A RALDH-like enzyme involved in Fusarium verticillioides development

Handle URI:
http://hdl.handle.net/10754/584052
Title:
A RALDH-like enzyme involved in Fusarium verticillioides development
Authors:
Díaz-Sánchez, Violeta; Carmen Limón, M.; Schaub, Patrick; Al-Babili, Salim ( 0000-0003-4823-2882 ) ; Avalos, Javier ( 0000-0001-5484-2893 )
Abstract:
Retinaldehyde dehydrogenases (RALDHs) convert retinal to retinoic acid, an important chordate morphogen. Retinal also occurs in some fungi, such as Fusarium and Ustilago spp., evidenced by the presence of rhodopsins and β–carotene cleaving, retinal-forming dioxygenases. Based on the assumption that retinoic acid may also be formed in fungi, we searched the Fusarium protein databases for RALDHs homologs, focusing on Fusarium verticillioides. Using crude lysates of Escherichia coli cells expressing the corresponding cDNAs, we checked the capability of best matches to convert retinal into retinoic acid in vitro. Thereby, we identified an aldehyde dehydrogenase, termed CarY, as a retinoic acid-forming enzyme, an activity that was also exerted by purified CarY. Targeted mutation of the carY gene in F. verticillioides resulted in alterations of mycelia development and conidia morphology in agar cultures, and reduced capacity to produce perithecia as a female in sexual crosses. Complementation of the mutant with a wild-type carY allele demonstrated that these alterations are caused by the lack of CarY. However, retinoic acid could not be detected by LC-MS analysis either in the wild type or the complemented carY strain in vivo, making elusive the connection between CarY enzymatic activity and retinoic acid formation in the fungus.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
A RALDH-like enzyme involved in Fusarium verticillioides development 2015 Fungal Genetics and Biology
Publisher:
Elsevier BV
Journal:
Fungal Genetics and Biology
Issue Date:
11-Dec-2015
DOI:
10.1016/j.fgb.2015.12.005
Type:
Article
ISSN:
10871845
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S1087184515300530
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDíaz-Sánchez, Violetaen
dc.contributor.authorCarmen Limón, M.en
dc.contributor.authorSchaub, Patricken
dc.contributor.authorAl-Babili, Salimen
dc.contributor.authorAvalos, Javieren
dc.date.accessioned2015-12-17T11:54:18Zen
dc.date.available2015-12-17T11:54:18Zen
dc.date.issued2015-12-11en
dc.identifier.citationA RALDH-like enzyme involved in Fusarium verticillioides development 2015 Fungal Genetics and Biologyen
dc.identifier.issn10871845en
dc.identifier.doi10.1016/j.fgb.2015.12.005en
dc.identifier.urihttp://hdl.handle.net/10754/584052en
dc.description.abstractRetinaldehyde dehydrogenases (RALDHs) convert retinal to retinoic acid, an important chordate morphogen. Retinal also occurs in some fungi, such as Fusarium and Ustilago spp., evidenced by the presence of rhodopsins and β–carotene cleaving, retinal-forming dioxygenases. Based on the assumption that retinoic acid may also be formed in fungi, we searched the Fusarium protein databases for RALDHs homologs, focusing on Fusarium verticillioides. Using crude lysates of Escherichia coli cells expressing the corresponding cDNAs, we checked the capability of best matches to convert retinal into retinoic acid in vitro. Thereby, we identified an aldehyde dehydrogenase, termed CarY, as a retinoic acid-forming enzyme, an activity that was also exerted by purified CarY. Targeted mutation of the carY gene in F. verticillioides resulted in alterations of mycelia development and conidia morphology in agar cultures, and reduced capacity to produce perithecia as a female in sexual crosses. Complementation of the mutant with a wild-type carY allele demonstrated that these alterations are caused by the lack of CarY. However, retinoic acid could not be detected by LC-MS analysis either in the wild type or the complemented carY strain in vivo, making elusive the connection between CarY enzymatic activity and retinoic acid formation in the fungus.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1087184515300530en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Fungal Genetics and Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Genetics and Biology, 11 December 2015. DOI: 10.1016/j.fgb.2015.12.005en
dc.subjectFusarium verticillioidesen
dc.subjectRALDH enzymeen
dc.subjectRetinoic aciden
dc.subjectApocarotenoidsen
dc.subjectConidiaen
dc.subjectHyphal developmenten
dc.subjectSexual fertilityen
dc.titleA RALDH-like enzyme involved in Fusarium verticillioides developmenten
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalFungal Genetics and Biologyen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Genetics, Faculty of Biology, University of Seville, E–41012 Seville, Spainen
dc.contributor.institutionFaculty of Biology, Albert-Ludwigs University of Freiburg, Schaenzlestr. 1, D–79104 Freiburg, Germanyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAl-Babili, Salimen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.