Plant-mediated interspecific horizontal transmission of an intracellular symbiont in insects

Handle URI:
http://hdl.handle.net/10754/582865
Title:
Plant-mediated interspecific horizontal transmission of an intracellular symbiont in insects
Authors:
Gonella, Elena; Pajoro, Massimo; Marzorati, Massimo; Crotti, Elena; Mandrioli, Mauro; Pontini, Marianna; Bulgari, Daniela; Negri, Ilaria; Sacchi, Luciano; Chouaia, Bessem; Daffonchio, Daniele ( 0000-0003-0947-925X ) ; Alma, Alberto
Abstract:
Intracellular reproductive manipulators, such as Candidatus Cardinium and Wolbachia are vertically transmitted to progeny but rarely show co-speciation with the host. In sap-feeding insects, plant tissues have been proposed as alternative horizontal routes of interspecific transmission, but experimental evidence is limited. Here we report results from experiments that show that Cardinium is horizontally transmitted between different phloem sap-feeding insect species through plants. Quantitative PCR and in situ hybridization experiments indicated that the leafhopper Scaphoideus titanus releases Cardinium from its salivary glands during feeding on both artificial media and grapevine leaves. Successional time-course feeding experiments with S. titanus initially fed sugar solutions or small areas of grapevine leaves followed by feeding by the phytoplasma vector Macrosteles quadripunctulatus or the grapevine feeder Empoasca vitis revealed that the symbionts were transmitted to both species. Explaining interspecific horizontal transmission through plants improves our understanding of how symbionts spread, their lifestyle and the symbiont-host intermixed evolutionary pattern.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Plant-mediated interspecific horizontal transmission of an intracellular symbiont in insects 2015, 5:15811 Scientific Reports
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
13-Nov-2015
DOI:
10.1038/srep15811
Type:
Article
ISSN:
2045-2322
Additional Links:
http://www.nature.com/articles/srep15811
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorGonella, Elenaen
dc.contributor.authorPajoro, Massimoen
dc.contributor.authorMarzorati, Massimoen
dc.contributor.authorCrotti, Elenaen
dc.contributor.authorMandrioli, Mauroen
dc.contributor.authorPontini, Mariannaen
dc.contributor.authorBulgari, Danielaen
dc.contributor.authorNegri, Ilariaen
dc.contributor.authorSacchi, Lucianoen
dc.contributor.authorChouaia, Bessemen
dc.contributor.authorDaffonchio, Danieleen
dc.contributor.authorAlma, Albertoen
dc.date.accessioned2015-11-30T06:36:54Zen
dc.date.available2015-11-30T06:36:54Zen
dc.date.issued2015-11-13en
dc.identifier.citationPlant-mediated interspecific horizontal transmission of an intracellular symbiont in insects 2015, 5:15811 Scientific Reportsen
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep15811en
dc.identifier.urihttp://hdl.handle.net/10754/582865en
dc.description.abstractIntracellular reproductive manipulators, such as Candidatus Cardinium and Wolbachia are vertically transmitted to progeny but rarely show co-speciation with the host. In sap-feeding insects, plant tissues have been proposed as alternative horizontal routes of interspecific transmission, but experimental evidence is limited. Here we report results from experiments that show that Cardinium is horizontally transmitted between different phloem sap-feeding insect species through plants. Quantitative PCR and in situ hybridization experiments indicated that the leafhopper Scaphoideus titanus releases Cardinium from its salivary glands during feeding on both artificial media and grapevine leaves. Successional time-course feeding experiments with S. titanus initially fed sugar solutions or small areas of grapevine leaves followed by feeding by the phytoplasma vector Macrosteles quadripunctulatus or the grapevine feeder Empoasca vitis revealed that the symbionts were transmitted to both species. Explaining interspecific horizontal transmission through plants improves our understanding of how symbionts spread, their lifestyle and the symbiont-host intermixed evolutionary pattern.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/articles/srep15811en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titlePlant-mediated interspecific horizontal transmission of an intracellular symbiont in insectsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDipartimento di Scienze Veterinarie e Sanità Pubblica (DIVET), Università degli Studi di Milano, 20133 Milan, Italy.en
dc.contributor.institutionLaboratory for Microbial Ecology and Technology (LabMET), Ghent University, B9000 Ghent, Belgium.en
dc.contributor.institutionDipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Università degli Studi di Torino, 10095 Grugliascoen
dc.contributor.institutionDipartimento di Scienze per gli Alimenti, la Nutrizione, l′Ambiente (DeFENS), Università degli Studi di Milano, 20133 Milanen
dc.contributor.institutionDipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, 41100 Modenaen
dc.contributor.institutionDipartimento di Scienze Agrarie e Ambientali—Produzione, Territorio, Agroenergia, Università degli Studi di Milano, 20133 Milanen
dc.contributor.institutionDipartimento di Biologia e Biotecnologie “L. Spallanzani”, Università degli Studi di Pavia, 27100 Paviaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDaffonchio, Danieleen
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