Cytotoxicity and intracellular dissolution of nickel nanowires

Handle URI:
http://hdl.handle.net/10754/592811
Title:
Cytotoxicity and intracellular dissolution of nickel nanowires
Authors:
Perez, Jose E. ( 0000-0002-2206-0034 ) ; Contreras, Maria F. ( 0000-0001-6239-5325 ) ; Vidal, Enrique Vilanova; Felix Servin, Laura P.; Margineanu, Michael B. ( 0000-0002-9836-1802 ) ; Luongo, Giovanni; Porter, Alexandra E.; Dunlop, Iain E.; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 µm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Citation:
Cytotoxicity and intracellular dissolution of nickel nanowires 2015:1 Nanotoxicology
Publisher:
Informa Healthcare
Journal:
Nanotoxicology
Issue Date:
22-Dec-2015
DOI:
10.3109/17435390.2015.1132343
Type:
Article
ISSN:
1743-5390; 1743-5404
Is Supplemented By:
Perez, J. E., Contreras, M. F., Vilanova, E., Felix, L. P., Margineanu, M. B., Luongo, G., … Kosel, J. (2016). Cytotoxicity and intracellular dissolution of nickel nanowires. Figshare. https://doi.org/10.6084/m9.figshare.c.2169857; DOI:10.6084/M9.FIGSHARE.C.2169857; HANDLE:http://hdl.handle.net/10754/624123
Additional Links:
http://www.tandfonline.com/doi/full/10.3109/17435390.2015.1132343
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPerez, Jose E.en
dc.contributor.authorContreras, Maria F.en
dc.contributor.authorVidal, Enrique Vilanovaen
dc.contributor.authorFelix Servin, Laura P.en
dc.contributor.authorMargineanu, Michael B.en
dc.contributor.authorLuongo, Giovannien
dc.contributor.authorPorter, Alexandra E.en
dc.contributor.authorDunlop, Iain E.en
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2016-01-05T06:09:01Zen
dc.date.available2016-01-05T06:09:01Zen
dc.date.issued2015-12-22en
dc.identifier.citationCytotoxicity and intracellular dissolution of nickel nanowires 2015:1 Nanotoxicologyen
dc.identifier.issn1743-5390en
dc.identifier.issn1743-5404en
dc.identifier.doi10.3109/17435390.2015.1132343en
dc.identifier.urihttp://hdl.handle.net/10754/592811en
dc.description.abstractThe assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 µm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.en
dc.language.isoenen
dc.publisherInforma Healthcareen
dc.relation.urlhttp://www.tandfonline.com/doi/full/10.3109/17435390.2015.1132343en
dc.rightsThis is an Accepted Manuscript of an article published by Taylor & Francis in Nanotoxicology on 22 Dec 2015, available online: http://wwww.tandfonline.com/10.3109/17435390.2015.1132343.en
dc.subjectnanotoxicologyen
dc.subjectparticle charactarizationen
dc.subjectnanomedicineen
dc.subjectmagnetic nanowiresen
dc.subjectnanoparticlesen
dc.titleCytotoxicity and intracellular dissolution of nickel nanowiresen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalNanotoxicologyen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Materials, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdomen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorPerez, Jose E.en
kaust.authorContreras, Maria F.en
kaust.authorVidal, Enrique Vilanovaen
kaust.authorFelix Servin, Laura P.en
kaust.authorMargineanu, Michael B.en
kaust.authorRavasi, Timothyen
kaust.authorKosel, Jürgenen
dc.relation.isSupplementedByPerez, J. E., Contreras, M. F., Vilanova, E., Felix, L. P., Margineanu, M. B., Luongo, G., … Kosel, J. (2016). Cytotoxicity and intracellular dissolution of nickel nanowires. Figshare. https://doi.org/10.6084/m9.figshare.c.2169857en
dc.relation.isSupplementedByDOI:10.6084/M9.FIGSHARE.C.2169857en
dc.relation.isSupplementedByHANDLE:http://hdl.handle.net/10754/624123en
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