Tunable Nanocarrier Morphologies from Glycopolypeptide-based Amphiphilic Biocompatible Star Copolymers and Their Carbohydrate Specific Intracellular Delivery

Handle URI:
http://hdl.handle.net/10754/592611
Title:
Tunable Nanocarrier Morphologies from Glycopolypeptide-based Amphiphilic Biocompatible Star Copolymers and Their Carbohydrate Specific Intracellular Delivery
Authors:
Pati, Debasis; Das, Soumen; Patil, Naganath G; Parekh, Nimisha Anant; Anjum, Dalaver H.; Dhaware, Vinita; Ambade, Ashootosh V; Sen Gupta, Sayam
Abstract:
Nano-carriers with carbohydrates on the surface represent a very interesting class of drug delivery vehicles since carbohydrates are involved in bio-molecular recognition events in vivo. We have synthesized biocompatible miktoarm star copolymers comprising glycopolypeptide and poly(ε-caprolactone) chains, using ring opening polymerization and ‘click chemistry’. The amphiphilic copolymers were self-assembled in water into morphologies such as nanorods, polymersomes and micelles with carbohydrates displayed on the surface. We demonstrate that, the formation of nanostructure could be tuned by chain length of the blocks and was not affected by the type of sugar residue. These nanostructures were characterized in detail using a variety of techniques such as TEM, AFM, cryogenic electron microscopy, spectrally resolved fluorescence imaging and dye encapsulation techniques. We show that it is possible to sequester both hydrophobic as well as hydrophilic dyes within the nanostructures. Finally, we show that these non-cytotoxic manno-sylated rods and polymersomes were selectively and efficiently taken up by MDA-MB-231 breast cancer cells demonstrating their potential as nanocarriers for drug delivery.
KAUST Department:
Imaging and Characterization Core Lab
Citation:
Tunable Nanocarrier Morphologies from Glycopolypeptide-based Amphiphilic Biocompatible Star Copolymers and Their Carbohydrate Specific Intracellular Delivery 2015 Biomacromolecules
Publisher:
American Chemical Society (ACS)
Journal:
Biomacromolecules
Issue Date:
21-Dec-2015
DOI:
10.1021/acs.biomac.5b01354
Type:
Article
ISSN:
1525-7797; 1526-4602
Additional Links:
http://pubs.acs.org/doi/10.1021/acs.biomac.5b01354
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorPati, Debasisen
dc.contributor.authorDas, Soumenen
dc.contributor.authorPatil, Naganath Gen
dc.contributor.authorParekh, Nimisha Ananten
dc.contributor.authorAnjum, Dalaver H.en
dc.contributor.authorDhaware, Vinitaen
dc.contributor.authorAmbade, Ashootosh Ven
dc.contributor.authorSen Gupta, Sayamen
dc.date.accessioned2015-12-27T13:36:27Zen
dc.date.available2015-12-27T13:36:27Zen
dc.date.issued2015-12-21en
dc.identifier.citationTunable Nanocarrier Morphologies from Glycopolypeptide-based Amphiphilic Biocompatible Star Copolymers and Their Carbohydrate Specific Intracellular Delivery 2015 Biomacromoleculesen
dc.identifier.issn1525-7797en
dc.identifier.issn1526-4602en
dc.identifier.doi10.1021/acs.biomac.5b01354en
dc.identifier.urihttp://hdl.handle.net/10754/592611en
dc.description.abstractNano-carriers with carbohydrates on the surface represent a very interesting class of drug delivery vehicles since carbohydrates are involved in bio-molecular recognition events in vivo. We have synthesized biocompatible miktoarm star copolymers comprising glycopolypeptide and poly(ε-caprolactone) chains, using ring opening polymerization and ‘click chemistry’. The amphiphilic copolymers were self-assembled in water into morphologies such as nanorods, polymersomes and micelles with carbohydrates displayed on the surface. We demonstrate that, the formation of nanostructure could be tuned by chain length of the blocks and was not affected by the type of sugar residue. These nanostructures were characterized in detail using a variety of techniques such as TEM, AFM, cryogenic electron microscopy, spectrally resolved fluorescence imaging and dye encapsulation techniques. We show that it is possible to sequester both hydrophobic as well as hydrophilic dyes within the nanostructures. Finally, we show that these non-cytotoxic manno-sylated rods and polymersomes were selectively and efficiently taken up by MDA-MB-231 breast cancer cells demonstrating their potential as nanocarriers for drug delivery.en
dc.language.isoenen
dc.publisherAmerican Chemical Society (ACS)en
dc.relation.urlhttp://pubs.acs.org/doi/10.1021/acs.biomac.5b01354en
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/10.1021/acs.biomac.5b01354.en
dc.titleTunable Nanocarrier Morphologies from Glycopolypeptide-based Amphiphilic Biocompatible Star Copolymers and Their Carbohydrate Specific Intracellular Deliveryen
dc.typeArticleen
dc.contributor.departmentImaging and Characterization Core Laben
dc.identifier.journalBiomacromoleculesen
dc.eprint.versionPost-printen
dc.contributor.institutionCReST Chemical Engineering Divisionen
dc.contributor.institutionPolymer Science and Engineering Division, CSIR National Chemical Laboratory, Dr. Homi Bhabha Road, Pune-411008, Indiaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAnjum, Dalaver H.en
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