Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea

Handle URI:
http://hdl.handle.net/10754/593685
Title:
Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea
Authors:
Mwirichia, Romano; Alam, Intikhab; Rashid, Mamoon ( 0000-0003-4851-4994 ) ; Vinu, Manikandan; Ba Alawi, Wail ( 0000-0002-2747-4703 ) ; Anthony Kamau, Allan; Ngugi, David ( 0000-0002-0442-4279 ) ; Göker, Markus; Klenk, Hans-Peter; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Stingl, Ulrich ( 0000-0002-0684-2597 )
Abstract:
The candidate Division MSBL1 (Mediterranean Sea Brine Lakes 1) comprises a monophyletic group of uncultured archaea found in different hypersaline environments. Previous studies propose methanogenesis as the main metabolism. Here, we describe a metabolic reconstruction of MSBL1 based on 32 single-cell amplified genomes from Brine Pools of the Red Sea (Atlantis II, Discovery, Nereus, Erba and Kebrit). Phylogeny based on rRNA genes as well as conserved single copy genes delineates the group as a putative novel lineage of archaea. Our analysis shows that MSBL1 may ferment glucose via the Embden–Meyerhof–Parnas pathway. However, in the absence of organic carbon, carbon dioxide may be fixed via the ribulose bisphosphate carboxylase, Wood-Ljungdahl pathway or reductive TCA cycle. Therefore, based on the occurrence of genes for glycolysis, absence of the core genes found in genomes of all sequenced methanogens and the phylogenetic position, we hypothesize that the MSBL1 are not methanogens, but probably sugar-fermenting organisms capable of autotrophic growth. Such a mixotrophic lifestyle would confer survival advantage (or possibly provide a unique narrow niche) when glucose and other fermentable sugars are not available.
KAUST Department:
Red Sea Research Center (RSRC); Computational Bioscience Research Center (CBRC)
Citation:
Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea 2016, 6:19181 Scientific Reports
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
13-Jan-2016
DOI:
10.1038/srep19181
Type:
Article
ISSN:
2045-2322
Additional Links:
http://www.nature.com/articles/srep19181
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorMwirichia, Romanoen
dc.contributor.authorAlam, Intikhaben
dc.contributor.authorRashid, Mamoonen
dc.contributor.authorVinu, Manikandanen
dc.contributor.authorBa Alawi, Wailen
dc.contributor.authorAnthony Kamau, Allanen
dc.contributor.authorNgugi, Daviden
dc.contributor.authorGöker, Markusen
dc.contributor.authorKlenk, Hans-Peteren
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorStingl, Ulrichen
dc.date.accessioned2016-01-18T08:27:45Zen
dc.date.available2016-01-18T08:27:45Zen
dc.date.issued2016-01-13en
dc.identifier.citationMetabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Sea 2016, 6:19181 Scientific Reportsen
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep19181en
dc.identifier.urihttp://hdl.handle.net/10754/593685en
dc.description.abstractThe candidate Division MSBL1 (Mediterranean Sea Brine Lakes 1) comprises a monophyletic group of uncultured archaea found in different hypersaline environments. Previous studies propose methanogenesis as the main metabolism. Here, we describe a metabolic reconstruction of MSBL1 based on 32 single-cell amplified genomes from Brine Pools of the Red Sea (Atlantis II, Discovery, Nereus, Erba and Kebrit). Phylogeny based on rRNA genes as well as conserved single copy genes delineates the group as a putative novel lineage of archaea. Our analysis shows that MSBL1 may ferment glucose via the Embden–Meyerhof–Parnas pathway. However, in the absence of organic carbon, carbon dioxide may be fixed via the ribulose bisphosphate carboxylase, Wood-Ljungdahl pathway or reductive TCA cycle. Therefore, based on the occurrence of genes for glycolysis, absence of the core genes found in genomes of all sequenced methanogens and the phylogenetic position, we hypothesize that the MSBL1 are not methanogens, but probably sugar-fermenting organisms capable of autotrophic growth. Such a mixotrophic lifestyle would confer survival advantage (or possibly provide a unique narrow niche) when glucose and other fermentable sugars are not available.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/articles/srep19181en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleMetabolic traits of an uncultured archaeal lineage -MSBL1- from brine pools of the Red Seaen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionEmbu University College, Embu, Kenyaen
dc.contributor.institutionGerman Collection for Microorganisms and Cell Cultures GmbH (DSMZ), Inhoffenstraße 7b, 38124 Braunschweig, Germanyen
dc.contributor.institutionSchool of Biology, Newcastle University, Newcastle upon Tyne, United Kingdomen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorMwirichia, Romanoen
kaust.authorAlam, Intikhaben
kaust.authorRashid, Mamoonen
kaust.authorVinu, Manikandanen
kaust.authorBa Alawi, Wailen
kaust.authorKamau, Allanen
kaust.authorNgugi, Daviden
kaust.authorBajic, Vladimir B.en
kaust.authorStingl, Ulrichen
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