Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives

Handle URI:
http://hdl.handle.net/10754/594061
Title:
Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives
Authors:
Mahfouz, Magdy M. ( 0000-0002-0616-6365 ) ; Piatek, Agnieszka Anna ( 0000-0001-9587-6360 ) ; Stewart, Charles Neal
Abstract:
The ability to precisely modify genome sequence and regulate gene expression patterns in a site-specific manner holds much promise in plant biotechnology. Genome-engineering technologies that enable such highly specific and efficient modification are advancing with unprecedented pace. Transcription activator-like effectors (TALEs) provide customizable DNA-binding modules designed to bind to any sequence of interest. Thus, TALEs have been used as a DNA targeting module fused to functional domains for a variety of targeted genomic and epigenomic modifications. TALE nucleases (TALENs) have been used with much success across eukaryotic species to edit genomes. Recently, clustered regularly interspaced palindromic repeats (CRISPRs) that are used as guide RNAs for Cas9 nuclease-specific digestion has been introduced as a highly efficient DNA-targeting platform for genome editing and regulation. Here, we review the discovery, development and limitations of TALENs and CRIPSR/Cas9 systems as genome-engineering platforms in plants. We discuss the current questions, potential improvements and the development of the next-generation genome-editing platforms with an emphasis on producing designer plants to address the needs of agriculture and basic plant biology.
KAUST Department:
Center for Desert Agriculture
Citation:
Mahfouz MM, Piatek A, Stewart CN (2014) Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives. Plant Biotechnology Journal 12: 1006–1014. Available: http://dx.doi.org/10.1111/pbi.12256.
Publisher:
Wiley-Blackwell
Journal:
Plant Biotechnology Journal
Issue Date:
24-Sep-2014
DOI:
10.1111/pbi.12256
PubMed ID:
25250853
Type:
Article
ISSN:
1467-7644
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorMahfouz, Magdy M.en
dc.contributor.authorPiatek, Agnieszka Annaen
dc.contributor.authorStewart, Charles Nealen
dc.date.accessioned2016-01-19T13:20:34Zen
dc.date.available2016-01-19T13:20:34Zen
dc.date.issued2014-09-24en
dc.identifier.citationMahfouz MM, Piatek A, Stewart CN (2014) Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives. Plant Biotechnology Journal 12: 1006–1014. Available: http://dx.doi.org/10.1111/pbi.12256.en
dc.identifier.issn1467-7644en
dc.identifier.pmid25250853en
dc.identifier.doi10.1111/pbi.12256en
dc.identifier.urihttp://hdl.handle.net/10754/594061en
dc.description.abstractThe ability to precisely modify genome sequence and regulate gene expression patterns in a site-specific manner holds much promise in plant biotechnology. Genome-engineering technologies that enable such highly specific and efficient modification are advancing with unprecedented pace. Transcription activator-like effectors (TALEs) provide customizable DNA-binding modules designed to bind to any sequence of interest. Thus, TALEs have been used as a DNA targeting module fused to functional domains for a variety of targeted genomic and epigenomic modifications. TALE nucleases (TALENs) have been used with much success across eukaryotic species to edit genomes. Recently, clustered regularly interspaced palindromic repeats (CRISPRs) that are used as guide RNAs for Cas9 nuclease-specific digestion has been introduced as a highly efficient DNA-targeting platform for genome editing and regulation. Here, we review the discovery, development and limitations of TALENs and CRIPSR/Cas9 systems as genome-engineering platforms in plants. We discuss the current questions, potential improvements and the development of the next-generation genome-editing platforms with an emphasis on producing designer plants to address the needs of agriculture and basic plant biology.en
dc.publisherWiley-Blackwellen
dc.subjectCRISPR/Cas9 systemen
dc.subjectDesigner transcriptional regulatorsen
dc.subjectDNA-binding modulesen
dc.subjectSynthetic site-specific nucleases (SNNs)en
dc.subjectTAL effectorsen
dc.subjectTargeted genome modificationsen
dc.titleGenome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectivesen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalPlant Biotechnology Journalen
dc.contributor.institutionDepartment of Plant Sciences; University of Tennessee; Knoxville TN USAen
kaust.authorMahfouz, Magdy M.en
kaust.authorPiatek, Agnieszka Annaen

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