From nucleotides to DNA analysis by a SERS substrate of a self similar chain of silver nanospheres

Handle URI:
http://hdl.handle.net/10754/582544
Title:
From nucleotides to DNA analysis by a SERS substrate of a self similar chain of silver nanospheres
Authors:
Coluccio, M L; Gentile, F; Das, Gobind ( 0000-0003-0942-681X ) ; Perozziello, G; Malara, N.; Alrasheed, S; Candeloro, P; Di Fabrizio, Enzo ( 0000-0001-5886-4678 )
Abstract:
In this work we realized a device of silver nanostructures designed so that they have a great ability to sustain the surface-enhanced Raman scattering effect. The nanostructures were silver self-similar chains of three nanospheres, having constant ratios between their diameters and between their reciprocal distances. They were realized by electron beam lithography, to write the pattern, and by silver electroless deposition technique, to fill it with the metal. The obtained device showed the capability to increase the Raman signal coming from the gap between the two smallest nanospheres (whose size is around 10 nm) and so it allows the detection of biomolecules fallen into this hot spot. In particular, oligonucleotides with 6 DNA bases, deposited on these devices with a drop coating method, gave a Raman spectrum characterized by a clear fingerprint coming from the hot spot and, with the help of a fitting method, also oligonucleotides of 9 bases, which are less than 3 nm long, were resolved. In conclusion the silver nanolens results in a SERS device able to measure all the molecules, or part of them, held into the hot spot of the nanolenses, and thus it could be a future instrument with which to analyze DNA portions.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
From nucleotides to DNA analysis by a SERS substrate of a self similar chain of silver nanospheres 2015, 17 (11):114021 Journal of Optics
Publisher:
IOP Publishing
Journal:
Journal of Optics
Issue Date:
1-Nov-2015
DOI:
10.1088/2040-8978/17/11/114021
Type:
Article
ISSN:
2040-8978; 2040-8986
Additional Links:
http://stacks.iop.org/2040-8986/17/i=11/a=114021?key=crossref.f8d237c372111d186b4d9b8054c36c1b
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorColuccio, M Len
dc.contributor.authorGentile, Fen
dc.contributor.authorDas, Gobinden
dc.contributor.authorPerozziello, Gen
dc.contributor.authorMalara, N.en
dc.contributor.authorAlrasheed, Sen
dc.contributor.authorCandeloro, Pen
dc.contributor.authorDi Fabrizio, Enzoen
dc.date.accessioned2015-11-23T14:03:08Zen
dc.date.available2015-11-23T14:03:08Zen
dc.date.issued2015-11-01en
dc.identifier.citationFrom nucleotides to DNA analysis by a SERS substrate of a self similar chain of silver nanospheres 2015, 17 (11):114021 Journal of Opticsen
dc.identifier.issn2040-8978en
dc.identifier.issn2040-8986en
dc.identifier.doi10.1088/2040-8978/17/11/114021en
dc.identifier.urihttp://hdl.handle.net/10754/582544en
dc.description.abstractIn this work we realized a device of silver nanostructures designed so that they have a great ability to sustain the surface-enhanced Raman scattering effect. The nanostructures were silver self-similar chains of three nanospheres, having constant ratios between their diameters and between their reciprocal distances. They were realized by electron beam lithography, to write the pattern, and by silver electroless deposition technique, to fill it with the metal. The obtained device showed the capability to increase the Raman signal coming from the gap between the two smallest nanospheres (whose size is around 10 nm) and so it allows the detection of biomolecules fallen into this hot spot. In particular, oligonucleotides with 6 DNA bases, deposited on these devices with a drop coating method, gave a Raman spectrum characterized by a clear fingerprint coming from the hot spot and, with the help of a fitting method, also oligonucleotides of 9 bases, which are less than 3 nm long, were resolved. In conclusion the silver nanolens results in a SERS device able to measure all the molecules, or part of them, held into the hot spot of the nanolenses, and thus it could be a future instrument with which to analyze DNA portions.en
dc.language.isoenen
dc.publisherIOP Publishingen
dc.relation.urlhttp://stacks.iop.org/2040-8986/17/i=11/a=114021?key=crossref.f8d237c372111d186b4d9b8054c36c1ben
dc.rightsContent from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.en
dc.titleFrom nucleotides to DNA analysis by a SERS substrate of a self similar chain of silver nanospheresen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalJournal of Opticsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionBioNEM, University Magna Graecia of Catanzaro, 88100, Catanzaro, Italyen
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University of Naples, 80125, Naples, Italyen
dc.contributor.institutionDepartment of Health Science, University Magna Graecia of Catanzaro, 88100, Catanzaro, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
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