematic studies have been conducted to address the potential functional diversity of methanogenic and sulfate-reducing communities in the Red Sea DHABs. Here, we evaluated the relative abundance of Bacteria and Archaea using quantitative PCR and conducted phylogenetic analyses of nearly full-length 16S rRNA genes as well as functional marker genes encoding the alpha subunits of methyl-coenzyme M reductase (mcrA) and dissimilatory sulfite reductase (dsrA). Bacteria predominated over Archaea in most locations, the majority of which were affiliated with Deltaproteobacteria, while Thaumarchaeota were the most prevalent Archaea in all sampled locations. The upper convective layers of Atlantis II Deep, which bear increasingly harsh environmental conditions, were dominated by members of the class Thermoplasmata (Marine Benthic Group E and Mediterranean Sea Brine Lakes Group 1). Our study revealed unique microbial compositions, the presence of niche-specific groups, and collectively, a higher diversity of sulfate-reducing communities compared to methanogenic communities in all five studied locations. © 2015 Institut Pasteur.en
dc.publisherElsevier BVen
dc.subjectBiodiversityen
dc.subjectBrine poolsen
dc.subjectHypersaline environmentsen
dc.subjectMethanogensen
dc.subjectSulfate reducersen
dc.titleDiversity of methanogens and sulfate-reducing bacteria in the interfaces of five deep-sea anoxic brines of the Red Seaen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalResearch in Microbiologyen
kaust.authorGuan, Yueen
kaust.authorHikmawan, Tyas I.en
kaust.authorAntunes, Andreen
kaust.authorNgugi, Daviden
kaust.authorStingl, Ulrichen

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