Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula

Handle URI:
http://hdl.handle.net/10754/593396
Title:
Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula
Authors:
Kamphuis, L. G.; Lichtenzveig, J.; Peng, K.; Guo, S.-M.; Klingler, John ( 0000-0003-3951-9734 ) ; Siddique, K. H. M.; Gao, L.-L.; Singh, K. B.
Abstract:
Aphids cause significant yield losses in agricultural crops worldwide. Medicago truncatula, a model legume, cultivated pasture species in Australia and close relative of alfalfa (Medicago sativa), was used to study the defence response against Therioaphis trifolii f. maculate [spotted alfalfa aphid (SAA)]. Aphid performance and plant damage were compared among three accessions. A20 is highly susceptible, A17 has moderate resistance, and Jester is strongly resistant. Subsequent analyses using A17 and A20, reciprocal F1s and an A17×A20 recombinant inbred line (RIL) population revealed that this moderate resistance is phloem mediated and involves antibiosis and tolerance but not antixenosis. Electrical penetration graph analysis also identified a novel waveform termed extended potential drop, which occurred following SAA infestation of M. truncatula. Genetic dissection using the RIL population revealed three quantitative trait loci on chromosomes 3, 6, and 7 involved in distinct modes of aphid defence including antibiosis and tolerance. An antibiosis locus resides on linkage group 3 (LG3) and is derived from A17, whereas a plant tolerance and antibiosis locus resides on LG6 and is derived from A20, which exhibits strong temporary tolerance. The loci identified reside in regions harbouring classical resistance genes, and introgression of these loci in current medic cultivars may help provide durable resistance to SAA, while elucidation of their molecular mechanisms may provide valuable insight into other aphid–plant interactions.
KAUST Department:
Desert Agriculture Initiative
Citation:
Characterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula 2013, 64 (16):5157 Journal of Experimental Botany
Publisher:
Oxford University Press (OUP)
Journal:
Journal of Experimental Botany
Issue Date:
21-Sep-2013
DOI:
10.1093/jxb/ert305
Type:
Article
ISSN:
0022-0957; 1460-2431
Additional Links:
http://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/ert305
Appears in Collections:
Articles; Desert Agriculture Initiative

Full metadata record

DC FieldValue Language
dc.contributor.authorKamphuis, L. G.en
dc.contributor.authorLichtenzveig, J.en
dc.contributor.authorPeng, K.en
dc.contributor.authorGuo, S.-M.en
dc.contributor.authorKlingler, Johnen
dc.contributor.authorSiddique, K. H. M.en
dc.contributor.authorGao, L.-L.en
dc.contributor.authorSingh, K. B.en
dc.date.accessioned2016-01-13T14:36:35Zen
dc.date.available2016-01-13T14:36:35Zen
dc.date.issued2013-09-21en
dc.identifier.citationCharacterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatula 2013, 64 (16):5157 Journal of Experimental Botanyen
dc.identifier.issn0022-0957en
dc.identifier.issn1460-2431en
dc.identifier.doi10.1093/jxb/ert305en
dc.identifier.urihttp://hdl.handle.net/10754/593396en
dc.description.abstractAphids cause significant yield losses in agricultural crops worldwide. Medicago truncatula, a model legume, cultivated pasture species in Australia and close relative of alfalfa (Medicago sativa), was used to study the defence response against Therioaphis trifolii f. maculate [spotted alfalfa aphid (SAA)]. Aphid performance and plant damage were compared among three accessions. A20 is highly susceptible, A17 has moderate resistance, and Jester is strongly resistant. Subsequent analyses using A17 and A20, reciprocal F1s and an A17×A20 recombinant inbred line (RIL) population revealed that this moderate resistance is phloem mediated and involves antibiosis and tolerance but not antixenosis. Electrical penetration graph analysis also identified a novel waveform termed extended potential drop, which occurred following SAA infestation of M. truncatula. Genetic dissection using the RIL population revealed three quantitative trait loci on chromosomes 3, 6, and 7 involved in distinct modes of aphid defence including antibiosis and tolerance. An antibiosis locus resides on linkage group 3 (LG3) and is derived from A17, whereas a plant tolerance and antibiosis locus resides on LG6 and is derived from A20, which exhibits strong temporary tolerance. The loci identified reside in regions harbouring classical resistance genes, and introgression of these loci in current medic cultivars may help provide durable resistance to SAA, while elucidation of their molecular mechanisms may provide valuable insight into other aphid–plant interactions.en
dc.language.isoenen
dc.publisherOxford University Press (OUP)en
dc.relation.urlhttp://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/ert305en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectAntibiosisen
dc.subjectantixenosisen
dc.subjectEPGen
dc.subjectherbivoryen
dc.subjectphloemen
dc.subjectquantitative trait locus (QTL)en
dc.subjectsap-sucking insecten
dc.subjectvein chlorosisen
dc.titleCharacterization and genetic dissection of resistance to spotted alfalfa aphid (Therioaphis trifolii) in Medicago truncatulaen
dc.typeArticleen
dc.contributor.departmentDesert Agriculture Initiativeen
dc.identifier.journalJournal of Experimental Botanyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCSIRO Plant Industry, Private Bag 5, Wembley, WA 6913, Australiaen
dc.contributor.institutionThe UWA Institute of Agriculture, University of Western Australia, Crawley, WA 6009, Australiaen
dc.contributor.institutionDepartment of Environment and Agriculture, Curtin University, Bentley, WA 6102, Australiaen
dc.contributor.institutionKey Laboratory of Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, PR Chinaen
dc.contributor.institutionResearch School of Biology, Australian National University, Acton, ACT 0200, Australiaen
dc.contributor.institutionBoyce Thompson Institute for Plant Research, Ithaca, NY 14853, USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKlingler, J. P.en
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