Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

Handle URI:
http://hdl.handle.net/10754/592742
Title:
Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance
Authors:
Hühn, Jonas; Fedeli, Chiara; Zhang, Qian; Masood, Atif; del Pino, Pablo; Khashab, Niveen M. ( 0000-0003-2728-0666 ) ; Papini, Emanuele; Parak, Wolfgang J.
Abstract:
Protein adsorption to nanoparticles is described as a chemical reaction in which proteins attach to binding sites on the nanoparticle surface. This process can be described with a dissociation coefficient, which tells how many proteins are adsorbed per nanoparticle in dependence of the protein concentration. Different techniques to experimentally determine dissociation coefficients of protein adsorption to nanoparticles are reviewed. Results of more than 130 experiments in which dissociation coefficients have been determined are compared. Data show that different methods, nanoparticle systems, and proteins can lead to significantly different dissociation coefficients. However, we observed a clear tendency of smaller dissociation coefficients upon less negative towards more positive zeta potentials of the nanoparticles. The zeta potential thus is a key parameter influencing protein adsorption to the surface of nanoparticles. Our analysis highlights the importance of the characterization of the parameters governing protein-nanoparticle interaction for quantitative evaluation and objective literature comparison.
KAUST Department:
Smart Hybrid Materials (SHMs) lab; Advanced Membranes and Porous Materials Research Center
Citation:
Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance 2015 The International Journal of Biochemistry & Cell Biology
Publisher:
Elsevier BV
Journal:
The International Journal of Biochemistry & Cell Biology
Issue Date:
31-Dec-2015
DOI:
10.1016/j.biocel.2015.12.015
Type:
Article
ISSN:
13572725
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S135727251530087X
Appears in Collections:
Articles; Advanced Membranes and Porous Materials Research Center; Controlled Release and Delivery Laboratory

Full metadata record

DC FieldValue Language
dc.contributor.authorHühn, Jonasen
dc.contributor.authorFedeli, Chiaraen
dc.contributor.authorZhang, Qianen
dc.contributor.authorMasood, Atifen
dc.contributor.authordel Pino, Pabloen
dc.contributor.authorKhashab, Niveen M.en
dc.contributor.authorPapini, Emanueleen
dc.contributor.authorParak, Wolfgang J.en
dc.date.accessioned2016-01-04T06:22:52Zen
dc.date.available2016-01-04T06:22:52Zen
dc.date.issued2015-12-31en
dc.identifier.citationDissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance 2015 The International Journal of Biochemistry & Cell Biologyen
dc.identifier.issn13572725en
dc.identifier.doi10.1016/j.biocel.2015.12.015en
dc.identifier.urihttp://hdl.handle.net/10754/592742en
dc.description.abstractProtein adsorption to nanoparticles is described as a chemical reaction in which proteins attach to binding sites on the nanoparticle surface. This process can be described with a dissociation coefficient, which tells how many proteins are adsorbed per nanoparticle in dependence of the protein concentration. Different techniques to experimentally determine dissociation coefficients of protein adsorption to nanoparticles are reviewed. Results of more than 130 experiments in which dissociation coefficients have been determined are compared. Data show that different methods, nanoparticle systems, and proteins can lead to significantly different dissociation coefficients. However, we observed a clear tendency of smaller dissociation coefficients upon less negative towards more positive zeta potentials of the nanoparticles. The zeta potential thus is a key parameter influencing protein adsorption to the surface of nanoparticles. Our analysis highlights the importance of the characterization of the parameters governing protein-nanoparticle interaction for quantitative evaluation and objective literature comparison.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S135727251530087Xen
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in The International Journal of Biochemistry & Cell Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in The International Journal of Biochemistry & Cell Biology, 31 December 2015. DOI: 10.1016/j.biocel.2015.12.015en
dc.titleDissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevanceen
dc.typeArticleen
dc.contributor.departmentSmart Hybrid Materials (SHMs) laben
dc.contributor.departmentAdvanced Membranes and Porous Materials Research Centeren
dc.identifier.journalThe International Journal of Biochemistry & Cell Biologyen
dc.eprint.versionPost-printen
dc.contributor.institutionFachbereich Physik, Philipps Universität Marburg, Marburg, Germanyen
dc.contributor.institutionDepartment of Biomedical Science, University of Padova, Padova, Italyen
dc.contributor.institutionCIC Biomagune, San Sebastian, Spainen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKhashab, Niveen M.en
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