Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea

Handle URI:
http://hdl.handle.net/10754/584004
Title:
Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea
Authors:
Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Al-Aqeel, Sarah ( 0000-0002-4918-5849 ) ; Ryu, Tae Woo; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Seridi, Loqmane ( 0000-0001-7028-3024 ) ; Ghosheh, Yanal ( 0000-0002-3733-2098 ) ; Qian, Pei-Yuan; Ravasi, Timothy ( 0000-0002-9950-465X )
Abstract:
Background The barnacle Balanus amphitrite is widely distributed in marine shallow and tidal waters, and has significant economic and ecological importance. Nauplii, the first larval stage of most crustaceans, are extremely abundant in the marine zooplankton. However, a lack of genome information has hindered elucidation of the molecular mechanisms of development, settlement and survival strategies in extreme marine environments. We sequenced and constructed the genome dataset for nauplii to obtain comprehensive larval genetic information. We also investigated iTRAQ-based protein expression patterns to reveal the molecular basis of nauplii development, and to gain information on larval survival strategies in the Red Sea marine environment. Results A nauplii larval transcript dataset, containing 92,117 predicted open reading frames (ORFs), was constructed and used as a reference for the proteome analysis. Genes related to translation, oxidative phosphorylation and cytoskeletal development were highly abundant. We observed remarkable plasticity in the proteome of Red Sea larvae. The proteins associated with development, stress responses and osmoregulation showed the most significant differences between the two larval populations studied. The synergistic overexpression of heat shock and osmoregulatory proteins may facilitate larval survival in intertidal habitats or in extreme environments. Conclusions We presented, for the first time, comprehensive transcriptome and proteome datasets for Red Sea nauplii. The datasets provide a foundation for future investigations focused on the survival mechanisms of other crustaceans in extreme marine environments.
KAUST Department:
KAUST Environmental Epigenetic Program (KEEP); Biological and Environmental Sciences and Engineering (BESE) Division; Applied Mathematics and Computational Science Program; Biosciences Core Lab; KAUST Global Collaborative Research Program
Citation:
Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea 2015, 16 (1) BMC Genomics
Publisher:
Springer Science + Business Media
Journal:
BMC Genomics
Issue Date:
15-Dec-2015
DOI:
10.1186/s12864-015-2262-1
Type:
Article
ISSN:
1471-2164
Is Supplemented By:
Kondethimmanahalli Chandramouli, Al-Aqeel, S., Taewoo Ryu, Huoming Zhang, Loqmane Seridi, Yanal Ghosheh, … Ravasi, T. (2015). Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea. Figshare. https://doi.org/10.6084/m9.figshare.c.3629591; DOI:10.6084/m9.figshare.c.3629591; HANDLE:http://hdl.handle.net/10754/624137
Additional Links:
http://www.biomedcentral.com/1471-2164/16/1063
Appears in Collections:
Articles; Applied Mathematics and Computational Science Program; Bioscience Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorAl-Aqeel, Sarahen
dc.contributor.authorRyu, Tae Wooen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorSeridi, Loqmaneen
dc.contributor.authorGhosheh, Yanalen
dc.contributor.authorQian, Pei-Yuanen
dc.contributor.authorRavasi, Timothyen
dc.date.accessioned2015-12-16T13:04:26Zen
dc.date.available2015-12-16T13:04:26Zen
dc.date.issued2015-12-15en
dc.identifier.citationTranscriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea 2015, 16 (1) BMC Genomicsen
dc.identifier.issn1471-2164en
dc.identifier.doi10.1186/s12864-015-2262-1en
dc.identifier.urihttp://hdl.handle.net/10754/584004en
dc.description.abstractBackground The barnacle Balanus amphitrite is widely distributed in marine shallow and tidal waters, and has significant economic and ecological importance. Nauplii, the first larval stage of most crustaceans, are extremely abundant in the marine zooplankton. However, a lack of genome information has hindered elucidation of the molecular mechanisms of development, settlement and survival strategies in extreme marine environments. We sequenced and constructed the genome dataset for nauplii to obtain comprehensive larval genetic information. We also investigated iTRAQ-based protein expression patterns to reveal the molecular basis of nauplii development, and to gain information on larval survival strategies in the Red Sea marine environment. Results A nauplii larval transcript dataset, containing 92,117 predicted open reading frames (ORFs), was constructed and used as a reference for the proteome analysis. Genes related to translation, oxidative phosphorylation and cytoskeletal development were highly abundant. We observed remarkable plasticity in the proteome of Red Sea larvae. The proteins associated with development, stress responses and osmoregulation showed the most significant differences between the two larval populations studied. The synergistic overexpression of heat shock and osmoregulatory proteins may facilitate larval survival in intertidal habitats or in extreme environments. Conclusions We presented, for the first time, comprehensive transcriptome and proteome datasets for Red Sea nauplii. The datasets provide a foundation for future investigations focused on the survival mechanisms of other crustaceans in extreme marine environments.en
dc.language.isoenen
dc.publisherSpringer Science + Business Mediaen
dc.relation.urlhttp://www.biomedcentral.com/1471-2164/16/1063en
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectB. amphitriteen
dc.subjectRed seaen
dc.subjectTranscriptomicsen
dc.subjectProteomicsen
dc.subjectAdaptationen
dc.titleTranscriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Seaen
dc.typeArticleen
dc.contributor.departmentKAUST Environmental Epigenetic Program (KEEP)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.identifier.journalBMC Genomicsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Life Science, The Hong Kong University of Science and Technology, Hong Kong, Hong Kongen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorAl-Aqeel, Sarahen
kaust.authorRyu, Tae Wooen
kaust.authorZhang, Huomingen
kaust.authorSeridi, Loqmaneen
kaust.authorGhosheh, Yanalen
kaust.authorRavasi, Timothyen
dc.relation.isSupplementedByKondethimmanahalli Chandramouli, Al-Aqeel, S., Taewoo Ryu, Huoming Zhang, Loqmane Seridi, Yanal Ghosheh, … Ravasi, T. (2015). Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea. Figshare. https://doi.org/10.6084/m9.figshare.c.3629591en
dc.relation.isSupplementedByDOI:10.6084/m9.figshare.c.3629591en
dc.relation.isSupplementedByHANDLE:http://hdl.handle.net/10754/624137en
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