Two domain-disrupted hda6 alleles have opposite epigenetic effects on transgenes and some endogenous targets

Handle URI:
http://hdl.handle.net/10754/592607
Title:
Two domain-disrupted hda6 alleles have opposite epigenetic effects on transgenes and some endogenous targets
Authors:
Zhang, ShouDong; Zhan, Xiangqiang; Xu, Xiaoming; Cui, Peng ( 0000-0003-3076-0070 ) ; Zhu, Jian-Kang; Xia, Yiji; Xiong, Liming ( 0000-0001-8099-0806 )
Abstract:
HDA6 is a RPD3-like histone deacetylase. In Arabidopsis, it mediates transgene and some endogenous target transcriptional gene silencing (TGS) via histone deacetylation and DNA methylation. Here, we characterized two hda6 mutant alleles that were recovered as second-site suppressors of the DNA demethylation mutant ros1–1. Although both alleles derepressed 35S::NPTII and RD29A::LUC in the ros1–1 background, they had distinct effects on the expression of these two transgenes. In accordance to expression profiles of two transgenes, the alleles have distinct opposite methylation profiles on two reporter gene promoters. Furthermore, both alleles could interact in vitro and in vivo with the DNA methyltransferase1 with differential interactive strength and patterns. Although these alleles accumulated different levels of repressive/active histone marks, DNA methylation but not histone modifications in the two transgene promoters was found to correlate with the level of derepression of the reporter genes between the two had6 alleles. Our study reveals that mutations in different domains of HDA6 convey different epigenetic status that in turn controls the expression of the transgenes as well as some endogenous loci.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Two domain-disrupted hda6 alleles have opposite epigenetic effects on transgenes and some endogenous targets 2015, 5:17832 Scientific Reports
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
15-Dec-2015
DOI:
10.1038/srep17832
Type:
Article
ISSN:
2045-2322
Additional Links:
http://www.nature.com/articles/srep17832
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, ShouDongen
dc.contributor.authorZhan, Xiangqiangen
dc.contributor.authorXu, Xiaomingen
dc.contributor.authorCui, Pengen
dc.contributor.authorZhu, Jian-Kangen
dc.contributor.authorXia, Yijien
dc.contributor.authorXiong, Limingen
dc.date.accessioned2015-12-27T13:29:07Zen
dc.date.available2015-12-27T13:29:07Zen
dc.date.issued2015-12-15en
dc.identifier.citationTwo domain-disrupted hda6 alleles have opposite epigenetic effects on transgenes and some endogenous targets 2015, 5:17832 Scientific Reportsen
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep17832en
dc.identifier.urihttp://hdl.handle.net/10754/592607en
dc.description.abstractHDA6 is a RPD3-like histone deacetylase. In Arabidopsis, it mediates transgene and some endogenous target transcriptional gene silencing (TGS) via histone deacetylation and DNA methylation. Here, we characterized two hda6 mutant alleles that were recovered as second-site suppressors of the DNA demethylation mutant ros1–1. Although both alleles derepressed 35S::NPTII and RD29A::LUC in the ros1–1 background, they had distinct effects on the expression of these two transgenes. In accordance to expression profiles of two transgenes, the alleles have distinct opposite methylation profiles on two reporter gene promoters. Furthermore, both alleles could interact in vitro and in vivo with the DNA methyltransferase1 with differential interactive strength and patterns. Although these alleles accumulated different levels of repressive/active histone marks, DNA methylation but not histone modifications in the two transgene promoters was found to correlate with the level of derepression of the reporter genes between the two had6 alleles. Our study reveals that mutations in different domains of HDA6 convey different epigenetic status that in turn controls the expression of the transgenes as well as some endogenous loci.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/articles/srep17832en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleTwo domain-disrupted hda6 alleles have opposite epigenetic effects on transgenes and some endogenous targetsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist University, Hong Kong, Hong Kong SAR, Chinaen
dc.contributor.institutionShanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, Chinaen
dc.contributor.institutionCollege of life sciences, Nanjing Agricultural University, Nanjing, Chinaen
dc.contributor.institutionHorticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorZhang, ShouDongen
kaust.authorCui, Pengen
kaust.authorXiong, Limingen
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