OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

Handle URI:
http://hdl.handle.net/10754/583374
Title:
OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice
Authors:
Kim, SoonKap; Park, Hyo-Young; Jang, Yun Hee; Lee, Keh Chien; Chung, Young Soo; Lee, Jeong Hwan; Kim, Jeong-Kook
Abstract:
OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response through the modulation of three flowering-time genes ( Ehd1, Hd3a , and RFT1 ) in rice. Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11). Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice 2015 Planta
Publisher:
Springer Science + Business Media
Journal:
Planta
Issue Date:
5-Nov-2015
DOI:
10.1007/s00425-015-2426-x
Type:
Article
ISSN:
0032-0935; 1432-2048
Additional Links:
http://link.springer.com/10.1007/s00425-015-2426-x
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKim, SoonKapen
dc.contributor.authorPark, Hyo-Youngen
dc.contributor.authorJang, Yun Heeen
dc.contributor.authorLee, Keh Chienen
dc.contributor.authorChung, Young Sooen
dc.contributor.authorLee, Jeong Hwanen
dc.contributor.authorKim, Jeong-Kooken
dc.date.accessioned2015-12-08T06:55:24Zen
dc.date.available2015-12-08T06:55:24Zen
dc.date.issued2015-11-05en
dc.identifier.citationOsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice 2015 Plantaen
dc.identifier.issn0032-0935en
dc.identifier.issn1432-2048en
dc.identifier.doi10.1007/s00425-015-2426-xen
dc.identifier.urihttp://hdl.handle.net/10754/583374en
dc.description.abstractOsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response through the modulation of three flowering-time genes ( Ehd1, Hd3a , and RFT1 ) in rice. Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11). Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.en
dc.language.isoenen
dc.publisherSpringer Science + Business Mediaen
dc.relation.urlhttp://link.springer.com/10.1007/s00425-015-2426-xen
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/s00425-015-2426-xen
dc.subjectFlowering timeen
dc.subjectNUCLEAR FACTOR Y (NF-Y)en
dc.subjectPhotoperiodic floweringen
dc.subjectRiceen
dc.subjectTranscriptionen
dc.titleOsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in riceen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPlantaen
dc.eprint.versionPost-printen
dc.contributor.institutionDivision of Life Sciences, Korea University, Anam-dong 5 ga, Seongbuk-Gu, Seoul, 136-701, Republic of Koreaen
dc.contributor.institutionDepartment of Genetic Engineering, Dong-A University, Busan, 604-714, Republic of Koreaen
dc.contributor.institutionDepartment of Bioresource Engineering and Plant Engineering Research Institute, Sejong University, 98 Gunja-dong, Gwangjin-Gu, Seoul, 143-747, Republic of Koreaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKim, SoonKapen
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