The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes

Handle URI:
http://hdl.handle.net/10754/583295
Title:
The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes
Authors:
Zhao, Huayan; Lü, Shiyou; Li, Ruixi; Chen, Tao; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Cui, Peng ( 0000-0003-3076-0070 ) ; Ding, Feng ( 0000-0001-8237-4062 ) ; Liu, Pei; Wang, Guangchao; Xia, Yiji; Running, Mark P.; Xiong, Liming ( 0000-0001-8099-0806 )
Abstract:
The circularly permuted GTPase large subunit GTPase 1 (LSG1) is involved in the maturation step of the 60S ribosome and is essential for cell viability in yeast. Here, an Arabidopsis mutant dig6 (drought inhibited growth of lateral roots) was isolated. The mutant exhibited multiple auxin-related phenotypes, which included reduced lateral root number, altered leaf veins, and shorter roots. Genetic mapping combined with next-generation DNA sequencing identified that the mutation occurred in AtLSG1-2. This gene was highly expressed in regions of auxin accumulation. Ribosome profiling revealed that a loss of function of AtLSG1-2 led to decreased levels of monosomes, further demonstrating its role in ribosome biogenesis. Quantitative proteomics showed that the expression of certain proteins involved in ribosome biogenesis was differentially regulated, indicating that ribosome biogenesis processes were impaired in the mutant. Further investigations showed that an AtLSG1-2 deficiency caused the alteration of auxin distribution, response, and transport in plants. It is concluded that AtLSG1-2 is integral to ribosome biogenesis, consequently affecting auxin homeostasis and plant development.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Biosciences Core Lab
Citation:
The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes 2015, 66 (21):6863 Journal of Experimental Botany
Publisher:
Oxford University Press (OUP)
Journal:
Journal of Experimental Botany
Issue Date:
13-Aug-2015
DOI:
10.1093/jxb/erv391
Type:
Article
ISSN:
0022-0957; 1460-2431
Additional Links:
http://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/erv391
Appears in Collections:
Articles; Bioscience Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhao, Huayanen
dc.contributor.authorLü, Shiyouen
dc.contributor.authorLi, Ruixien
dc.contributor.authorChen, Taoen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorCui, Pengen
dc.contributor.authorDing, Fengen
dc.contributor.authorLiu, Peien
dc.contributor.authorWang, Guangchaoen
dc.contributor.authorXia, Yijien
dc.contributor.authorRunning, Mark P.en
dc.contributor.authorXiong, Limingen
dc.date.accessioned2015-12-06T12:50:35Zen
dc.date.available2015-12-06T12:50:35Zen
dc.date.issued2015-08-13en
dc.identifier.citationThe Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes 2015, 66 (21):6863 Journal of Experimental Botanyen
dc.identifier.issn0022-0957en
dc.identifier.issn1460-2431en
dc.identifier.doi10.1093/jxb/erv391en
dc.identifier.urihttp://hdl.handle.net/10754/583295en
dc.description.abstractThe circularly permuted GTPase large subunit GTPase 1 (LSG1) is involved in the maturation step of the 60S ribosome and is essential for cell viability in yeast. Here, an Arabidopsis mutant dig6 (drought inhibited growth of lateral roots) was isolated. The mutant exhibited multiple auxin-related phenotypes, which included reduced lateral root number, altered leaf veins, and shorter roots. Genetic mapping combined with next-generation DNA sequencing identified that the mutation occurred in AtLSG1-2. This gene was highly expressed in regions of auxin accumulation. Ribosome profiling revealed that a loss of function of AtLSG1-2 led to decreased levels of monosomes, further demonstrating its role in ribosome biogenesis. Quantitative proteomics showed that the expression of certain proteins involved in ribosome biogenesis was differentially regulated, indicating that ribosome biogenesis processes were impaired in the mutant. Further investigations showed that an AtLSG1-2 deficiency caused the alteration of auxin distribution, response, and transport in plants. It is concluded that AtLSG1-2 is integral to ribosome biogenesis, consequently affecting auxin homeostasis and plant development.en
dc.language.isoenen
dc.publisherOxford University Press (OUP)en
dc.relation.urlhttp://jxb.oxfordjournals.org/lookup/doi/10.1093/jxb/erv391en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectAtLSG1en
dc.subjectArabidopsisen
dc.subjectauxin homeostasisen
dc.subjectexpression patternen
dc.subjectmap-based cloningen
dc.subjectproteomicsen
dc.subjectribosome biogenesisen
dc.titleThe Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalJournal of Experimental Botanyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Chinaen
dc.contributor.institutionInstitute for Integrative Genome Biology, Center for Plant Cell Biology & Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USAen
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist University, Hong Kong, P. R. Chinaen
dc.contributor.institutionDepartment of Biology, University of Louisville, Louisville, KY 40292, USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorZhao, Huayanen
kaust.authorChen, Taoen
kaust.authorZhang, Huomingen
kaust.authorCui, Pengen
kaust.authorDing, Fengen
kaust.authorWang, Guangchaoen
kaust.authorXiong, Limingen
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