Replisome speed determines the efficiency of the Tus−Ter replication termination barrier

Handle URI:
http://hdl.handle.net/10754/594133
Title:
Replisome speed determines the efficiency of the Tus−Ter replication termination barrier
Authors:
Elshenawy, Mohamed ( 0000-0002-8599-8388 ) ; Jergic, Slobodan; Xu, Zhi Qiang; Sobhy, Mohamed Abdelmaboud; Takahashi, Masateru; Oakley, Aaron J.; Dixon, Nicholas E.; Hamdan, Samir ( 0000-0001-5192-1852 )
Abstract:
In all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites. Escherichia coli sets a 'replication fork trap' that allows the first arriving fork to enter but not to leave the terminus region. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction. However, the efficiency of fork blockage by Tus-Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus-Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes. ©2015 Macmillan Publishers Limited. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Elshenawy MM, Jergic S, Xu Z-Q, Sobhy MA, Takahashi M, et al. (2015) Replisome speed determines the efficiency of the Tus−Ter replication termination barrier. Nature 525: 394–398. Available: http://dx.doi.org/10.1038/nature14866.
Publisher:
Nature Publishing Group
Journal:
Nature
Issue Date:
31-Aug-2015
DOI:
10.1038/nature14866
PubMed ID:
26322585
Type:
Article
ISSN:
0028-0836; 1476-4687
Sponsors:
DP0877658, ARC, Australian Research Council; DP0984797, ARC, Australian Research Council; KAUST, Australian Research Council; Australian Research Council
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorElshenawy, Mohameden
dc.contributor.authorJergic, Slobodanen
dc.contributor.authorXu, Zhi Qiangen
dc.contributor.authorSobhy, Mohamed Abdelmabouden
dc.contributor.authorTakahashi, Masateruen
dc.contributor.authorOakley, Aaron J.en
dc.contributor.authorDixon, Nicholas E.en
dc.contributor.authorHamdan, Samiren
dc.date.accessioned2016-01-19T13:22:22Zen
dc.date.available2016-01-19T13:22:22Zen
dc.date.issued2015-08-31en
dc.identifier.citationElshenawy MM, Jergic S, Xu Z-Q, Sobhy MA, Takahashi M, et al. (2015) Replisome speed determines the efficiency of the Tus−Ter replication termination barrier. Nature 525: 394–398. Available: http://dx.doi.org/10.1038/nature14866.en
dc.identifier.issn0028-0836en
dc.identifier.issn1476-4687en
dc.identifier.pmid26322585en
dc.identifier.doi10.1038/nature14866en
dc.identifier.urihttp://hdl.handle.net/10754/594133en
dc.description.abstractIn all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites. Escherichia coli sets a 'replication fork trap' that allows the first arriving fork to enter but not to leave the terminus region. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction. However, the efficiency of fork blockage by Tus-Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus-Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes. ©2015 Macmillan Publishers Limited. All rights reserved.en
dc.description.sponsorshipDP0877658, ARC, Australian Research Council; DP0984797, ARC, Australian Research Council; KAUST, Australian Research Council; Australian Research Councilen
dc.publisherNature Publishing Groupen
dc.titleReplisome speed determines the efficiency of the Tus−Ter replication termination barrieren
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalNatureen
dc.contributor.institutionCentre for Medical and Molecular Bioscience, Illawarra Health and Medical Research Institute, University of WollongongNSW, Australiaen
kaust.authorElshenawy, Mohameden
kaust.authorSobhy, Mohamed Abdelmabouden
kaust.authorTakahashi, Masateruen
kaust.authorHamdan, Samiren

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